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高效液相色谱法检测食品中四种尼泊金酯类防腐剂摘要建立了一种用高效液相色谱法快速检测食品中四种尼泊金酯类防腐剂的分析方法。提取液经Agilent Eclipse XDB-C18液相色谱柱(250x 4.6mm,5 um)分离,流动相为甲醇-0.02mol/L乙酸铵(45:55)进行梯度洗脱,柱温箱温度为30℃,采用可变波长紫外检测器检测,检测波长254nm。四种尼泊金酯类防腐剂在(0.5~200)ug/mL范围内线性关系良好,相关系数均大于0.995,检出限为(0.032~0.046) mg/L(S/N=3),加标回收率为(99.8~105.2)% ,RSD小于5%。该方法操作简便、快捷、灵敏、准确,适合食品中尼泊金酯类防腐剂的定量测定。关键词高效液相色谱法;食品;尼泊金酯类;防腐剂High Performance Liquid Chromatography Detection of Four Kinds of Food Parabens PreservativesAbstract: Food for the rapid detection of a high-performance liquid chromatography analysis of the four kinds of parabens preservatives. Extract Agilent Eclipse XDB-C18 HPLC Columns (250x 4.6mm, 5 um) column, the mobile phase gradient elution of methanol-0.02mol / L ammonium acetate (45:55), a variable wavelength UV detector detected, the detection wavelength of 254nm. Four kinds of parabens preservatives (0.5 to 200) ug / mL linearity in the range of a good relationship, the correlation coefficient greater than 0.995, the detection limit (0.032 to 0.046) mg / L (S / N = 3) recoveries (99.8 ~ 105.2)%, RSD less than 5%. The method is simple, fast, sensitive, accurate and suitable for leather products, parabens preservatives quantitative determination.Key words:high performance liquid chromatography;food; Parabens; preservatives1 前言尼泊金酯也称对羟基苯甲酸酯,是国际上公认的广谱、高效、低毒类防腐剂。在食品、化妆品、纺织品与皮革和医药等领域中广泛使用。尼泊金酯具有酚羟基结构,抗细菌性能比苯甲酸、山梨酸都强
请教各位高手,偶做的BOD稀释水配好放置一两天后,溶解氧就下降到5左右。想知道稀释水中的四种盐溶液的作用:磷酸盐缓冲溶液、硫酸镁溶液、氯化钙溶液、氯化铁溶液[em09512]
761做菊酯类农药标样是在丙酮溶液中,可以直接用正己烷稀释吗?还是要先吹干?