Titansphere TiO 二氧化钛填料 5010-21315
Titansphere TiO 二氧化钛填料 Titansphere TiO填料,是将二氧化钛(TiO2)做为骨架结构的全新型HPLC用球状二氧化钛凝胶填充剂. 是唯一一款产业化了的二氧化钛凝胶色谱填料.以下为二氧化钛色谱填料。Titansphere TiO填料是一种在碱条件下也同样能够使用的,与硅胶有着不同吸附特性的填充剂。其主要特点如下:表面、形状平整的球状二氧化钛凝胶表面积比较大选择性保留磷酸化合物优异的结构异构体分离能力优异的耐酸、耐碱性 磷酸化肽的精制和浓缩细胞中的磷酸化蛋白质,其存在量为微量且离子化效率低,因此质谱(MS)检测比较困难。但是,如果使用以二氧化钛(二氧化钛:TiO2)形成的均一多孔质球状粒子TitansphereTiO,则可从蛋白质酶切物中选择性地浓缩精制磷酸化肽,使MS检测变得容易。操作方法全部为离心操作,几乎没有人为误差。处理时间约40分钟,可在短时间内完成精制? 浓缩。 磷酸化肽选择的精制浓缩珠Titansphere TiO 填料是能够选择性地精制 浓缩磷酸化肽的高性能填料。使用拟南芥细胞提取物,与市售IMAC 进行了比较,磷酸化肽的总峰面积约2.6 倍、肽鉴定数约1.8 倍,可检测到大部分磷酸化肽。 FeaturesSpherically shaped porous particle “Titansphere TiO” (Titanium Dioxide TiO2) is a high-performing bulk material to enrich and purify phosphopeptides selectively.Efficient purification from HeLa Cell LysateThe graph below shows the superior performance of Titansphere TiO using the HeLa Cell Lysate consisting mainly of non-phosphorylated peptides. Titansphere TiO shows exceptional selectivity – almost 90% of the bound peptides were phosphopeptides, and excellent capacity for total phosphopeptide binding. Whereas a competitive TiO product binds mainly non-phosphorylated peptides and a much lower total number of discrete phosphopeptides. Sample: HeLa Cell Lysate, Sample volume: 50 μg, Titansphere TiO volume: 1 mg Comparison of Titansphere TiO and IMACThe graph below shows the comparison of Titansphere TiO and IMAC enrichment using Arabidopsis cell extract. Titansphere TiO provides substantially higher total capacity and a much higher number of isolated phosphopeptides.Sample: Arabidopsis Cell Extract, Sample volume: 100 μg, Titansphere TiO volume: 1 mgExample of how to make a Titanium Column? Cut the Empore Disk C8 and put it at the tip of the 200ul pipette tip to be used as a filter.? Stir and suspend the Titansphere TiO atCH3CN/H2O=80/20.v/v then load it to the tip. (Adjust the amounf of the beads depending on the purpose)? Pressure the tip by a plastic syringe. SpecificationsDescriptionTitansphere TiOParticle Size5 μm, 10 μmParticle ShapeSphericalAdsorption SpotTitanium Dioxide CrystalPore Size100 ? (10 μm)pH Range2 - 12Gravity1.74订货信息Identified Numbers of Phosphopeptides PhosphorylateNon - PhosphorylateTitansphere TiO846198IMAC474379Titansphere™ TiO Part NumberDescriptionVolumeCat.No.Titansphere TiO 5 μm500 mg5020-75000Titansphere TiO 10 μm500 mg5020-75010* Titansphere Phos-TiO Bulk 10 um500 mg5010-21315 * New material for higher purification efficiency of PhosphopeptidesEmpore Disk C8 Part NumberDescriptionQuantityCat.No.Empore Disk C8, 47 mmDIA, 0.5 mm Thickness20 pcs5010-30002Empore Disk C8, 90 mmDIA, 0.5 mm Thickness10 pcs5010-30003