Abstract:

 Tune the metabolic drivers of immune cell potency, fate and fitness
    The unique metabolic properties and liabilities of tumors are leading to fresh perspectives of how to intervene in this chronic, progressive disease. Much has been learned in the last decade about metabolic reprogramming in cancer. By applying this knowledge to infiltrating immune cells we are now beginning to appreciate the metabolic drivers of immune cell fitness, specifically stable mitochondrial function that can perhaps reverse exhaustion phenotypes by enhancing survival and function in hypoxic and nutritionally depleted tumor microenvironments 
Agilent’s cell analysis portfolio uses label-free, time resolved technology to detect discrete changes in cell fate and behavior, providing a window into the critical functions driving cell signaling, proliferation, activation, toxicity and biosynthesis. Move beyond analyzing what your cells are, and reveal a clearer picture of what they do.

This talk will showcase purpose-built applications that enable real-time measures of immune cell function, fate and fitness. This tool bench provides the capability to measure, engineer and control immune cell function, thus enabling translational researchers and developers to achieve the necessary level of therapeutic potency and safety.

开发过程中各阶段的测量工具： 

– 利用活细胞成像技术进行实时细胞杀伤的动态 分析 
– 免疫细胞命运和适应性的动态分析 

– 采用流式细胞仪进行多参数免疫表型分析
– 稳定的靶标基因编辑