长时间高分辨类器官光片显微镜——长时间、高通量、活细胞光片成像系统瑞士Viventis公司推出的长时间高分辨类器官光片显微镜LS系列,是一款全新的光片成像平台,主要用于活性的光敏感样品(如卵子、胚胎、类器官等)的低光毒性、高分辨率的长期成像。 长时间高分辨类器官光片显微镜是近些年发展起来的一种特殊的成像技术,它的照明光是一张与成像面平行的薄薄的光片,只有焦平面的样品被照亮,而光片上下的样品不受影响。该成像系统在细胞与组织层面的实时成像对于深入理解生物学行为至关重要。尤其适合对直径达300 μm的光敏样品(如卵母细胞,胚胎和类器官)进行长期实时高时空分辨率和低光毒性的观察与成像。★ 双侧照明光片显微镜双侧照明均可以通过软件进项控制,仅需要点击鼠标就可以控制光束的平移和旋转。光片厚度仅为1.5-6μm,且厚度可调、位置可自动校准,以适应更多的样本尺寸。配合高NA物镜,可以实现更好的穿深,更少的伪影。另外,系统配置可见激发激光器,通过检测物镜用户可对自定义样品中感兴趣的区域进行快速定位成像操作。★ 高通量,多样品同时成像Viventis长时间高分辨类器官光片显微镜可以快速对多个样品进行同时成像而无需更换样品,支持绝大多数胚胎样品并可并排摆放,方便添加培养基、加药等操作。长工作距离(样品槽尺寸50mm),同时系统可记录多个位点并连续采集。对于细胞球、类器官等本身较易漂浮的样本,Viventis也提供了较好的解决方案,采用了人工基底膜/水凝胶嵌入式等方案,实现上述样本的稳定成像。 肿瘤显微组织(成纤维细胞肿瘤细胞共培养) ★ 软件界面简洁、易于上手Viventis系统对于光片成像初学者来说操作简单,多种模式一键切换,软件界面简洁,可以帮助您快速开启光片成像之旅,打开lightsheet大门,助力科研之路。 测试数据肠道类器官发育 肿瘤显微组织(成纤维细胞肿瘤细胞共培养) 斑马鱼发育成像 发表文章2023&bull Harasimov et al., Actin-driven chromosome clustering facilitates fast and complete chromosome capture in mammalian oocytesNature Cell Biology&bull Olivetta et al., The nuclear to cytoplasmic ratio drives cellularization in the close animal relative Sphaeroforma arcticabioRvix2022&bull Ozelci et al., Deconstructing body axis morphogenesis in zebrafish embryos using robot-assisted tissue micromanipulation.Nature Communications&bull Ishihara et al., Topological morphogenesis of neuroepithelial organoids.Nature Physics&bull de Medeiros et al., Multiscale light-sheet organoid imaging frameworkNature Communication&bull Naganathan et al., Left-right symmetry of zebrafish embryos requires somite surface tension.Nature&bull So et al., Mechanism of spindle pole organization and instability in human oocytes.Science&bull Knoblochova et al., CHK1-CDC25A-CDK1 regulate cell cycle progression in early mouse embryos to protect genome integrity.bioRvix&bull Pelzer et al., Ectopic activation of the polar body extrusion pathway triggers cell fragmentation in preimplantation embryos.bioRvix2021&bull Yang et al., Cell fate coordinates mechano-osmotic forces in intestinal crypt formation.Nature cell Biology&bull He et al., Lineage recording in human cerebral organoidsNature Methods&bull Mailand et al.,Tissue Engineering with Mechanically Induced Solid-Fluid Transitions.Ad. Materials&bull Blengini et al., Aurora kinase A is essential for meiosis in mouse oocytes.Plos Genetics&bull Rohde et al., Cell-autonomous generation of the wave pattern within the vertebrate segmentation clockbioRvix2020&bull Rossi et al., Embryonic organoids recapitulate early heart organogenesis.Cell Stem Cell2019&bull Serra et al., Self-organization and symmetry breaking in intestinal organoids development.Nature&bull Dumortier et al., Fracking and Ostwald ripening position the lumen of the mouse blastocyst.Science&bull Welling et al., Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins.Elife&bull Arribat et al., Mitochondria in Embryogenesis: An Organellogenesis Perspective.Frontiers in Cell and Developmental Biology用户单位国内用户 典型国外用户
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