The Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector sets new benchmarks in efficient and flexible fraction collection. The fraction bed can be equipped individually with six containers holding fraction tubes in eight different sizes. Three drawers provide easy access to collected fractions even during a run. Peak- and time-based fraction modes have been extended to eight combined modes, enabling the collection of volume or time slices, and including two recovery collection modes. Trigger combinations of ultraviolet (UV) with mass-selective detection (MSD) signals now include an AND/OR logic that gives extra flexibility for challenging purifications. This Technical Overview provides a deeper insight and typical application examples of the fraction modes, including collection results.
The Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector was developed and optimized to enable efficient fraction collection with extended capacity and flexibility while providing the highest recovery and purity of the collected fractions. This Technical Overview demonstrates the performance of the 1290 Infinity II Preparative Open-Bed Fraction Collector when peak-based fraction collection is used. Using only the ultraviolet (UV) detection signal or a combination of UV and mass-selective detection (MSD) signals as a fraction trigger, purity and recovery of collected fractions are determined. Furthermore, the peak-based, collecting time slices fraction mode is discussed from a quantitative point of view.