热滴定量热仪

Microcalorimetric instruments and methods are now essential tools for the general understanding of binding thermodynamics of biological macromolecules and specific biological systems. Provided that the enthalpy of binding is of appropriate magnitude, it is possible to determine affinities, KD, in the order of 10 nM (K=l08 M-1) for 1:1 complexes. One of the most important issues with ITC is the time needed to obtain a full binding isotherm. ITC inherently suffers from the relatively small number of binding experiments that are possible to perform per day. The focus has so far been on improving calorimetric response time of the instruments by various means, like assigning instrumental time constants and applying Tian’s equation to dynamically correct the raw calorimetric signal from a fast step-wise titration. Notably, nothing has so far been done to improve the experimental procedure. At the moment 2-3 h is needed to complete an ITC binding experiment. The number of data points that can be obtained, 20-30 points, limits the range of equilibrium constants that can be resolved from the data. For ITC this range is 1 ≤ KCM ≤ 1000, where K is the equilibrium constant and CM is the concentration of the reactant in the vessel. In this Application Note we show the possibility to shorten the time of an ITC experiment by slow continuous titration into the calorimetric vessel, cITC.

个性化的用户界面个人主界面灵活的用户权限管理方式自动滴定剂识别热插接和即插即用根据用户的需要度身定制 梅特勒托利多超越系列滴定仪，有着优良的性价比和高度自动化的特点。根据型号不同，可同时连接多个滴定管和传感器，进行自动多步滴定、返滴定等各种复杂滴定测试。选用不同电极还可进行各种类型的滴定，例如：酸碱滴定、络合滴定、非水滴定、氧化还原滴定，甚至卡尔费休水分测定、气体水分测定等。

使用热成像仪方便地对低中高压系统的热状态进行评估识别具有热异常的电气部件或连接点