G142D Protein (ECD, His & AVI Tag), Biotinylated

参考价:¥4520
供货周期: 一周
品牌: 博尔森
规格: 20 μg
货号: BES22622RP
CAS号:
上海博尔森生物科技有限公司
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SARS-CoV-2 Spike S1+S2 (G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H, H1101D) Protein (ECD, His & AVI Tag), Biotinylated


纯度:> 85 % as determined by SDS-PAGE.


内毒素:< 1.0 EU per μg protein as determined by the LAL method.


生物活性:Testing in progress


蛋白构建:A DNA sequence encoding the SARS-CoV-2 Spike S1+S2 (YP_009724390.1, with mutations G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H, H1101D) (Met1-Pro1213) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. The mutations were identified in the SARS-CoV-2 variant (known as B.1.617) which emerged in the India.


NP No.:YP_009724390.1


表达宿主:Baculovirus-Insect Cells


种属:SARS-CoV-2


预测 N 端:Val 16


分子量:The recombinant SARS-CoV-2 Spike S1+S2 consists of 1224 amino acids and predicts a molecular mass of 136.29 kDa.


缓冲液:Lyophilized from sterile 20mM Tris, 300mM NaCl, 10% glycerol, pH 8.0.

Please contact us for any concerns or special requirements.

Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.


运输方式:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.


稳定性 & 储存条件:Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.


复溶:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

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