采用相干反斯托克斯拉满散射和其他非线性光学机制进行光学成像
In this work multimodal operation of commercially available CARSmicrospectrometer (EKSPLA CARSCOPE) equipped with a picoseconddual wavelength laser source (EKSPLA PT259) was demonstrated forthe imaging of various biological objects using coherent anti‑ StokesRaman scattering (CARS), two photon excited fluorescence (TPEF)and second harmonic generation (SHG) contrast mechanisms.Obtained CARS‑ images of yeast cell and flower pollen clearlydemonstrate label‑ free and 3D imaging capabilities of CARS technique.Multi‑ contrast images of green algae and plants containing starchdemonstrate a variety of research facilities, where the method can beapplied.