活体成像分析

BTX 活体专业电极， 专为您的活体电穿孔实验精心设计。BTX为您的活体实验提供了种类繁多，不同尺寸的专业电极，为您的活体电穿孔实验提供最大便利的同时，让您获得最高的转染效率。

活体转基因--钳状电极用于体内药物/基因/蛋白质传递及基因治疗电极．活体转基因--钳状电极Tweezertrodes是一种可复用的小钳状电极，主要用于体内的药物或基因的传递。电极是一个标准的11.5厘米的小钳子，其尖部为嵌入的不锈钢圆盘状电极，两盘的间隙可调至2厘米，并有一个阳极指示器。活体转基因--钳状电极Tweezertrodes电极有两种规格，45-0118为直径：7mm的电极盘，45-0119为直径：10mm的电极盘当把感兴趣的分子注射到体内后，用电极盘夹住相关组织，然后给出电脉冲，使孔道开始形成，将分子整合到直接与电极盘接触的组织细胞内。Tweezertrodes are reusable, tweezer-style in vivo electrodes fordrug or gene delivery in animals. Tweezertrodes consist of astandard 11.5 cm tweezer that has been modified with stainlesssteel circular or disk electrodes at the tip. The gap between theelectrode disks may be adjusted from under 1 mm to over 2 cm.Tweezertrodes come in two models. Model 520 has an electrode diskdiameter of 7 mm and Model 522 has a diameter of 10 mm.Tweezertrodes may be cleaned with a mild detergent and sterilizedwith ethanol or ethylene oxide. These electrodes are connected to apulse generator with the Model 524 Connection Cable, and arecompatible with most BTX Electroporation SystemApplicationsTweezertrodes may be used for many in vivo applications, includinggene and drug delivery. Following localized or systemic injectionof the molecule of interest, the Tweezertrode electrode disks areused to grasp the tissue of interest. An electroporation pulse isthen given, initiating pore formation and incorporation of themolecule into the cells of the tissue in direct contact with theelectrode disks. Suzuki et al. used Tweezertrodes to deliver aGreen Fluorescent Protein (GFP) gene into rat liver. The authorsdemonstrated successful gene transfer and expression in situ.1,2Saito and Nakatsuji used a similar electrode to introduce plasmidDNA during ex-utero and in-utero electroporation.7 Sato et al. usedthe Tweezertrode with the T820 to introduce EGFP expressing plasmidin mouse testis.8 Tweezertrodes complement BTX Genetrode Models508, 510, Caliper Electrode Models 384 and 384L, 2-Needle ArrayModels 530 and 532 as the newest addition to our in vivo electrodeline. The Tweezertrodes can be used to reproduce applicationsrequiring the caliper electrodes, including gene therapy,transdermal drug delivery and electroporation therapy.3,4,5,6 Technical SpecificationsStandard Capabilities Voltage Range: 0V - 200V (Do not use AC current) Pulse Length Range: 1 μsec - 200 msec Physical Characteristics Model 520 Tweezertrode Length: 11.5 cm Electrode Diameter: 7 mm Electrode Material: Stainless steel Model 522 Tweezertrode Length: 11.5 cm Electrode Diameter: 10 mm Electrode Material: Stainless steel Generator Compatibility ECM T820, 630, 830, 2001 Ordering InformationSystem Model Available Configurations 520 Tweezertrode 520Tweezer Electrode with stainless steel plated 7 mm diameterelectrode disk for in vivo applications 523 Tweezertrode 523Tweezer Electrode with stainless steel plated 10mm diameterelectrode disk for in vivo applications Accessory Model Description 524 Tweezertrode Connection Cable ReferencesSuzuki et al., Direct Gene Transfer into Rat Liver Cells by in vivoElectroporation, FEBS Letters, 425: 436-440 (1998) BTX Electroporation Protocol PR0363 (1998) Zhang et al., In vivo transdermal delivery of large molecules bypressure-mediated electroincorporation and electroporation: a novelmethod for drug and gene delivery, Bioelectrochemistry andBioenergetics, 42: 283-292 (1997) Dev, Giordano and Brown, In vivo delivery of gene to rabbit carotidartery by electroporation, Third U.S. / Japan Symposium on DrugDelivery Systems (Abstr.), 38 (1995) Dev, Killing cancer cells with a combination of pulsed electricfields and chemotherapeutic agents, Cancer Watch, 3: 12-14 (1994)BTX Electroporation Therapy Protocols ECT001-ECT005 (1995) Saito and Nakatsuji, Developmental Biology, 240: 237-246 (2001)Sato et al., Molecular Reproduction and Development, 61: 49-56(2002)

超分辨成像缓冲体系试剂盒用途：本试剂盒提供超分辨荧光成像所需的全套成像缓冲体系。规格：包含可进行50次独立实验的用量，每次实验可检测10个样品。1）试剂成分和pH稳定，能保证长时间的成像效果，且对样品无损伤；2）试剂盒内所有内容物均采用灭菌处理，无污染，且不产生背景荧光；3）方便快捷，用户只需提供样品，加入本品即可进行超分辨荧光成像；4）操作简单，即开即用，无需复杂配制过程，无需专业人员即可完成。