颅内测量局部脑组织氧水平PbtO2,已成为重症监护室诊断患者严重创伤和缺血损伤的实用工具。本文作者们在动物模型中的初步工作支持这样一种假设,即PbtO2的多部位深度电极记录,可能会给外科医生和重症监护提供者提供有关大脑生存能力和更好恢复能力的所需信息。本文介绍了FDA批准的、市售的、临床级深度记录电极的表面形态表征和对氧检测分析性能的电化学评估,该电极包括12个Pt记录部位。 发现记录位点的表面由光滑的铂薄膜组成,并且通过循环伏安法在酸性和中性电解质中评估的电化学行为是典型的多晶铂表面。通过电化学活性表面的测定进一步证实了铂表面的光滑度,确认粗糙度因子为0.9。最佳工作电位为?0.6 V vs.Ag/AgCl,传感器显示适合体内PbtO2测量的灵敏度和检测限值。基于报道的Pt对O2电还原反应的催化性质,本文提出这些探针可以重新用于人体大脑中PbtO2的多点监测。
From each patient one biopsy was thawed. The thawed tissue was cut into two comparable parts and the wet weight of both parts was measured. Both parts were dried overnight at 100?C, after which they were weighed again in order to measure dry weights. One part was dissolved in nitric acid and consequentially diluted, and sodium content of this solution was mea sured by way of flame spectrometry. The other part was ashed to measure nitrogen via thermal conduction (Dumatherm Nitrogen/Protein analyzer, C. Gerhardt). Nitrogen content of the ashed biopsies was measured as a parameter for protein content of the tissue. This protein content was used to correct for subcutaneous fat, since the assumption was that sodium is stored in skin instead of in fat, and protein is largely absent from fat tissue. Sodium content of the skin is expressed as mmol sodium per mg dryweight or per mg protein.从每个患者中解冻一个活检。将解冻的组织切割成两个可比较的部分,并测量两个部分的湿重。两个部分在100摄氏度干燥过夜,之后再次称重以测量干重。将一部分溶解在硝酸中并随后稀释,并且通过火焰光谱法测量该溶液的钠含量。另一部分用于通过热传导测量氮(格哈特公司 杜马森Dumatherm燃烧法全自动氮/蛋白质测定仪)。测量灰化后的活检的氮含量作为组织蛋白质含量的参数。这种蛋白质含量用于皮下脂肪,因为假设钠储存在皮肤中而不是脂肪中,并且脂肪组织中基本上不存在蛋白质。皮肤的钠含量表示为每mg干重或每mg蛋白质的mmol钠。