Alltima™ HP 色谱柱(稳定、耐用、键合相流失极低)
格雷斯/奥泰(Grace/Alltech):Alltima? HP:极其稳定耐用的高性能色谱柱, 键和相流失极低,尤其适合LCMS 使用1:更好的峰形-高纯硅胶基质消除峰拖尾问题2:更长的柱寿命-极强的耐用性,减少停机时间和使用成本3:更小的键和相流失-极低或测不到键和相流失,特别适用于LCMS 或ELSD 检测4:多种键和相和柱规格-为您优化保留、分离和分析时间提供各种选择Alltima? HP系列是我们在高纯硅胶上使用了最好的键和相技术得到的产品。这个系列有各种不同的键和相供选择,稳定耐用,性能卓越,特别适用于高难样品的分析。当您使用的是较容易流失的键和相类型如C18 Amide(C18 酰胺基)或Cyano(氰基)时,或者使用的冲洗条件较为苛刻(酸碱性较强,或柱温较高),或者配用的检测器对于键和相流失较为敏感如MS 或ELSD 时,也应该优先选择Alltima? HP以获得更好更稳定的使用效果。Alltima? HP键和相规格键和相填料基质填料形状填料粒径孔径表面积碳载量键和类型封尾?USP代码C18硅胶球形3, 5μm190A200m2/g12%单点型是L1C18 EPS硅胶球形3, 5μm190A200m2/g4%单点型是L1C18 HiLoad硅胶球形3, 5μm100A450m2/g24%单点型是L1C18 AQ硅胶球形3, 5μm100A450m2/g20%单点型是L1C18 Amide硅胶球形3, 5μm190A200m2/g12%单点型是L1C8硅胶球形3, 5μm190A200m2/g8%单点型是L7Cyano (CN)硅胶球形3, 5μm190A200m2/g4%单点型是L10Silica硅胶球形3, 5μm100A450m2/g――――无L3HILIC硅胶球形1.5, 3, 5μm120A230m2/g――――――L3在中性pH7 的条件下检测碱性很强的药物,依然能得到对称性很好的峰形。Alltima? HP C18 柱显示了pH1 和pH10 条件下极强的稳定性。pH1 酸性柱寿命测试条件:以乙腈:稀硫酸pH 1.0 (50:50)的混合溶液冲洗柱24,000 倍体积,柱温保持在60 摄氏度。最上方蓝色线条谱图为经受酸性条件冲洗后的测试谱图。pH10 碱性柱寿命测试条件:以乙腈:氨水 pH10 (50:50)的混合溶液冲洗柱24,000 倍体积,柱温保持在20 摄氏度。次上方红色线条谱图为经受碱性条件冲洗后的测试谱图。两谱图与测试进行前的初始谱图(最下方的黑色线条谱图)几乎相当。测试Alltima? HP柱和其他柱在MS 检测器上显示的键和相流失情况。最左边是未接入柱时的空白值0.82,Alltima? HP Silica,C18 和C18 Hiload 柱得到的背景噪音与空白值相当,表明没有可见的键和相流失。市场上其他产品所谓MS 专用柱如“C18 MS 1”和“C18 MS 2”都显示出不同程度的键和相流失(背景噪音高于空白值)。C18 Amide 是 “内嵌”了极性官能团的C18 键和相,容易发生水解,比常规C18 键和相的流失率高,而测试结果表明Alltima HP C18 Amide 的流失也只相当于“C18 MS 2”,远远低于市场上其他C18 Amide 柱的键和相流失程度。 氰基柱是众所周知的易流失、寿命低的色谱柱。柱寿命测试表明Alltima HP 氰基柱比市场上其他各品牌的氰基柱的耐用性好得多。分别以酸性条件和碱性条件考验柱,比较测试前后对样品的保留值变化(保留值降低的程度表示键和相流失的程度)。pH1.0 耐酸稳定性测试条件:用硫酸pH1.0:乙腈 50:50 以1mL/min 持续冲洗柱三天。pH7.5 耐碱稳定性测试条件:用20mM K2HPO4 pH7.5:乙腈 50:50 以1mL/min 持续冲洗柱三天。Alltima?HP 氰基柱的保留值降低仅为2%左右,而其它各品牌氰基柱的保留值降低幅度高达5-30%。Alltima? HP方法开发套装:每套含三根不同键和相类型但尺寸相同的色谱柱,请按您的液相系统配置、被测样品类型及复杂程度选择合适的套装。按套购买价格更为优惠。套装名称柱规格(i.d. x L)柱键和相(及各柱相应货号)货号Kit 1 – C18 mix, 3μmExpedite MS Kit 12.1 x 20mmC18, C18 Hi-Load, C18 Amide (87674, 87692, 87728)87854Analytical Kit 14.6 x 150mmC18, C18 Hi-Load, C18 Amide (87668, 87686, 87722)87850Rocket? Kit 17 x 53mmC18, C18 Hi-Load, C18 Amide (87672, 87690, 87726)87852Kit 2 – Alternate mix, 3μmExpedite? MS Kit 22.1 x 20mmC18 Hi-Load, C8, CN (87692, 87746, 87764)87864Analytical Kit 24.6 x 150mmC18 Hi-Load, C8, CN (87686, 87740, 87760)87860Rocket? Kit 27 x 53mmC18 Hi-Load, C8, CN (87690, 87744, 87762)87862Kit 3 – Polar mix, 3μmExpedite? MS Kit 32.1 x 20mmC18 EPS, C18 Amide, CN (87710, 87728, 87764)87874Analytical Kit 34.6 x 150mmC18 EPS, C18 Amide, CN (87704, 87722, 87758)87870Rocket? Kit 37 x 53mmC18 EPS, C18 Amide, CN (87708, 87726, 87762)87872常用Alltima HP 色谱柱和保护柱货号快速获取表:【有更多规格未列出。如需不同粒径、柱内径或柱长度,请垂询上海宸乔生物科技有限公司】填料柱硬件规格货号C18, 3μmCapillary: 0.150 x 150mm22155Capillary: 0.300 x 150mm22158Expedite? MS: 2.1 x 10mm87673Expedite? MS: 2.1 x 20mm87674Microbore: 2.1x 50mm87504Microbore: 2.1 x 150mm87670Analytical: 4.6 x 50mm87826Analytical: 4.6 x 150mm87668Rocket?: 7 x 33mm87671Rocket?: 7 x 53mm87672C18, 5μmMicrobore: 2.1 x 150mm87681Microbore: 2.1 x 250mm87682Analytical: 4.6 x 150mm87679Analytical: 4.6 x 250mm87680C18 AQ, 3μmCapillary: 0.150 x 150mm22562Capillary: 0.300 x 150mm22565Expedite? MS: 2.1 x 10mm87813Expedite? MS: 2.1 x 20mm87814Microbore: 2.1 x 150mm87810Analytical: 4.6 x 50mm87832Analytical: 4.6 x 150mm87808Rocket?: 7 x 33mm87811Rocket?: 7 x 53mm87812C18 AQ, 5μmMicrobore: 2.1 x 150mm87821Microbore: 2.1 x 250mm87822Analytical: 4.6 x 150mm87819Analytical: 4.6 x 250mm87820C18 EPS, 3μmExpedite? MS: 2.1 x 10mm 87709Expedite? MS: 2.1 x 20mm87710Microbore: 2.1x 50mm87508Microbore: 2.1 x 150mm87706Analytical: 4.6 x 50mm87833Analytical: 4.6 x 150mm87704Rocket?: 7 x 33mm87707Rocket?: 7 x 53mm87708C18 EPS, 5μmMicrobore: 2.1 x 150mm87717Microbore: 2.1 x 250mm87718Analytical: 4.6 x 150mm87715Analytical: 4.6 x 250mm87716C18 Hi-Load, 3μmCapillary: 0.150 x 50mm22190Capillary: 0.300 x 50mm22193Expedite? MS: 2.1 x 10mm87691Expedite? MS: 2.1 x 20mm87692Microbore: 2.1x 50mm 87506Microbore: 2.1 x 150mm87688Analytical: 4.6 x 50mm87827Analytical: 4.6 x 150mm87686Rocket?: 7 x 33mm87689Rocket?: 7 x 53mm87690C18 Hi-Load, 5μmMicrobore: 2.1 x 150mm87699Microbore: 2.1 x 250mm87700Analytical: 4.6 x 150mm87697Analytical: 4.6 x 250mm87698C18 Amide, 3μmCapillary: 0.150 x 50mm22255Capillary: 0.300 x 50mm22258Expedite? MS: 2.1 x 10mm87727Expedite? MS: 2.1 x 20mm87728Microbore: 2.1x 50mm87510Microbore: 2.1 x 150mm87724Analytical: 4.6 x 50mm 87829Analytical: 4.6 x 150mm87722Rocket?: 7 x 33mm87725Rocket?: 7 x 53mm87726C18 Amide, 5μmMicrobore: 2.1 x 150mm87735Microbore: 2.1 x 250mm87736 Analytical: 4.6 x 150mm87733Analytical: 4.6 x 250mm87734C8, 3μmCapillary: 0.150 x 150mm22481Capillary: 0.300 x 150mm22484Expedite? MS: 2.1 x 10mm87745Expedite? MS: 2.1 x 20mm87746Microbore: 2.1x 50mm87512Microbore: 2.1 x 150mm87742Analytical: 4.6 x 50mm87830Analytical: 4.6 x 150mm87740Rocket?: 7 x 33mm87743Rocket?: 7 x 53mm87744C8, 5μmMicrobore: 2.1 x 150mm87753Microbore: 2.1 x 250mm87754Analytical: 4.6 x 150mm87751Analytical: 4.6 x 250mm87752HILIC 1.5μmExpedite? MS: 2.1 x 20mm86471Rocket?: 7 x 33mm86467HILIC 3μmCapillary: 0.150 x 150mm22517Capillary: 0.300 x 150mm22520Expedite? MS: 2.1 x 10mm86473Expedite? MS: 2.1 x 20mm86475Microbore: 2.1 x 50mm86461Microbore: 2.1 x 150mm86463Analytical: 4.6 x 50mm86460Analytical: 4.6 x 150mm86462Rocket?: 7 x 33mm 86469Rocket?: 7 x 53mm86470HILIC 5μmMicrobore: 2.1 x 150mm86465Analytical: 4.6 x 150mm86464Analytical: 4.6 x 250mm86466Cyano 3μmExpedite? MS: 2.1 x 10mm87763Expedite? MS: 2.1 x 20mm87764Microbore: 2.1 x 50mm87514Microbore: 2.1 x 150mm87760Analytical: 4.6 x 50mm87825Analytical: 4.6 x 150mm87758Rocket?: 7 x 33mm87761Rocket?: 7 x 53mm87762Cyano 5μmMicrobore: 2.1 x 150mm87781Microbore: 2.1 x 250mm87782Analytical: 4.6 x 150mm87769Analytical: 4.6 x 250mm87780Silica 3μmExpedite? MS: 2.1 x 10mm87791Expedite? MS: 2.1 x 20mm87792Microbore: 2.1 x 50mm87516Microbore: 2.1 x 150mm87788Analytical: 4.6 x 50mm87831Analytical: 4.6 x 150mm87786Rocket?: 7 x 33mm87789Rocket?: 7 x 53mm87790Silica 5μmMicrobore: 2.1 x 150mm87799Microbore: 2.1 x 250mm87802Analytical: 4.6 x 150mm87797Analytical: 4.6 x 250mm87798用于保护毛细管柱的卡套与柱芯:0.100mmID 毛细管柱保护卡套,1 套GR-3710E0.300mmID 毛细管柱保护卡套,1 套GR-3710AC18 毛细管保护柱芯 3μm0.150 x 10mm225780.300 x 10mm22579C18 AQ 毛细管保护柱芯 3μm0.150 x 10mm226380.300 x 10mm22639C18 Hi-Load 毛细管保护柱芯 3μm0.150 x 10mm225880.300 x 10mm22589C18 Amide 毛细管保护柱芯 3μm0.150 x 10mm225980.300 x 10mm22599C8 毛细管保护柱芯 3μm0.150 x 10mm226480.300 x 10mm22649HILIC 毛细管保护柱芯 3μm0.150 x 10mm226580.300 x 10mm22659用于保护分析柱的卡套与柱芯:All-Guard保护柱卡套(含柱间连接件)1 套80101C18 保护柱芯 5μm4.6 x 7.5mm, 3/pk876842.1 x 7.5mm, 3/pk87683C18 AQ 保护柱芯 5μm4.6 x 7.5mm, 3/pk878242.1 x 7.5mm, 3/pk87823C18 EPS 保护柱芯 5μm4.6 x 7.5mm, 3/pk877202.1 x 7.5mm, 3/pk87719C18 Hi-Load 保护柱芯 5μm4.6 x 7.5mm, 3/pk877022.1 x 7.5mm, 3/pk87701C18 Amide 保护柱芯 5um4.6 x 7.5mm, 3/pk877382.1 x 7.5mm, 3/pk87737C8 保护柱芯5μm 4.6 x 7.5mm, 3/pk877562.1 x 7.5mm, 3/pk87755HILIC 保护柱芯5μm4.6 x 7.5mm, 3/pk864802.1 x 7.5mm, 3/pk86479Cyano 保护柱芯 5μm4.6 x 7.5mm, 3/pk877842.1 x 7.5mm, 3/pk87783Silica 保护柱芯 5μm4.6 x 7.5mm, 3/pk878042.1 x 7.5mm, 3/pk87803