Prediction of cell focal adhesions using Fourier transform infrared spectroscopy
Biomaterials’ surface properties elicit diverse cellular responses in biomedical and biotechnological applications. Predicting cell behaviour on polymeric surface is an ongoing challenge due to its complexity. This work proposes a novel modelling methodology based on attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy. Spectra were collected on wetted polymeric surfaces to incorporate both surface chemistry and information on water-polymer interactions. The best model was seen to predict total area of focal adhesions (FAs) with coefficient of determination for prediction (〖R^2〗_P) of 0.94 and root mean square errors of prediction (RMSEP) of 2.57 µm when tested on an independent experimental set. This work offers new insights into our understanding of cell-biomaterial interactions. The presence of carboxyl groups in polymers promoted larger adhesion areas, yet the formation of carbonyl-to-water interaction decreased adhesion areas. Surface wettability, which was related to water-polymer interaction, was proven to highly influence cell adhesion.
487
2020-11-13
投诉
×
×