Dionex CarboPac PA100 糖类分析柱
Dionex CarboPac PA100 糖类分析柱用途和特点:CarboPac PA100用于从糖蛋白中切下的低聚糖、中性和有唾液酸的N-连接低聚糖;单糖连接多聚糖的异构体;线性多聚糖剖面等的分离。Dionex CarboPac PA100 糖类分析柱订货信息:分析柱CarboPac PA100 Analytical Column (2 x 250 mm)057182CarboPac PA100 Analytical Column (4 x 250 mm)043055CarboPac PA100 Column (9 x 250 mm)SP2089CarboPac PA100 Column (22 x 250 mm)SP2667保护柱CarboPac PA100 Guard Column (2 x 50 mm)057183CarboPac PA100 Guard Column (4 x 50 mm)043054Low-Picomole Oligosaccharide AnalysisOligosaccharides released from therapeutic glycoproteins can differ in size, charge, branching, and linkage. Oligosaccharides with these features can be resolved using the CarboPac? PA100. It provides the flexibility to separate oligosaccharides into broad classes depending upon the degree of sialylation, while maintaining the resolution needed for routine analysis based on linkage isomerism. Coupled with pulsed amperometric detection, the PA100 supports detection at low-picomole levels.Separation of closely related oligosaccharides, even isomers, at picomole levelsSeparation of neutral and charged oligosaccharides in the same runNo sample derivatization required for detectionThe CarboPac PA100 column is a high-resolution, strong anion-exchange column for enhanced chromatography of oligosaccharides.The Carbohydrate Membrane Desalter can be used on-line prior to fraction collection. CarboPac PA100 Column for Oligosaccharide Analysis SpecificationsDimensionsCarboPac PA100 Analytical Column:4 × 250 mmCarboPac PA100 Guard Column:4 × 50 mmCarboPac PA100 Microbore Column:2 × 250 mmCarboPac PA100 Microbore Guard Column:2 × 50 mmCarboPac PA100 Semipreparative Column:9 × 250 mmCarboPac PA100 Semipreparative Column:22 × 250 mmMaximum operating pressure4000 psi (27.9 MPa)Temperature range4–60 °CRecommended operating temperature: ambientTypical operating conditions2400 psi at 1.0 mL/min (analytical and guard columns)Recommended flow rate: 1.0 mL/minMobile phase compatibilitypH 0–14 100% compatible with common organic solvents.Ionic form eluents: sodium acetate and sodium hydroxide only.Resin composition8.5-μm-diameter ethylvinylbenzene/divinylbenzene substrate (55% cross-linking) agglomerated with 275-nm MicroBead? quaternary amine functionalized latex (6% cross-linked).Anion-exchange capacityApproximately 90 μeq/column (4 × 250 mm analytical column)Column constructionPEEK with 10-32 threaded ferrule-style end fittings.All components are nonmetallic.Separation of neutral oligosaccharide standards. Twelve commonly occurring N-linked neutral oligosaccharides are easily resolved within 20 minutes. Separation of neutral and sialylated oligosaccharide standards. Separation of trisialylated oligosaccharides. Elution of nmol Fetuin N-Linked Alditols on the CarboPac? PA100 Column. Comparison of the CarboPac? PA1 to PA100 Optimized Conditions. HPAE-PAD Analysis of Red Hook Amber Beer.ManualsDionex CarboPac PA100 Column Product ManualProduct Data SheetsCarboPac PA100 Column for Oligosaccharide Analysis Data SheetApplication NotesAN 1070: Determination of Inositol Phosphates in Dried Distillers Grains with SolublesAN 105: Glycosylation Analysis of Human Serum Transferrin Glycoforms Using Pellicular Anion-Exchange ChromatographyAN 82: Analysis of Fruit Juice Adulterated with Medium Invert Sugar from BeetsAN 67: Determination of Plant-Derived Neutral Oligo- and PolysaccharidesAN 46: Ion Chromatography: A Versatile Technique for the Analysis of BeerTechnical NotesTN 42: Glycoprotein Oligosaccharide Analysis Using High-Performance Anion-Exchange ChromatographyTN 36: Analysis of Exoglycosidase Digestions of N-Linked Oligosaccharides Using HPAE-PADTN 30: Monosaccharide and Oligosaccharide Analysis of Glycoproteins Electrotransferred onto Polyvinylidene Fluoride (PVDF) MembranesTN 20: Analysis of Carbohydrates by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD)