Dionex AminoPac PA10 氨基酸分析柱
Dionex AminoPac PA10 氨基酸分析柱用途和特点AminoPac PA10柱结合阴离子交换,梯度淋洗以及脉冲电化学检测,可以分离多种氨基酸。分离氨基酸的同时,还可检测氨基糖及碳水化合物。此外,还可分离多种糖类,磷酸化氨基酸,以及含硫氨基酸的普通氧化产物,如磺基丙氨酸。其柱填料为疏水性高聚物薄壳型阴离子交换剂,可在pH0-14范围内使用。填料独特的pH稳定性允许使用的淋洗液,有助于氨基酸在金电极的阳极氧化。Dionex AminoPac PA10 氨基酸分析柱订货信息:分析柱AminoPac PA10 Analytical Column (2 x 250 mm)055406AminoPac PA10 (22 x 250 mm)SP5488AminoPac PA10 (9 x 250 mm)SP5490AminoPac PA10 Analytical Column (4 x 250 mm)SP5678保护柱AminoPac PA10 Guard Column (2 x 50 mm)055407AminoPac PA10 Guard Column (4 x 50 mm)SP5680配件AAA-Direct Installation Kit059539Carbohydrate Removal Cartridge (CRC) for AAA-Direct068598For High-Resolution Separations of Free Amino Acids by AAA-DirectThe AminoPac® PA10 column is packed with a hydrophobic, polymeric, pellicular, anion-exchange resin that is stable over the range of pH 0–14. The unique pH stability allows the use of eluents that are conducive to anodic oxidation of amino acids at gold electrodes. This column is recommended for use with the AAA-Direct™ Amino Acid Analyzer, allowing direct detection of primary and secondary amino acids by IPAD, with no need for pre- or postcolumn derivatization.Derivatization not requiredCarbohydrates and amino acids separated and detected simultaneouslyMid-femtomole to low-picomole detection limitsCompatible with all commonly used hydrolysis proceduresCan be configured for on-line analysisAAA-Direct is a technique that eliminates the need for any form of derivatization, and is therefore compatible with on-line monitoring of cell cultures and fermentation broths. The AminoPac PA10 column is optimized for separations of free amino acids by AAA-Direct. Vitamins, amino sugars, and carbohydrates can be detected simultaneously with amino acids. Additional capabilities include separation of a wide range of sugars, phosphorylated amino acids, and common oxidation products of sulfur-containing amino acids, such as cysteic acid, methionine sulfone, and methionine sulfoxide. AminoPac PA10 Column for Amino Acid Analysis by AAA-Direct SpecificationsRecommended operating temperature30 °CTypical operating conditions0.25 mL/min gradientRecommended flow rate: 0.25 mL/minMobile phase compatibilitypH 0–14 100% compatible with common organic solvents.Dionex sodium acetate ionic form eluents only.Resin composition8.5-μm-diameter microporous (10 ?) bead (55% cross-linked)agglomerated with an 80-nm microbead alkyl ammonium functionalized latex (1% cross-linked).Anion-exchange capacity60 μeq/column (2 × 250 mm analytical column)Column constructionPEEK with 10-32 threaded ferrule-style end fittings.All components are nonmetallic.DimensionsAminoPac PA10 Analytical Column:2 × 250 mmAminoPac PA10 Guard Column:2 × 50 mmMaximum operating pressure4000 psi (27.9 MPa)Determination of amino acids and carbohydrates in LB broth supernatant. Amino acids and sugars in red wine. "High Glucose" Fermentation Broth (1:250 Dilution) Separation of an Amino Acid Standard Mixture Containing 100 pmol of Each Component. Comparison of Amino Acid Concentrations for Collagen Measured by Integrated Amperometry and Ninhydrin Methods.Application NotesAN 179: Carbohydrate and Amino Acid Analysis Using 3-D AmperometryAN 150: Determination of Amino Acids in Cell Cultures and Fermentation BrothsAN 142: Determination of Tryptophan Using AAA-DirectAN 130: Identification of a Hydroxylysine-Containing Peptide Using AAA-DirectTechnical NotesTN 55: Screening of Sample Matrices and Individual Matrix Ingredients for Suitability in AAA-DirectTN 50: Determination of the Amino Acid Content of Peptides by AAA-DirectGuidesAminoPac PA10 Analytical Column AAAAAA Direct System Installation Guide