微量热滴定量热仪

Sensys Evo微量热仪与气体吸附仪联用；Sensys Evo还可以加载Setaram独有的全对称光电天平实现TG-DSC联用，通过TG法对气体吸附进行定量

本实验将使用汇诚仪器的热重分析仪TGA-601对复合改性硼粉进行微量检测能更准确地评估样品的失重率及热稳定性。

铅离子. 本文对重金属离子进行了适当的富集, 降低其检测下限, 为采用微量热法进行重金属离子的快速检测提供了理论依据.

近年来已有越来越多利用微量热仪器研究食品相关课题发表，包含天然物的分离纯化、食品加工程序优化、食品添加剂、食品污染及毒性评估等。基于仪器的高灵敏度及能够量测活体生物生长代谢能量的优点，已有许多研究植物细胞、动物细胞或微生物（细菌、真菌、病毒等）相关文献发表在不同领域。本短文乃是基于生长代谢能量之量测，在食品加工上的应用简介。

2019年底在武汉爆发的新冠肺炎(2019 novel coronavirus disease, COVID-19)造成国内外乃至全球的大冲击，无论是医药、经济、贸易及各产业体系均受到巨大考验。藉由全球科学家们日以继夜的努力研究，发现新冠肺炎可能的致病基理；其中较为关键的是新冠病毒感染主要是藉由新冠病毒上的S蛋白(spike protein)，专一性的识别攻击人类细胞受体ACE2 (angiotensin converting enzyme 2)进而引起后续的反应。相较于同是冠状病毒的SARS，COVID-19识别人类细胞受体的专一性更强高出20倍1-3。鉴于新冠肺炎启发，本文将回顾利用等温滴定量热法(isothermal titration calorimetry, ITC)及差式扫描量热法(differential scanning calorimetry, DSC)研究病毒相关的技术文献。期望能对抗病毒药物开发、疫苗、检验试剂及基础研究等领域，提供有用的信息。

评价深水钻井液气体水合物抑制性的新方法-DSC技术，利用高压微量热仪的特点研究甲烷气体在不同液体介质中生成气体水合物的规律，建立了钻井液气体水合物抑制性实例。

电位滴定法测定二氯甲烷的微量酸值应用领域： 制药用户信息：关键词： 二氯甲烷，酸值绑定：905

电位滴定法测定二氯甲烷的微量酸值应用领域： 制药用户信息：关键词： 二氯甲烷，酸值绑定：905

本申请说明证明了毛细管池和护套用于核糖核酸酶a的热倾斜研究。关键词：圆二色性，毛细管池，微量分析，热变性，核糖核酸酶A，变性蛋白分析软件，变性温度，J-1500

使用MicroDSC7 Evo进行塑晶压卡效应研究的典型系统配置示意图，微量热仪本身采用半导体制冷，无需液氮等外部冷源，高压环境由外部的高压气体控制面板控制，通过1/16管路与样品池连通。

微量氯离子一直是众多企业产品中必测的项目之一，通过近一段时间接触的样品了解到，需检测的微量氯离子含量多在1ppm-50ppm间。本文采用氯化钠水溶液作为样品，排除样品中的其他成分对电极信号的干扰，寻找其检测限和定量限。实验结果证明，T960的检测限是2ppm，定量限是5ppm。

电位滴定法测定二氯甲烷的微量酸值应用领域： 制药用户信息：关键词： 二氯甲烷，酸值绑定：905

电位滴定法测定二氯甲烷的微量酸值应用领域： 制药用户信息：关键词： 二氯甲烷，酸值绑定：905

Interactions between water and component materials take place in all steps of dosage from manufacture. These interactions can affect the mechanical, physical and chemical properties and consequently also the behaviour of the material of interest.

含量测定：取本品约0.12g,精密称定，加冰醋酸20ml微热使溶解，放冷，加醋酐30ml,照电位滴定法用高氯酸滴定液(0.1mol/L)滴定，并将滴定的结果用空白试验校正。每1ml高氯酸滴定液(0.1mol/L)相当于16.86mg的C12H17ClN4OS.HCl.

Microcalorimetric instruments and methods are now essential tools for the general understanding of binding thermodynamics of biological macromolecules and specific biological systems. Provided that the enthalpy of binding is of appropriate magnitude, it is possible to determine affinities, KD, in the order of 10 nM (K=l08 M-1) for 1:1 complexes. One of the most important issues with ITC is the time needed to obtain a full binding isotherm. ITC inherently suffers from the relatively small number of binding experiments that are possible to perform per day. The focus has so far been on improving calorimetric response time of the instruments by various means, like assigning instrumental time constants and applying Tian’s equation to dynamically correct the raw calorimetric signal from a fast step-wise titration. Notably, nothing has so far been done to improve the experimental procedure. At the moment 2-3 h is needed to complete an ITC binding experiment. The number of data points that can be obtained, 20-30 points, limits the range of equilibrium constants that can be resolved from the data. For ITC this range is 1 ≤ KCM ≤ 1000, where K is the equilibrium constant and CM is the concentration of the reactant in the vessel. In this Application Note we show the possibility to shorten the time of an ITC experiment by slow continuous titration into the calorimetric vessel, cITC.

伊利纯牛奶和高钙奶的EDTA 标准液消耗体积平均值分别为1.616、1.450mL，RSD 分别为0.16% 和0.32%，7 次测定的回收率为98%～101.5%。与传统的消化方法及常量滴定法相比，该法具有简单、快速、节省试剂、环境污染小等优点。

A common technique for sampling organic solvents in workplace air is the use of preconcentration of vapors on adsorbents. The most widely used adsorbent is activated carbon, due to its large sampling capacity. After desorption (thermal or displacement by other solvents such as carbon disulfide), analysis is made by gas chromatography. In order to avoid breakthrough, the collected amount is usually 10% of the maximum capacity (total pore volume).

蛋白质研究：测定蛋白质变性及聚集温度，反应热，评估稳定性及相容性β -乳球蛋白在不同环境下的变性温度通过改变环境参数，评价蛋白质在不同pH、盐度及浓度条件下的变性行为。

Materials such as coals, coal products (e.g., chars, briguettes) and activated carbons are a self-heating hazard in storage and transportation. Accordingly, work was carried out in the 1970’s (1,2) largely at the Fire Research Station in the UK, which led to the formulation of a test (3,4) for predicting whether or not a particular coal or carbon material was a self-heating hazard. The test wich is very widely used up to the present time, focuses on the quantity critical ignition temperature, and the present author has shown (5) that this is not the most suitable quantity around which to build such a test. The critical ignition temperature provides an indication of self-heating hazards only in the limit where all susceptible materials have the same activation energy (usual symbol E, units J mol-1) for their reaction with atmospheric oxygen. This is very far from the truth, and consequently the tests are unreliable.

一、实验原理 天然有机物（如蛋白质和氨基酸等化合物）的总氮量通常用微量凯氏定氮法（micro-kjeldahl method）来测定。当被测的天然含氮化合物与浓硫酸共热时分解出氨，氨与硫酸反应生成硫酸铵，此过程称为消化。由于消化过程进行缓慢，实验中常添加硫酸钾和硫酸铜的混合物来促进，硫酸铜是催化剂，硫酸钾可提高消化液的沸点。氧化剂过氧化氢也能加速反应。消化完成后，在凯氏定氮仪中加入强碱碱化消化液，使碳酸铵分解出氨。用水蒸汽蒸馏法将氨蒸入无机酸溶液中，然后再用标准酸溶液进行滴定。

使用Sensys Evo进行超临界CO2研究。Sensys Evo快速微量热仪基于Setaram独有的卡尔维式三微量热原理，在高压环境中也可以保证极高的量热准确度；同时兼具温度范围宽广，升降温速度快等优点，因此可以将其视为使用微量热仪核心科技，以DSC模式工作的高效能量热仪。

The fled of biologics, i.e. proteins, particularly monoclonal antibodies (mAbs), as pharmaceutical drugs, is the fastest growing segment in the biopharmaceutical industry. Therapeutic formulations of monoclonal antibodies usually require concentrations on the order of 100 mg/mL or more, a condition that exacerbates protein denaturation and aggregation tendencies. Selecting the monoclonal antibody with the best denaturation/aggregation profile and identifying the solution conditions (formulation) that maximize the long term stability of the antibody are major goals in the development process. To accomplish these goals, researchers routinely measure different aspects of protein denaturation and aggregation using various complementary or orthogonal techniques. Some techniques measure the conformational stability of the protein (e.g., differential scanning calorimetry, differential scanning ?uorometry, isothermal chemical denaturation) and other techniques measure aggregation after lengthy sample incubation (e.g., size exclusion chromatography, light scattering). Since the expected long term stability is longer than one year, incubation times are usually reduced by accelerating denaturation/aggregation process by, for example, increasing the incubation temperature or stressing the sample in different ways. It would be highly desirable for a more efficient development of new therapeutic mAbs, to have access to a technique that provides the rate of denaturation/aggregation fast and accurately. In this technical note, we introduce the Thermal Activity Monitor (TAM) as a way to measure the rates of denaturation and aggregation of monoclonal antibodies and other proteins at constant temperature.

Biofilms are defined as “bacteria growing on a surface as single or multi-layered communities”. Over the past several years the importance of biofilms in research has been increasingly growing as scientists realized that many, if not a large majority, of microorganisms exist naturally as biofilms. Indeed, biofilms studies are common in water science (natural waters or waste waters), environmental studies (biofilm formation on rocks), material science (antifouling surfaces) and finally medical or biomedical studies (infections, implantology).The study of biofilms has evolved with the development of many staining, molecular, or microscopy techniques. However, most of these techniques are destructive and therefore only provide endpoint measurements. Consequently, the metabolism and the dynamic behavior of biofilms remains mostly inaccessible to researchers.In this context, isothermal microcalorimetry is a valuable tool enabling investigation of development and metabolic activities of growing or mature biofilms. Several techniques combined with an isothermal calorimeter can be used to study biofilms and this application note aims at summarizing these techniques and providing some tips and tricks.

本方法的目的是探索并建立一种快速高效的热裂解气质联用方法，定性和定量测定环境水体和土壤中的微塑料。单靠一种分析技术是不可能提供微塑料的全貌，热裂解气质连用法相对于其他微塑料的测定方法，具有更广泛的适用性，虽然红外光谱法可测量微塑料的颗粒数量，但是基于质量的浓度更有利于数据的分析比较，排除不同颗粒大小的影响。热裂解气质联用方法具有可准确定量分析的特点，特别是由于GCMS具有高灵敏度的能力，可对低浓度微塑料进行分析，另外此方法还可以分析微塑料中的添加和吸附的微量有机成分。

对核酸和蛋白质纯度进行 测定时，通常使用紫外可见分光光度计。由于样品体积小，所以需要使用微量样品池。本文介绍使用超微量样品池TrayCell，体积3-5uL，相比以前一般使用的25uL样品池，能够准确完成高精度定量。

本方法的目的是探索并建立一种快速高效的热裂解气质联用方法，定性和定量测定环境水体和土壤中的微塑料。单靠一种分析技术是不可能提供微塑料的全貌，热裂解气质连用法相对于其他微塑料的测定方法，具有更广泛的适用性，虽然红外光谱法可测量微塑料的颗粒数量，但是基于质量的浓度更有利于数据的分析比较，排除不同颗粒大小的影响。热裂解气质联用方法具有可准确定量分析的特点，特别是由于GCMS具有高灵敏度的能力，可对低浓度微塑料进行分析，另外此方法还可以分析微塑料中的添加和吸附的微量有机成分。

Continuous isothermal titration calorimetry (cITC) is ideal for studying rapid binding events typical of many biological recognition and binding reactions. The binding constant, stoichiometry and enthalpy of the reaction are accurately determined in significantly less time than that required using incremental isothermal titration calorimetry.

精密称取盐酸苯乙双胍样品约0.1g，置于干燥的滴定杯中，加冰醋酸20mL与醋酸酐10mL微热溶解后，放冷，照电位滴定法，用高氯酸-冰醋酸溶液滴定，记录消耗滴定剂的体积数。该方法简单易操作，测量结果准确。

The development of monoclonal antibody (Mab) based drugs hasundergone considerable growth since the release of the first Mab in the mid-eighties. Intuitive biophysical tools that examine and quantify interactions and conformational dynamics are important in demonstrating and understanding local and global conformational changes.The structure of antibodies as well as other macromolecules is related to its function and efficacy therefore control over these parameters is essential. The association of antigens to antibodies or of antibodies to delivery assemblies such as liposomes or gold nanoparticles can beaddressed by isothermal titration calorimetry (ITC) while the folding of antibodies can be assayed using differential scanning calorimetry (DSC). Both techniques offer the advantage of using native solutions and both instruments are offered by TA Instruments.