转基因标签法

仪器简介：活体转基因--钳状电极用于体内药物/基因/蛋白质传递及基因治疗电极Tweezertrodes电极是一种可复用的小钳状电极，主要用于体内的药物或基因的传递。电极是一个标准主要特点：活体转基因--钳状电极用于体内药物/基因/蛋白质传递及基因治疗电极Tweezertrodes电极是一种可复用的小钳状电极，主要用于体内的药物或基因的传递。电极是一个标准的11.5厘米的小钳子，其尖部为嵌入的不锈钢圆盘状电极，两盘的间隙可调至2厘米，并有一个阳极指示器。Tweezertrodes电极有两种规格，45-0118为直径：7mm的电极盘，45-0119为直径：10mm的电极盘.

仪器简介：活体转基因--卡钳电极384L 用于体内肌肉基因/药物传递电极.BTX Caliper 电极是一种体内可复用的卡钳状测径器电极，有多种应用，如在体内进行基因和药物的传递主要特点：活体转基因--卡钳电极384L 用于体内肌肉基因/药物传递电极.BTX Caliper 电极是一种体内可复用的卡钳状测径器电极，有多种应用，如在体内进行基因和药物的传递，也可经皮肤应用及在完整的植物上应用。该电极由一个测径器和一对末端电极组成，共有两种类型：45-0101型的末端为一对1x1cm黄铜制末端电极，适用于小动物，45-0102 型的末端为一对1.5x1.5cm或2x2cm的不锈钢制末端电极，适用于较大的区域表面，两电极间的距离可以调节。

活体转基因--钳状电极用于体内药物/基因/蛋白质传递及基因治疗电极．活体转基因--钳状电极Tweezertrodes是一种可复用的小钳状电极，主要用于体内的药物或基因的传递。电极是一个标准的11.5厘米的小钳子，其尖部为嵌入的不锈钢圆盘状电极，两盘的间隙可调至2厘米，并有一个阳极指示器。活体转基因--钳状电极Tweezertrodes电极有两种规格，45-0118为直径：7mm的电极盘，45-0119为直径：10mm的电极盘当把感兴趣的分子注射到体内后，用电极盘夹住相关组织，然后给出电脉冲，使孔道开始形成，将分子整合到直接与电极盘接触的组织细胞内。Tweezertrodes are reusable, tweezer-style in vivo electrodes fordrug or gene delivery in animals. Tweezertrodes consist of astandard 11.5 cm tweezer that has been modified with stainlesssteel circular or disk electrodes at the tip. The gap between theelectrode disks may be adjusted from under 1 mm to over 2 cm.Tweezertrodes come in two models. Model 520 has an electrode diskdiameter of 7 mm and Model 522 has a diameter of 10 mm.Tweezertrodes may be cleaned with a mild detergent and sterilizedwith ethanol or ethylene oxide. These electrodes are connected to apulse generator with the Model 524 Connection Cable, and arecompatible with most BTX Electroporation SystemApplicationsTweezertrodes may be used for many in vivo applications, includinggene and drug delivery. Following localized or systemic injectionof the molecule of interest, the Tweezertrode electrode disks areused to grasp the tissue of interest. An electroporation pulse isthen given, initiating pore formation and incorporation of themolecule into the cells of the tissue in direct contact with theelectrode disks. Suzuki et al. used Tweezertrodes to deliver aGreen Fluorescent Protein (GFP) gene into rat liver. The authorsdemonstrated successful gene transfer and expression in situ.1,2Saito and Nakatsuji used a similar electrode to introduce plasmidDNA during ex-utero and in-utero electroporation.7 Sato et al. usedthe Tweezertrode with the T820 to introduce EGFP expressing plasmidin mouse testis.8 Tweezertrodes complement BTX Genetrode Models508, 510, Caliper Electrode Models 384 and 384L, 2-Needle ArrayModels 530 and 532 as the newest addition to our in vivo electrodeline. The Tweezertrodes can be used to reproduce applicationsrequiring the caliper electrodes, including gene therapy,transdermal drug delivery and electroporation therapy.3,4,5,6Technical SpecificationsStandard CapabilitiesVoltage Range: 0V - 200V (Do not use AC current)Pulse Length Range: 1 µ sec - 200 msecPhysical CharacteristicsModel 520Tweezertrode Length: 11.5 cmElectrode Diameter: 7 mmElectrode Material: Stainless steelModel 522Tweezertrode Length: 11.5 cmElectrode Diameter: 10 mmElectrode Material: Stainless steelGenerator Compatibility ECM T820, 630, 830, 2001Ordering InformationSystem Model Available Configurations520 Tweezertrode 520Tweezer Electrode with stainless steel plated 7 mm diameterelectrode disk for in vivo applications523 Tweezertrode 523Tweezer Electrode with stainless steel plated 10mm diameterelectrode disk for in vivo applicationsAccessory Model Description524 Tweezertrode Connection CableReferencesSuzuki et al., Direct Gene Transfer into Rat Liver Cells by in vivoElectroporation, FEBS Letters, 425: 436-440 (1998)BTX Electroporation Protocol PR0363 (1998)Zhang et al., In vivo transdermal delivery of large molecules bypressure-mediated electroincorporation and electroporation: a novelmethod for drug and gene delivery, Bioelectrochemistry andBioenergetics, 42: 283-292 (1997)Dev, Giordano and Brown, In vivo delivery of gene to rabbit carotidartery by electroporation, Third U.S. / Japan Symposium on DrugDelivery Systems (Abstr.), 38 (1995)Dev, Killing cancer cells with a combination of pulsed electricfields and chemotherapeutic agents, Cancer Watch, 3: 12-14 (1994)BTX Electroporation Therapy Protocols ECT001-ECT005 (1995)Saito and Nakatsuji, Developmental Biology, 240: 237-246 (2001)Sato et al., Molecular Reproduction and Development, 61: 49-56(2002)