细胞融合--迂回融合腔用于微生物、植物、真菌、酵母及哺乳动物细胞的细胞融合The BTX Meander Fusion Chamber is a microslide with a novel designspecifically used for electro cell fusion. This chamber isconstructed of a conductive metal alloy, which has been depositedin a finger-like projection array on a glass microscope slide. Thismethod of manufacturing creates a highly precise working area. Theconfiguration is designed to give direct viewing of a surface area.This can be used for viewing dimer formation during alignment whileunder a microscope. The gaps are set at 0.2mm.ApplicationsThe Meander Fusion Chamber generates an inhomogenous field and isused for cell fusion of microorganisms, plant, fungi, yeast andmammalian cells. It is compatible with most BTX pulse generators.Cao1 used the Meander Fusion Chamber to generate quadromassecreting bifunctional antibodies. He found this method both rapidand economical to produce several quadromas in a short time.Technical SpecificationsStandard Capabilities *Depending on buffer composition andgenerator capabilityVoltage Range: 0 - 480 VdcFrequency: 1 MHzPulse Length/Time Constants Range: 1 µ sec - 99 msecPulse Number Range: 1-99 (depending on voltage)Operating Temperature: 5° - 40° CIntended Use: Indoor UseRelative Humidity: 20-80%Maximum Altitude: 2000m (6562 ft)Pollution Degree: IIInsulation Category: CAT IPhysical CharacteristicsGap size: 0.2 mmElectrode Material: SilverField Type: InhomogeneousCompatibilityGenerators: ECM 630, 830, 2001, 200, 600, & T820.Ordering InformationModel Description Part Number454 Meander Fusion Chamber, pkg. of 4 01-000098-01465 Square-post Micrograbber Cable 06-700044-015343 Coaxial-Banana Connection Cable 06-700042-014001 Enhancer 400 w/ Computer & Printer 01-001483-01ReferenceCao et al., A rapid non-selective method to generate quadromas bymicroelectrofusion, Journal of Immunological Methods, 187: 1-7(1995). BTX Reference ID#3194.
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