Agilent Intuvo 9000 中采用内置保留间隙柱所带来的优势远远超过在气相色谱系统中使用小内径色谱柱时所面临的潜在挑战,这些挑战还可以通过选择正确的芯片式流路和方法参数轻松克服。使用内径大于 0.2 mm 的色谱柱时,分析物谱带展宽不构成问题。如果需要使用内径小于0.2 mm 的色谱柱,可最大程度减小柱外谱带展宽的影响。与芯片式保护柱相比,使用跳线芯片产生的柱外体积更小,因此分析物谱带展宽更少。如果方法需要使用恒温柱温箱条件,则应使用跳线芯片。这有助于减少分析物谱带展宽的数量。如果需要使用芯片式保护柱(由于分析物基质较脏),则应考虑使用柱温箱升温程序或增加色谱柱内径,以最大程度降低小内径色谱柱可能引起的分析物谱带展宽的影响。
This Technical Overview describes a solution to monitor and trigger the collection of preparative samples with the Agilent 1260 Infinity Evaporative Light Scattering Detector (ELSD) using a flow splitter. The Agilent 1260 Infinity ELSD is the detector of choice to monitor low UV-absorbing compounds or compounds with no UV-chromophore. Furthermore, this detection method is able to detect all compounds less volatile than the mobile phase, suitable for the detection of small molecule mixtures. A drug-like sample mixture has been purified on an Agilent 1260 Infinity Preparative-scale Purification System by triggering the fraction collection with the Agilent 1260 Infinity ELSD. 99 % of purity and 91 % recovery were achieved.