Pushing the limit of vibrational imaging resolution through temporal features
Vibrational imaging offers molecular contrast and provides unprecedented insights into life and materials science. However, the resolution has been limited to optical diffraction limit, especially with near-infrared lasers. To tackle this challenge, vibrational absorption-based imaging has been revitalized in recent years via photothermal effect. We utilize the temporal features of the transient heating/cooling cycles of photothermal lensing, and extract subtle spatial features below the diffraction limit of the probe beam, termed photothermal relaxation localization (PEARL). In contrast to existing techniques, PEARL's power dependence is highly linear, yielding higher signal levels without requiring high peak power. As demonstrated experimentally, the improvement of resolution reveals distinct locations of subcellular droplets and provide new insights into cellular liquid droplets dynamics. Collectively, super-resolution vibrational imaging promises various applications, especially for small molecule metabolites with low labeling specificity.
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2023-12-07
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