水平式琼脂糖凝胶电泳是基因工程操作中最常规的实验方法,它简单易行,只需少量的DNA就能检测,其分辨效果比分光光度计法与溴化乙啶-标准浓度DNA比较法更高,更直接,检测DNA范围更广,其原理是溴化乙啶在紫外光照射下能发射荧光,当DNA样品在琼脂糖凝胶中电泳时,琼脂糖凝胶中的EB就插入DNA分子中形成荧光络合物;使得DAN发射的荧光,增强几十倍。而荧光的强度正比于DNA的含量,如将已知浓度的标准样品做琼脂糖凝胶电泳的对照,就可比较出待测样品的浓度。若用薄层分析扫描仪检测,则可精确地测得样品的浓度。电泳后的琼脂糖凝胶块直接在紫外光下照射拍照,只需5~10ng DNA ,就可以从照片上比较鉴别。如肉眼观察,可检测到0.01~0.1ng的DNA。
生、烤、脱壳鹰嘴豆粉对无麸质面包工艺及营养特性的影响Impact of Raw, Roasted and Dehulled Chickpea Flours on Technological and Nutritional Characteristics of Gluten-Free Bread.使用格哈特公司蛋白质测定仪和全自动快速脂肪仪检测生、烤、脱壳鹰嘴豆粉无麸质面包蛋白质和脂肪含量
狭叶羽扇豆种子的成分特用于高蛋白品种的筛选Compositional Attributes of Blue Lupin (Lupinus angustifolius) Seeds for Selection of High-Protein CultivarsThe protein content was determined using the combustion method with an automated Dumatherm nitrogen analyzer (C.Gerhardt GmbH & Co. KG, Konigswinter, Germany). Crude fat was determined with an automated SOXTHERM rapid extraction system(C. Gerhardt GmbH & Co. KG, Konigswinter, Germany), using HCl and petroleum ether for fat hydrolysis and extraction.