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机械升降摇摆实验淬火槽　　城池牌升降摇摆试验淬火槽是针对实验室环境下模拟苛刻要求情况下的设计专用型淬火槽，根据淬火介质不同，可分为升降淬火油槽，升降淬火水槽，升降淬火盐水槽等。　　本淬火槽由不锈钢槽体、提升系统、搅拌系统、摇摆系统、加热系统、循环冷却系统、各传感系统及控制系统构成。　　升降摇摆试验淬火槽槽体选用不锈钢折弯焊接而成。由于实验室状态下淬火工件较小，重量轻等特点选用简单、可靠、实用的机械升降方式，在满足淬火需求情况下，不仅减轻重量、结构简单、可靠，当做为淬火油槽使用时淬火结束后提升后做沥油用，不仅可以避免淬火油的浪费而且可以使环境更清洁。　　摇摆系统采用往复运动方式，淬火时产生的气泡快速破裂，使得需要淬火的工件快速冷却，从而使晶粒更加细密，使组织由奥氏体转变为马氏体转变的更快更完全，从而提高淬火工件的产品品质。特别适用于钢铁行业。　　城池工业炉生产的机械升降摇摆实验淬火槽全自动化程度高，结实耐用，使用便捷，结构简单，性能可靠，用途多样，深受广大用户信赖爱。欢迎您来电来函咨询！

Acclaim 300 C18 蛋白和肽柱使用 Thermo Scientific™ Acclaim™ 300 C18 液相色谱柱，可进行高分离度肽图分析和蛋白质分离。 3μm 超纯硅胶颗粒可降低分析物进入固定相的扩散限值，从而可快速进行分离度分离。 与 5μm 色谱柱填料相比，较小的颗粒支持在更短的色谱柱中增加流速并采用更浅的梯度，从而加快分离速度。描述高分辨率分离蛋白质和肽 300? 微孔硅胶上的高性能 C18 化学键合经过应用测试，适用于肽图分析重现性好，结果可靠流失率极低，与质谱完全兼容在 pH 值 2.5 - 7.5 之间保持稳定关于 Acclaim HPLC 色谱柱系列选择性互补、柱效高、分离度超高固定相多种多样，包括常规的 C8 和 C18 固定相、极性嵌入键合相和专用键合相（包括大孔径）经过性能测试，可确保高质量和良好的可靠性使用 Thermo Scientific™ Acclaim™ PepMap™ 100 C18 液相色谱柱对胰蛋白酶肽、天然肽和合成肽进行高分辨率肽图分析。 这些色谱柱通常用于蛋白鉴定、生物标记物发现和系统生物学的 LC-MS/MS 肽图分析。 Acclaim PepMap 100 C18 色谱柱载样能力高，因此非常适合分析复杂蛋白组学样品中的低丰度肽。 大多数 C18 色谱柱都预先组装了 Thermo Scientific™ nanoViper™ 手拧接头，可实现轻松安装。 另外也可提供传统色谱柱。描述为蛋白质鉴定、生物标记发现和系统生物学提供高分辨率其特定的载荷能力可在 LC/MS 中实现最高灵敏度设计用于无 TFA 的 LC-MS，最大限度减少离子抑制作用为接入 ESI/MS 和 MALDI-MS 进行过优化柱间重现性最高使用方便，尖端微型化 HPLC预装 nanoViper 柱入口接头，便于安装色谱柱nanoViper 接头的主要优点 使用方便，小号毛细管没有套管组装时不需使用工具不会因过紧而损坏色谱柱：可承受高达 1000 bar 的压力，兼容第三方阀门和两通不会因连接不当而导致试验失败最适合对胰蛋白酶肽、天然肽和合成肽进行高分辨率肽图分析，以及进行关于蛋白质鉴定、生物标记发现和系统生物学的 LC-MS/MS 肽图分析。高载样量用来分析经常存在于复杂蛋白质组学样品中的低丰度肽根据通量、灵敏度和样品可用量，选择色谱柱的长度和内径。可选择长度 50、150、250、500 mm，内径 50、75、300 和 1000 μm卓越的蛋白和多肽分离。不含三氟乙酸 (TFA) 的 LC/MS 为提高 MS 灵敏度，使抑制效应最小化卓越的分离效率卓越的分离度和回收率使用 nanoViper™ 手旋接头轻松安装 PepMap 纳米色谱柱还可预装 Thermo Scientific™ Dionex™ nanoViper™ 手拧接头，以零死体积的状态连接至 1000 bar。无需工具实现几乎零死体积连接承受压力最高可达 1000 bar关于纳米、毛细和微型 Acclaim PepMap 色谱柱纳米 (75 μm)、毛细 (300 μm) 和微型 (1000 μm) 色谱柱适用于蛋白和肽分析。 通常，对样品量有限制，较小内径的色谱柱可以在样品有限的情况下提高灵敏度。 当纳米 LC 色谱柱常用于蛋白组学时，毛细色谱柱和微型色谱柱的浓缩效应对代谢组学或推进 LC-MS 连接有益。 Thermo Scientific Acclaim PepMap 色谱柱在各类针对肽和蛋白分析的化学作用中提供纳米柱、毛细柱和微型柱。 在借助质谱检测功能进行液相色谱分离之前，可使用 PepMap Trap 色谱柱进行肽脱盐和预富集。 捕获柱可为一维肽图分析和二维液相色谱分析提供最高效率。Acclaim 300 C18 蛋白和肽柱订货信息：分析柱Acclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)60263Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)60264Acclaim 300, C18, 3 μm (3.0 x 150 mm)63684Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)60265Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)60266保护柱Acclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 06958069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA69697HardwareAcclaim SST Guard Cartridge Holder V-159456Guard to Analytical Column Coupler59457Guard Kit (Holder and Coupler)59526Acclaim SST Guard Cartridge Holder V-269580Acclaim Guard Kit (Holder and coupler) V-269707Guard to Analytical Column Coupler V-274188Micro and Nano ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper16456775 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164708300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164718Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm163584Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572High-Resolution Separation of Proteins and PeptidesThe Acclaim® 300 features 3 μm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 μm spherical silica substrateHigh-performance bonding chemistry on 300 ? pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 μm silica particle reduces limits for diffusion of the analytes intothe stationary phase, resulting in fast, high-resolution separations. Compared to 5 μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance. Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthCytochrome c tryptic digest. Comparison of acid additives for peptide analysis. Capillary LC for peptide mapping. Protein mixture separated using reversed-phase HPLC.BrochuresCatalog: Acclaim Bonded Silica-Based Columns for HPLCProduct Data SheetsAcclaim 300 HPLC Columns Data SheetApplication NotesAN 1055: Determination of Virginiamycin, Erythromycin, and Penicillin in Dried Distillers Grains with SolublesAN 230: Purity Analysis of Synthetic Thymosin Alpha 1 by Reversed-Phase HPLC with an Acclaim 300 C18 Column Application UpdatesAU 156: Evaluation of Acclaim HPLC Columns Using the National Institute of Standards Standard Reference Material 870AU 183: Separation of Peptides from Enzymatic Digestion on Different Acclaim Columns: A Comparative StudyTechnical NotesTN 705: Automated Enrichment and Determination of Phosphopeptides Using Immobilized Metal Affinity and Reversed-Phase Chromatography with Column SwitchingGuidesAcclaim Care and Handling Instructions

CoolCell® 是由美国BioCision公司开发的一款物理方式实现细胞程序降温的冻存盒，仅需将冻存管样品放入盒内，放在-80℃冰箱中，即可确保标准降温速率-1℃/分钟。这替代了传统异丙醇降温过程中刺激性气味对人体的危害，也降低了实验室污染风险。如今CoolCell细胞程序降温盒已经广泛应用于免疫细胞治疗中的干细胞、初代细胞、PBMC细胞、及各种细胞样本冻存环节，在生物样本库行业广泛使用。CoolCell® LX，需要通过-80℃冰箱提供冷源条件，通过结构上的设计，内置金属蓄冷能量芯，可实现-1℃/分钟，为细胞降温冻存条件提供了非常理想的降温速率条件，使用后在仅需打开盖子很短时间即可恢复至室温条件。CoolCell® FTS30采用同样的原理，在结构上进行了优化设计，可同时进行30支冻存管的降温过程控制，内置金属导热能量芯，及透孔式空气导流设计，确保-1℃/分钟的理想降温速率。

绿百草科技专业提供分析ATP降解产物的色谱柱 Kromasil C18，货号为100-5-C18 4.6 × 250 关键词：Kromasil C18分析柱，100-5-C18 4.6 × 250，ATP降解产物，绿百草科技 绿百草科技专业提供Kromasil C18分析柱。货号为100-5-C18 4.6 × 250的Kromasil C18分析柱可用于分析ATP降解产物。流动相是磷酸盐缓冲溶液（pH 7.00， 60mM 磷酸氢二钾+40 mM 磷酸二氢钾），流速为1mL /min。绿百草科技可提供详细的操作条件和谱图。 需要详细的信息请和绿百草科技联系：010-51659766 登录网站获得更多产品信息: www.greenherbs.com.cN

iCellBox 简 介u BIO-GENER 的 iCellBox 不需要任何液体及添加剂的辅助，只需要结合一个-80℃的冰箱，将提供出-1℃每分的降温速率，是一个非常理想的细胞保存组合。iCellBox 采用径向对称设计配合复合航空泡沫及贵重合金，具有零添加零排放的特点，并且不需要更换任何部件就能永久性使用，并且一直保持着原有性能。真正响应到绿色环保的号召。 图为 12 个有加载 1ml 试管放入 iCellBox 中在 20℃放入-80℃冰箱里连续 5 次的降温曲线 使用方法u 将闲置的 iCellBox 直接存放放于室内，用时直接装入试管后转移到-80℃的冰箱里面，等到用时直接将试管取出注意事项u 请使用与 iCellBox 对应的试管，否则保存结果可能会有偏差u 放入冰箱前请保证金属圈是平躺在盒底的，且盖子是盖住的u 放入冰箱后避免与其他物质紧挨，务必保证周围有 10cm 间隙清洁与保养u 清洁内部可用抹布擦拭，也可以用气枪吹。u 闲置时请置于干燥与阴凉处，尽量避免 60℃以上高温及紫外光线

升降台Go up estalation stage一、概况及用途: 升降台的生产是以钢板镀铬、不锈钢板、电木板等材料，经过冲截成需要的尺寸，进行钻孔、喷膝等工艺最后用螺丝组装而成。它适用于各科研、医疗卫生、院校和工矿企业等单位化验室，在进行化验、滴定分析操作时根据支架各种仪器的特点和操作时需要的高低，用升降台来调整配合仪器的高度，使整个化验、滴定操作较方便的进行。二、使用方法: 取升降台放于化验或滴定操作的支架仪器需要配合的位置上，先旋转升降的螺丝柄将开降台的平面调整到适当的高度、再将需:要配合的仪器置于升降台的平面上，即可进行操作。

升降台Go up estalation stage一、概况及用途: 升降台的生产是以钢板镀铬、不锈钢板、电木板等材料，经过冲截成需要的尺寸，进行钻孔、喷膝等工艺最后用螺丝组装而成。它适用于各科研、医疗卫生、院校和工矿企业等单位化验室，在进行化验、滴定分析操作时根据支架各种仪器的特点和操作时需要的高低，用升降台来调整配合仪器的高度，使整个化验、滴定操作较方便的进行。二、使用方法: 取升降台放于化验或滴定操作的支架仪器需要配合的位置上，先旋转升降的螺丝柄将开降台的平面调整到适当的高度、再将需:要配合的仪器置于升降台的平面上，即可进行操作。

Acclaim PepMap100 C18 蛋白和肽柱使用 Thermo Scientific™ Acclaim™ PepMap™ 100 C18 液相色谱柱对胰蛋白酶肽、天然肽和合成肽进行高分辨率肽图分析。 这些色谱柱通常用于蛋白鉴定、生物标记物发现和系统生物学的 LC-MS/MS 肽图分析。 Acclaim PepMap 100 C18 色谱柱载样能力高，因此非常适合分析复杂蛋白组学样品中的低丰度肽。 大多数 C18 色谱柱都预先组装了 Thermo Scientific™ nanoViper™ 手拧接头，可实现轻松安装。 另外也可提供传统色谱柱。描述为蛋白质鉴定、生物标记发现和系统生物学提供高分辨率其特定的载荷能力可在 LC/MS 中实现最高灵敏度设计用于无 TFA 的 LC-MS，最大限度减少离子抑制作用为接入 ESI/MS 和 MALDI-MS 进行过优化柱间重现性最高 使用方便，尖端微型化 HPLC预装 nanoViper 柱入口接头，便于安装色谱柱nanoViper 接头的主要优点使用方便，小号毛细管没有套管组装时不需使用工具不会因过紧而损坏色谱柱：可承受高达 1000 bar 的压力，兼容第三方阀门和两通不会因连接不当而导致试验失败最适合对胰蛋白酶肽、天然肽和合成肽进行高分辨率肽图分析，以及进行关于蛋白质鉴定、生物标记发现和系统生物学的 LC-MS/MS 肽图分析。 高载样量用来分析经常存在于复杂蛋白质组学样品中的低丰度肽根据通量、灵敏度和样品可用量，选择色谱柱的长度和内径。可选择长度 50、150、250、500 mm，内径 50、75、300 和 1000 μm卓越的蛋白和多肽分离。不含三氟乙酸 (TFA) 的 LC/MS 为提高 MS 灵敏度，使抑制效应最小化卓越的分离效率卓越的分离度和回收率使用 nanoViper™ 手旋接头轻松安装PepMap 纳米色谱柱还可预装 Thermo Scientific™ Dionex™ nanoViper™ 手拧接头，以零死体积的状态连接至 1000 bar。无需工具实现几乎零死体积连接承受压力最高可达 1000 bar关于纳米、毛细和微型 Acclaim PepMap 色谱柱纳米 (75 μm)、毛细 (300 μm) 和微型 (1000 μm) 色谱柱适用于蛋白和肽分析。 通常，对样品量有限制，较小内径的色谱柱可以在样品有限的情况下提高灵敏度。 当纳米 LC 色谱柱常用于蛋白组学时，毛细色谱柱和微型色谱柱的浓缩效应对代谢组学或推进 LC-MS 连接有益。 Thermo Scientific Acclaim PepMap 色谱柱在各类针对肽和蛋白分析的化学作用中提供纳米柱、毛细柱和微型柱。在借助质谱检测功能进行液相色谱分离之前，可使用 PepMap Trap 色谱柱进行肽脱盐和预富集。 捕获柱可为一维肽图分析和二维液相色谱分析提供最高效率。Acclaim PepMap100 C18 蛋白和肽柱订货信息：Nano LC ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper164567Acclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. x 15 cm160321Acclaim PepMap100, C18, 3 μm, 100?, 75 μm i.d. x 25 cm164261Acclaim PepMap100, C18, 5 μm, 100 ?, 75 μm i.d. x 5 cm160318Acclaim PepMap100, C18, 5 μm, 100 ?, 75 μm i.d. x 15 cm160323Acclaim PepMap100, C18, 5 μm, 100 ?, 75 μm i.d. x 25 cm160326Acclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper164567Acclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 15 cm, nanoViper164568AcclaimPepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 25 cm, nanoViper164569Acclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 50 cm, nanoViper164570Capillary LC Columns300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716Acclaim PepMap100, C18, 3 μm, 100 ?, 300 μm i.d. × 15 cm, nanoViper164571Acclaim PepMap100, C18, 5 μm, 100 ?, 300 μm i.d. x 15 cm160297Acclaim PepMap100, C18, 5 μm, 100 ?, 300 μm i.d. x 25 cm160300Acclaim PepMap100, C18, 3 μm, 100 ?, 300 μm i.d. × 15 cm, nanoViper164571Micro LC Columns1.0 mm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164717Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572Acclaim PepMap100, C18, 5 μm, 100 ?, 1.0 mm i.d. x 15 cm160284Acclaim PepMap100, C18, 5 μm, 100 ?, 1.0 mm i.d. x 25 cm160287Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572The Acclaim® PepMap100 C18 column sets the benchmark in peptide separation. The PepMap100 C18 column is available in 3 or 5 μm particle sizes, with 100 ? pore sizes, and in nano, capillary, and micro formats. nanoViper fingertight fittings allow for easy column installation. Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthApplication NotesAN 521: Automated 2D LC Coupled to ESI-MS/MS for the Analysis of Complex Peptide SamplesTechnical NotesTN 60: UltiMate 3000: Direct Sample Injection onto a 75-um I.D. PepMap 100 (C18) ColumnTN 61: UltiMate 3000 — Preconcentration on a 75-μm i.d. x 1 5 cm PepMap 1 00 (C18) Nanocolumn Data SheetsAcclaim PepMap and Acclaim PepMap RSLC Columns for High-Resolution Peptide Mapping Data Sheet

柏丞Cypress塑料沉淀管塑料离心管沉降管、

其它:升降高度280mm承重30kg 升降台Go up estalation stage一、概况及用途: 升降台的生产是以钢板镀铬、不锈钢板、电木板等材料，经过冲截成需要的尺寸，进行钻孔、喷膝等工艺最后用螺丝组装而成。它适用于各科研、医疗卫生、院校和工矿企业等单位化验室，在进行化验、滴定分析操作时根据支架各种仪器的特点和操作时需要的高低，用升降台来调整配合仪器的高度，使整个化验、滴定操作较方便的进行。二、使用方法: 取升降台放于化验或滴定操作的支架仪器需要配合的位置上，先旋转升降的螺丝柄将开降台的平面调整到适当的高度、再将需:要配合的仪器置于升降台的平面上，即可进行操作。

升降台Go up estalation stage一、概况及用途: 升降台的生产是以钢板镀铬、不锈钢板、电木板等材料，经过冲截成需要的尺寸，进行钻孔、喷膝等工艺最后用螺丝组装而成。它适用于各科研、医疗卫生、院校和工矿企业等单位化验室，在进行化验、滴定分析操作时根据支架各种仪器的特点和操作时需要的高低，用升降台来调整配合仪器的高度，使整个化验、滴定操作较方便的进行。二、使用方法: 取升降台放于化验或滴定操作的支架仪器需要配合的位置上，先旋转升降的螺丝柄将开降台的平面调整到适当的高度、再将需:要配合的仪器置于升降台的平面上，即可进行操作。

血浆蛋白结合试验平衡透析装置平衡透析法测定血浆蛋白结合率是用透析膜将蛋白质溶液与缓冲液分隔开，建立在两者之间的一种平衡状态，只有分子量小的药物小分子可以通过。透析的动力是扩散压，扩散压是由横跨膜两边的浓度梯度形成的。透析的速度与膜的厚度、透析的小分子溶质在膜两边的浓度梯度及透析温度等因素有关。平衡透析法能够直接测出为与蛋白结合的药物小分子的数量，这是分析蛋白与小分子物质结合的关键，从而能够求出结合位点数及结合常数。如图所示，利用半透膜将左右两室进行分隔，左侧加入含药的蛋白溶液，右侧加入空白缓冲液，未被结合的游离药物可以自由穿过半透膜，孵育一定时间后两侧达到平衡，游离药物浓度相等，通过测定两侧药物浓度即可计算得到血浆蛋白结合率。平衡透析法在测定药物血浆蛋白结合率具有操作简单、温度易于控制、PH值可调、设备成本低廉等优点。但是同时存在达成平衡时间较长、溶液体积会变化，且透析时间过长可能会造成由加热或代谢引起的被测物质的降解等缺点。因此在利用平衡透析法测定药物血浆蛋白结合率，或者药物与蛋白质相互作用时需要与其他的一些方法联用，才能获得更准确的作用信息。一般只有血浆蛋白结合率高，分布容积小，消除慢以及治疗指数低的药物在临床上的这种相互作用才有意义。3 血浆蛋白结合平衡透析装置 汇智泰康针对血浆蛋白结合率测定试验研发针对性的平衡透析装置，平衡透析装置包括2n个透析池和透析池之间的透析膜组成，可以同时对多个样品进行透析测定，确定游离化合物比例。透析膜（半透膜）选用高分子膜，孔径可根据客户需求定制。汇智泰康可提供产品：平衡透析装置，半透膜，不同种属血浆等。相关产品：血浆蛋白结合平衡透析装置透析膜血浆蛋白结合试剂盒-猴血浆血浆蛋白结合试剂盒-比格犬血浆血浆蛋白结合试剂盒-大鼠血浆血浆蛋白结合试剂盒-小鼠血浆ADME服务项目肝微粒体代谢稳定性（人，猴，犬，大鼠，小鼠）肝细胞代谢稳定性试验（人，猴，犬，大鼠，小鼠）代谢表型研究代谢产物鉴定代谢途径鉴定 种属比较研究CYPP450抑制实验（CYP1A2，CYP2A6，CYP2C9，CYP2C19，CYP2D6，CYP2E1，CYP3A4）CYPP450诱导实验血浆蛋白结合率测定血浆稳定性试验跨膜转运试验药物-药物相互作用毒理学研究ADME相关产品：Ⅰ相代谢稳定性试剂盒Ⅱ相代谢稳定性试剂盒CYP450 酶代谢表型研究试剂盒（化学抑制法/7种抑制剂）CYP450 酶代谢表型研究试剂盒（重组酶法/7种酶）CYP450 酶代谢表型研究试剂盒（重组酶法/单酶）酶抑制（IC50）研究试剂盒（7种特异性底物）酶抑制（IC50）研究试剂盒（单个酶）NADPH再生系统UGT孵育系统0.1M PBS肝微粒体（人，猴，犬，大鼠，小鼠）肝S9（人，猴，犬，大鼠，小鼠）肝原代细胞（人，猴，犬，大鼠，小鼠）CYP450（CYP1A2，CYP2A6，CYP2B6，CYP2C8，CYP2C9，CYP2C19，CYP2D6，CYP2E1，CYP3A4）UGT酶探针底物，代谢产物，抑制剂（CYP1A2，CYP2A6，CYP2B6，CYP2C8，CYP2C9，CYP2C19，CYP2D6，CYP2E1，CYP3A4）汇智泰康是一家位于中美两地的生物医药合同研发机构，整合中美两地的药物研发技术服务平台，基于AAALAC、GLP、ISO/IEC 17025实验室认证，面向全球企业及研发机构提供分析化学、DMPK、药理药效、生物学、以及毒理安全性评价产品与服务。

科研用BD Vacutainer P100血浆蛋白保存系统提取并稳定保存人血浆蛋白-蛋白质组学研究-生物标志物分析

Acclaim PepMap300 C18 蛋白和肽柱使用 Thermo Scientific™ Acclaim™ PepMap™ 100 C18 液相色谱柱对胰蛋白酶肽、天然肽和合成肽进行高分辨率肽图分析。 这些色谱柱通常用于蛋白鉴定、生物标记物发现和系统生物学的 LC-MS/MS 肽图分析。 Acclaim PepMap 100 C18 色谱柱载样能力高，因此非常适合分析复杂蛋白组学样品中的低丰度肽。 大多数 C18 色谱柱都预先组装了 Thermo Scientific™ nanoViper™ 手拧接头，可实现轻松安装。 另外也可提供传统色谱柱。描述为蛋白质鉴定、生物标记发现和系统生物学提供高分辨率其特定的载荷能力可在 LC/MS 中实现最高灵敏度设计用于无 TFA 的 LC-MS，最大限度减少离子抑制作用为接入 ESI/MS 和 MALDI-MS 进行过优化柱间重现性最高使用方便，尖端微型化 HPLC 预装 nanoViper 柱入口接头，便于安装色谱柱nanoViper 接头的主要优点使用方便，小号毛细管没有套管组装时不需使用工具不会因过紧而损坏色谱柱：可承受高达 1000 bar 的压力，兼容第三方阀门和两通不会因连接不当而导致试验失败最适合对胰蛋白酶肽、天然肽和合成肽进行高分辨率肽图分析，以及进行关于蛋白质鉴定、生物标记发现和系统生物学的 LC-MS/MS 肽图分析。 高载样量用来分析经常存在于复杂蛋白质组学样品中的低丰度肽根据通量、灵敏度和样品可用量，选择色谱柱的长度和内径。可选择长度 50、150、250、500 mm，内径 50、75、300 和 1000 μm卓越的蛋白和多肽分离。不含三氟乙酸 (TFA) 的 LC/MS 为提高 MS 灵敏度，使抑制效应最小化卓越的分离效率卓越的分离度和回收率使用 nanoViper™ 手旋接头轻松安装PepMap 纳米色谱柱还可预装 Thermo Scientific™ Dionex™ nanoViper™ 手拧接头，以零死体积的状态连接至 1000 bar。无需工具实现几乎零死体积连接承受压力最高可达 1000 bar关于纳米、毛细和微型Acclaim PepMap 色谱柱纳米 (75 μm)、毛细 (300 μm) 和微型 (1000 μm) 色谱柱适用于蛋白和肽分析。 通常，对样品量有限制，较小内径的色谱柱可以在样品有限的情况下提高灵敏度。 当纳米 LC 色谱柱常用于蛋白组学时，毛细色谱柱和微型色谱柱的浓缩效应对代谢组学或推进 LC-MS 连接有益。 Thermo Scientific Acclaim PepMap 色谱柱在各类针对肽和蛋白分析的化学作用中提供纳米柱、毛细柱和微型柱。在借助质谱检测功能进行液相色谱分离之前，可使用 PepMap Trap 色谱柱进行肽脱盐和预富集。 捕获柱可为一维肽图分析和二维液相色谱分析提供最高效率。Acclaim PepMap300 C18 蛋白和肽柱订货信息：Nano LC ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper164567Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 75 μm x 15 cm163574Capillary LC ColumnsAcclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 300 μm x 5 cm163580300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300A, nanoViper164718Micro LC Columns1.0 mm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300A, nanoViper1647191.0 mm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300A, nanoViper164719Acclaim® PepMap300 C18 is used for the separation of larger peptides and proteins. It is available in 5 μm particle sizes, with 300 ? pore sizes, and in nano, capillary, and micro formats. Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthApplication NotesAN 521: Automated 2D LC Coupled to ESI-MS/MS for the Analysis of Complex Peptide Samples Technical NotesTN 60: UltiMate 3000: Direct Sample Injection onto a 75-um I.D. PepMap 100 (C18) ColumnTN 61: UltiMate 3000 — Preconcentration on a 75-μm i.d. x 1 5 cm PepMap 1 00 (C18) NanocolumnProduct Data SheetsAcclaim PepMap and Acclaim PepMap RSLC Columns for High-Resolution Peptide Mapping Data SheetPepSwift and ProSwift Capillary Monolithic Reversed-Phase Columns Data Sheet

High-Resolution Separation of Proteins and PeptidesThe Acclaim® 300 features 3 μm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 μm spherical silica substrateHigh-performance bonding chemistry on 300 ? pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 μm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthThe standard particle size is nominally 3 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.Analytical ColumnsAcclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)060263Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)060264Acclaim 300, C18, 3 μm (3.0 x 150 mm)063684Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)060265Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)060266Guard ColumnsAcclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 069580069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA069697HardwareAcclaim SST Guard Cartridge Holder V-1059456Guard to Analytical Column Coupler059457Guard Kit (Holder and Coupler)059526Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188 Micro and Nano ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper16456775 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164708300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716 300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164718Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm163584Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572

High-Resolution Separation of Proteins and PeptidesThe Acclaim® 300 features 3 μm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 μm spherical silica substrateHigh-performance bonding chemistry on 300 ? pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 μm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthThe standard particle size is nominally 3 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.Analytical ColumnsAcclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)060263Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)060264Acclaim 300, C18, 3 μm (3.0 x 150 mm)063684Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)060265Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)060266Guard ColumnsAcclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 069580069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA069697HardwareAcclaim SST Guard Cartridge Holder V-1059456Guard to Analytical Column Coupler059457Guard Kit (Holder and Coupler)059526Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188 Micro and Nano ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper16456775 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164708300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716 300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164718Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm163584Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572

High-Resolution Separation of Proteins and PeptidesThe Acclaim® 300 features 3 μm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 μm spherical silica substrateHigh-performance bonding chemistry on 300 ? pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 μm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthThe standard particle size is nominally 3 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.Analytical ColumnsAcclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)060263Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)060264Acclaim 300, C18, 3 μm (3.0 x 150 mm)063684Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)060265Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)060266Guard ColumnsAcclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 069580069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA069697HardwareAcclaim SST Guard Cartridge Holder V-1059456Guard to Analytical Column Coupler059457Guard Kit (Holder and Coupler)059526Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188 Micro and Nano ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper16456775 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164708300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716 300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164718Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm163584Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572

High-Resolution Separation of Proteins and PeptidesThe Acclaim® 300 features 3 μm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 μm spherical silica substrateHigh-performance bonding chemistry on 300 ? pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 μm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthThe standard particle size is nominally 3 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.Analytical ColumnsAcclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)060263Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)060264Acclaim 300, C18, 3 μm (3.0 x 150 mm)063684Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)060265Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)060266Guard ColumnsAcclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 069580069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA069697HardwareAcclaim SST Guard Cartridge Holder V-1059456Guard to Analytical Column Coupler059457Guard Kit (Holder and Coupler)059526Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188 Micro and Nano ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper16456775 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164708300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716 300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164718Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm163584Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572

High-Resolution Separation of Proteins and PeptidesThe Acclaim® 300 features 3 μm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 μm spherical silica substrateHigh-performance bonding chemistry on 300 ? pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 μm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthThe standard particle size is nominally 3 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.Analytical ColumnsAcclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)060263Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)060264Acclaim 300, C18, 3 μm (3.0 x 150 mm)063684Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)060265Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)060266Guard ColumnsAcclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 069580069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA069697HardwareAcclaim SST Guard Cartridge Holder V-1059456Guard to Analytical Column Coupler059457Guard Kit (Holder and Coupler)059526Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188 Micro and Nano ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper16456775 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164708300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716 300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164718Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm163584Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572

High-Resolution Separation of Proteins and PeptidesThe Acclaim® 300 features 3 μm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 μm spherical silica substrateHigh-performance bonding chemistry on 300 ? pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 μm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthThe standard particle size is nominally 3 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.Analytical ColumnsAcclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)060263Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)060264Acclaim 300, C18, 3 μm (3.0 x 150 mm)063684Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)060265Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)060266Guard ColumnsAcclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 069580069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA069697HardwareAcclaim SST Guard Cartridge Holder V-1059456Guard to Analytical Column Coupler059457Guard Kit (Holder and Coupler)059526Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188 Micro and Nano ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper16456775 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164708300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716 300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164718Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm163584Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572

High-Resolution Separation of Proteins and PeptidesThe Acclaim® 300 features 3 μm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 μm spherical silica substrateHigh-performance bonding chemistry on 300 ? pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 μm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthThe standard particle size is nominally 3 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.Analytical ColumnsAcclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)060263Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)060264Acclaim 300, C18, 3 μm (3.0 x 150 mm)063684Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)060265Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)060266Guard ColumnsAcclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 069580069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA069697HardwareAcclaim SST Guard Cartridge Holder V-1059456Guard to Analytical Column Coupler059457Guard Kit (Holder and Coupler)059526Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188 Micro and Nano ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper16456775 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164708300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716 300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164718Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm163584Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572

High-Resolution Separation of Proteins and PeptidesThe Acclaim® 300 features 3 μm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 μm spherical silica substrateHigh-performance bonding chemistry on 300 ? pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 μm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthThe standard particle size is nominally 3 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.Analytical ColumnsAcclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)060263Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)060264Acclaim 300, C18, 3 μm (3.0 x 150 mm)063684Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)060265Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)060266Guard ColumnsAcclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 069580069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA069697HardwareAcclaim SST Guard Cartridge Holder V-1059456Guard to Analytical Column Coupler059457Guard Kit (Holder and Coupler)059526Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188 Micro and Nano ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper16456775 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164708300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716 300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164718Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm163584Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572

High-Resolution Separation of Proteins and PeptidesThe Acclaim® 300 features 3 μm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 μm spherical silica substrateHigh-performance bonding chemistry on 300 ? pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 μm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthThe standard particle size is nominally 3 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.Analytical ColumnsAcclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)060263Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)060264Acclaim 300, C18, 3 μm (3.0 x 150 mm)063684Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)060265Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)060266Guard ColumnsAcclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 069580069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA069697HardwareAcclaim SST Guard Cartridge Holder V-1059456Guard to Analytical Column Coupler059457Guard Kit (Holder and Coupler)059526Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188 Micro and Nano ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper16456775 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164708300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716 300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164718Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm163584Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572

High-Resolution Separation of Proteins and PeptidesThe Acclaim® 300 features 3 μm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 μm spherical silica substrateHigh-performance bonding chemistry on 300 ? pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 μm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthThe standard particle size is nominally 3 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.Analytical ColumnsAcclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)060263Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)060264Acclaim 300, C18, 3 μm (3.0 x 150 mm)063684Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)060265Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)060266Guard ColumnsAcclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 069580069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA069697HardwareAcclaim SST Guard Cartridge Holder V-1059456Guard to Analytical Column Coupler059457Guard Kit (Holder and Coupler)059526Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188 Micro and Nano ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper16456775 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164708300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716 300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164718Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm163584Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572

High-Resolution Separation of Proteins and PeptidesThe Acclaim® 300 features 3 μm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 μm spherical silica substrateHigh-performance bonding chemistry on 300 ? pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 μm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthThe standard particle size is nominally 3 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.Analytical ColumnsAcclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)060263Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)060264Acclaim 300, C18, 3 μm (3.0 x 150 mm)063684Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)060265Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)060266Guard ColumnsAcclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 069580069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA069697HardwareAcclaim SST Guard Cartridge Holder V-1059456Guard to Analytical Column Coupler059457Guard Kit (Holder and Coupler)059526Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188 Micro and Nano ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper16456775 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164708300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716 300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164718Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm163584Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572

High-Resolution Separation of Proteins and PeptidesThe Acclaim® 300 features 3 μm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 μm spherical silica substrateHigh-performance bonding chemistry on 300 ? pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 μm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthThe standard particle size is nominally 3 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.Analytical ColumnsAcclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)060263Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)060264Acclaim 300, C18, 3 μm (3.0 x 150 mm)063684Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)060265Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)060266Guard ColumnsAcclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 069580069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA069697HardwareAcclaim SST Guard Cartridge Holder V-1059456Guard to Analytical Column Coupler059457Guard Kit (Holder and Coupler)059526Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188 Micro and Nano ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper16456775 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164708300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716 300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164718Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm163584Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572

High-Resolution Separation of Proteins and PeptidesThe Acclaim® 300 features 3 μm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 μm spherical silica substrateHigh-performance bonding chemistry on 300 ? pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 μm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthThe standard particle size is nominally 3 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.Analytical ColumnsAcclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)060263Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)060264Acclaim 300, C18, 3 μm (3.0 x 150 mm)063684Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)060265Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)060266Guard ColumnsAcclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 069580069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA069697HardwareAcclaim SST Guard Cartridge Holder V-1059456Guard to Analytical Column Coupler059457Guard Kit (Holder and Coupler)059526Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188 Micro and Nano ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper16456775 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164708300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716 300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164718Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm163584Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572

High-Resolution Separation of Proteins and PeptidesThe Acclaim® 300 features 3 μm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 μm spherical silica substrateHigh-performance bonding chemistry on 300 ? pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 μm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthThe standard particle size is nominally 3 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.Analytical ColumnsAcclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)060263Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)060264Acclaim 300, C18, 3 μm (3.0 x 150 mm)063684Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)060265Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)060266Guard ColumnsAcclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 069580069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA069697HardwareAcclaim SST Guard Cartridge Holder V-1059456Guard to Analytical Column Coupler059457Guard Kit (Holder and Coupler)059526Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188 Micro and Nano ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper16456775 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164708300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716 300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164718Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm163584Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572

High-Resolution Separation of Proteins and PeptidesThe Acclaim® 300 features 3 μm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 μm spherical silica substrateHigh-performance bonding chemistry on 300 ? pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 μm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthThe standard particle size is nominally 3 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.Analytical ColumnsAcclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)060263Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)060264Acclaim 300, C18, 3 μm (3.0 x 150 mm)063684Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)060265Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)060266Guard ColumnsAcclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 069580069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA069697HardwareAcclaim SST Guard Cartridge Holder V-1059456Guard to Analytical Column Coupler059457Guard Kit (Holder and Coupler)059526Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188 Micro and Nano ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper16456775 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164708300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716 300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164718Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm163584Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572

High-Resolution Separation of Proteins and PeptidesThe Acclaim® 300 features 3 μm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 μm spherical silica substrateHigh-performance bonding chemistry on 300 ? pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 μm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 μm300 μm1.0 mmLength50, 150, 250, and 500 mm50, 150, and250 mm50, 150, and250 mmRecommended Flow Rate200 nL/min4 μL/min40 μL/minPacking MaterialC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 100 ?,3 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC18, 300 ?,5 μmC8, 100 ?,3 μm C8, 100 ?,3 μmC8, 100 ?,3 μmC8, 100 ?,5 μm C8, 100 ?,5 μmC8, 100 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmC4, 300 ?,5 μmColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica25-μm i.d./ 280-μm o.d.Fused-silica75-μm i.d./ 280-μm o.d1/16-in.PEEK tubingnanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m LengthThe standard particle size is nominally 3 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.Analytical ColumnsAcclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)060263Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)060264Acclaim 300, C18, 3 μm (3.0 x 150 mm)063684Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)060265Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)060266Guard ColumnsAcclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 069580069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA069697HardwareAcclaim SST Guard Cartridge Holder V-1059456Guard to Analytical Column Coupler059457Guard Kit (Holder and Coupler)059526Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188 Micro and Nano ColumnsAcclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper16456775 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164708300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper164716 300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper164718Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm163584Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper164572