快速测定动物血浆中的治疗性抗体
• The rapid processing of antibodies using the SMART Digest IA kits allows development of faster methods compared to conventional protein enrichment and digestion by simplifying sample preparation and enabling a reproducible, sensitive, and fully integrated LC/MS/MS workflow.• This application shows a rapid, simple, and sensitive method for the quantification of human IgG in mouse plasma using the SMART Digest IA kits with a total time of 3–4 hours that compares to a process that can take up to 24 hours.• The method described provides a significant simplification for the affinity proteomics process within a single well providing increased sensitivity and reduced sample preparation time.• This Application Note provides a general approach for quantifying low abundance mAbs with reproducible results across a broad dynamic range for all three types of SMART Digest (IA) kits (SMART digest IA Streptavidin, Protein A, and Protein G).