质粒载体

产品特点►载体大小：4.5 kb-11.5 kb►转染方式：直接转染或通过病毒转染►两种标记可选：mCherry/GFP等荧光标记 Puro/Zeocin/Neomycin等药物标记1. CRISPR KI donor载体产品介绍CRISPR KI donor载体是由pUC19质粒改造而来。可直接转染或通过电转导入细胞。该质粒含有eGFP及PuroR筛选基因插入元件，元件两侧含有多个酶切位点，可供插入knock in所需的左右同源臂。经过改造的载体可与spCas9及sgRNA共同作用，达到在特定基因组位点插入含eGFP和Puro抗性基因的目的。本产品通过冰盒运输，到货后请立即放入-20℃冰箱保存。产品参数产品名称CRISPR KI donor 载体载体大小4.5 kb左侧克隆位点Hind Ⅲ、Sph Ⅰ、Sal Ⅰ右侧克隆位点Mlu Ⅰ、Kpn Ⅰ、EcoR Ⅰ产品规格10 μg/支载体示意图2. sgRNA-Puro/mCherry病毒载体等产品介绍CRISPR/Cas9 sgRNA病毒载体是慢病毒系统质粒，可直接转染或通过包装病毒感染细胞。该质粒含有gRNA骨架结构及两种标记以筛选标记基因，客户自行插入靶基因的特定sgRNA序列后，与spCas9共同作用，达到基因编辑的目的。本产品通过冰盒运输，到货后请立即放入-20℃冰箱保存。产品参数产品名称货号载体大小sgRNA克隆位点Esp3 Ⅰ 酶切后片段大小产品规格sgRNA-Puro/mCherry病毒载体GE-20005-PC11.5 kbEsp3 Ⅰ2 kb+9.5 kb10 μg/支sgRNA-mCherry病毒载体GE-20005-C9 kbEsp3 Ⅰ2 kb+7 kb10 μg/支sgRNA-GFP病毒载体GE-20005-G9 kbEsp3 Ⅰ2 kb+7 kb10 μg/支sgRNA-Zeocin病毒载体GE-20005-Z9 kbEsp3 Ⅰ2 kb+7 kb10 μg/支sgRNA-Neomycin病毒载体GE-20005-N9 kbEsp3 Ⅰ2 kb+7 kb10 μg/支sgRNA-Puro病毒载体GE-20005-P9 kbEsp3 Ⅰ2 kb+7 kb10 μg/支sgRNA-Puro/GFP病毒载体GE-20005-PG11.5 kbEsp3 Ⅰ2 kb+9.5 kb10 μg/支使用说明：（请详细阅读使用说明后再开始相关实验） 1.从-20℃冰箱中取出CRISPR/Cas9 sgRNA病毒载体，4℃融化 2.配置酶切反应液 3.37℃，酶切0.5 h 4.终止酶切反应液，将反应液加到1%琼脂糖凝胶孔，跑胶30 min，回收片段，与退火获得的sgRNA靶向序列做连接反应 5.连接产物通过转化获得阳性克隆，测序成功后经过质粒抽提即可使用

产品特点： ASE 萃取池载体篮，用于ASE细胞Carrier Basket （for ASE Cells）订货号：23996产品名称：ASE萃取池的载体篮 （Carrier Basket for ASE Cells）● 坚固的不锈钢结构，可承载完整或空的ASE萃取池和萃取盖。● 可以容纳12个完整的33 mL萃取池组件和更多更小的尺寸。

Resindion公司隶属于三零化工有限公司，通过对旗下耐用的SEPABEADS-EC产品系列进行升级，现推出ReliZyme产品系列，该产品线可用作酶载体，进行酶的固定化。 技术信息： ReliZyme和SEPABEADS-EC，其基质均是刚性的甲基丙烯酸聚合物，但孔隙度不同。具体如下：

G1530-60950Pneumatic carrier assembly气动载体组件

产品编号：780-899 产品中文名称：蜂窝状载体 产品英文名称：Honeycomb Support 产地：美国 适用的仪器：美国LECO分析仪器 供货周期：现货

3D TableTrix® 微载片(微载体)为自主研发产品，由数万颗弹性三维多孔微载体组成，孔隙率90%，粒径大小可控于50-500μm区间， 均一度≤100μm，形成真正的3D仿生培养。 系列产品可用于贴壁细胞的培养并实现细胞、病毒、细胞产物的收获。该产品已获得2项CDE药用辅料资质，登记号为【F20200000496；F20210000003】，1项FDA-DMF药用辅料资质，登记号为【DMF:35481】3D TableTrix® 微载片系列产品，符合GMP相关要求，并获得多项国内外资质&bull 2项CDE药用辅料资质，登记号为【F20200000496；F20210000003】&bull 1项FDA DMF药用辅料资质，登记号为【DMF：35481】 &bull 获得中检院相关残留及安全性评价报告&bull 获得权威第三方安全性评价报告&bull GMP车间规模化生产3D TableTrix® 微载片适用于大多数贴壁细胞的规模化培养并实现细胞、病毒、细胞产物的收获&bull 间充质干细胞的规模化培养及细胞产物的收获&bull 二倍体细胞的规模化培养及病毒、细胞产物的收获&bull VERO、293、MDCK等贴壁细胞的规模化培养及病毒、细胞产物的收获&bull 动物干细胞的规模化培养及细胞收获&bull 其他贴壁细胞的规模化培养及病毒、细胞产物的收获特异性裂解技术，可温和收获细胞&bull 微载体可通过特异性裂解技术，实现细胞100%收获率&bull 微载体残留及裂解残留，已获得中检院残留检测报告及相关安全性评价报告

3D TableTrix® 微载片(微载体)为自主研发产品，由数万颗弹性三维多孔微载体组成，孔隙率90%，粒径大小可控于50-500μm区间， 均一度≤100μm，形成真正的3D仿生培养。 系列产品可用于贴壁细胞的培养并实现细胞、病毒、细胞产物的收获。该产品已获得2项CDE药用辅料资质，登记号为【F20200000496；F20210000003】，1项FDA-DMF药用辅料资质，登记号为【DMF:35481】3D TableTrix® 微载片系列产品，符合GMP相关要求，并获得多项国内外资质&bull 2项CDE药用辅料资质，登记号为【F20200000496；F20210000003】&bull 1项FDA DMF药用辅料资质，登记号为【DMF：35481】&bull 获得中检院相关残留及安全性评价报告&bull 获得权威第三方安全性评价报告&bull GMP车间规模化生产3D TableTrix® 微载片适用于大多数贴壁细胞的规模化培养并实现细胞、病毒、细胞产物的收获&bull 间充质干细胞的规模化培养及细胞产物的收获&bull 二倍体细胞的规模化培养及病毒、细胞产物的收获&bull VERO、293、MDCK等贴壁细胞的规模化培养及病毒、细胞产物的收获&bull 动物干细胞的规模化培养及细胞收获&bull 其他贴壁细胞的规模化培养及病毒、细胞产物的收获特异性裂解技术，可温和收获细胞&bull 微载体可通过特异性裂解技术，实现细胞100%收获率&bull 微载体残留及裂解残留，已获得中检院残留检测报告及相关安全性评价报告

气相色谱填充柱〖GDX-104 高分子多孔微球载体〗部件号描述规格LDPC20246-020GDX-104 高分子多孔微球载体 60-80mesh 填充柱1/8"*2m1. 柱管无特殊说明均为进口不锈钢管，有PEEK管、镍管、惰化管等柱管材料可选2. 采用进口优质填料,填装均匀3. 柱长度可依据客户要求订做4. 色谱柱两端的螺母压环等连接件均可选购，请及时沟通，以免无法连接

产品特点及订货信息： SCOT 不锈钢柱：Supelco is the leader in Support Coated Open Tubular (SCOT) GC column technology. Our unsurpassed manufacturing technique allows us to deposit a uniform layer of liquid phase-coated support particles on the inner wall of stainless steel tubing. This technology gives us access to many phases that are inaccessible to conventional fused silica capillary column manufacturing technology. SCOT columns combine the sensitivity and excellent sample resolution of capillary GC with the extensive stationary phase library of packed GC.All our SCOT columns have dimensions of 50 feet x 1/32 inch O.D. x 0.02 inch I.D. and include 1/16 inch O.D. connections at each end. They are banded in 3.5 inch coils, with 12 inch loose column at each end. Four columns are available as stock items. Columns with other phases may be available through our custom program.Capillary columns are basically of two type wall-coated open tubular (WCOT) and support-coated open tubular (SCOT). WCOT columns consist of a capillary tube, where the walls of the tube are coated with liquid stationary phase. In SCOT capillary columns, the inner wall of the capillary is lined with a thin layer of a particulate support, such as diatomaceous earth onto this support material a thin layer of liquid stationary phase is then coated. This is an added advantage to the SCOT columns as it provides them larger surface area and thicker layer of stationary phase than WCOT columns. material stainless steeldescription 3.5 in. banded coilDimensions: 1/16 in. connectionsSCOT = Support Coated Open TubularBeta value 50application(s) gas chromatography (GC): suitableL × O.D. 50 ft × 1/32 in.matrix active group Squalane phasecolumn type capillary SCOT SCOT 不锈钢柱：* 载体涂布开管柱；* 不锈钢柱；* 高灵敏度；* 良好分辨率；* 多种固定相可选；* 应用：根据固定相不同而不同，特别可分离三种二甲 苯异构体。

产品特点及订货信息： SCOT 不锈钢柱：Supelco is the leader in Support Coated Open Tubular (SCOT) GC column technology. Our unsurpassed manufacturing technique allows us to deposit a uniform layer of liquid phase-coated support particles on the inner wall of stainless steel tubing. This technology gives us access to many phases that are inaccessible to conventional fused silica capillary column manufacturing technology. SCOT columns combine the sensitivity and excellent sample resolution of capillary GC with the extensive stationary phase library of packed GC.All our SCOT columns have dimensions of 50 feet x 1/32 inch O.D. x 0.02 inch I.D. and include 1/16 inch O.D. connections at each end. They are banded in 3.5 inch coils, with 12 inch loose column at each end. Four columns are available as stock items. Columns with other phases may be available through our custom program.Capillary columns are basically of two type wall-coated open tubular (WCOT) and support-coated open tubular (SCOT). WCOT columns consist of a capillary tube, where the walls of the tube are coated with liquid stationary phase. In SCOT capillary columns, the inner wall of the capillary is lined with a thin layer of a particulate support, such as diatomaceous earth onto this support material a thin layer of liquid stationary phase is then coated. This is an added advantage to the SCOT columns as it provides them larger surface area and thicker layer of stationary phase than WCOT columns.material stainless steeldescription 3.5 in. banded coilDNDP = di-n-decyl phthalateDimensions: 1/16 in. connectionsSCOT = Support Coated Open TubularBeta value 45application(s) gas chromatography (GC): suitableL × O.D. 50 ft × 1/32 in.matrix active group Bentone 34/DNDP phasecolumn type capillary SCOT SCOT 不锈钢柱：* 载体涂布开管柱；* 不锈钢柱；* 高灵敏度；* 良好分辨率；* 多种固定相可选；* 应用：根据固定相不同而不同，特别可分离三种二甲 苯异构体。

产品特点及订货信息： SCOT 不锈钢柱：Supelco is the leader in Support Coated Open Tubular (SCOT) GC column technology. Our unsurpassed manufacturing technique allows us to deposit a uniform layer of liquid phase-coated support particles on the inner wall of stainless steel tubing. This technology gives us access to many phases that are inaccessible to conventional fused silica capillary column manufacturing technology. SCOT columns combine the sensitivity and excellent sample resolution of capillary GC with the extensive stationary phase library of packed GC.All our SCOT columns have dimensions of 50 feet x 1/32 inch O.D. x 0.02 inch I.D. and include 1/16 inch O.D. connections at each end. They are banded in 3.5 inch coils, with 12 inch loose column at each end. Four columns are available as stock items. Columns with other phases may be available through our custom program.Capillary columns are basically of two type wall-coated open tubular (WCOT) and support-coated open tubular (SCOT). WCOT columns consist of a capillary tube, where the walls of the tube are coated with liquid stationary phase. In SCOT capillary columns, the inner wall of the capillary is lined with a thin layer of a particulate support, such as diatomaceous earth onto this support material a thin layer of liquid stationary phase is then coated. This is an added advantage to the SCOT columns as it provides them larger surface area and thicker layer of stationary phase than WCOT columns.material stainless steeldescription 3.5 in. banded coilBMEA = bis-methoxyethyladipateDimensions: 1/16 in. connectionsSCOT = Support Coated Open TubularBeta value 40application(s) gas chromatography (GC): suitableL × O.D. 50 ft × 1/32 in.matrix active group BMEA phasecolumn type capillary SCOT SCOT 不锈钢柱：* 载体涂布开管柱；* 不锈钢柱；* 高灵敏度；* 良好分辨率；* 多种固定相可选；* 应用：根据固定相不同而不同，特别可分离三种二甲 苯异构体。

产品特点及订货信息： SCOT 不锈钢柱：Supelco is the leader in Support Coated Open Tubular (SCOT) GC column technology. Our unsurpassed manufacturing technique allows us to deposit a uniform layer of liquid phase-coated support particles on the inner wall of stainless steel tubing. This technology gives us access to many phases that are inaccessible to conventional fused silica capillary column manufacturing technology. SCOT columns combine the sensitivity and excellent sample resolution of capillary GC with the extensive stationary phase library of packed GC.All our SCOT columns have dimensions of 50 feet x 1/32 inch O.D. x 0.02 inch I.D. and include 1/16 inch O.D. connections at each end. They are banded in 3.5 inch coils, with 12 inch loose column at each end. Four columns are available as stock items. Columns with other phases may be available through our custom program.Capillary columns are basically of two type wall-coated open tubular (WCOT) and support-coated open tubular (SCOT). WCOT columns consist of a capillary tube, where the walls of the tube are coated with liquid stationary phase. In SCOT capillary columns, the inner wall of the capillary is lined with a thin layer of a particulate support, such as diatomaceous earth onto this support material a thin layer of liquid stationary phase is then coated. This is an added advantage to the SCOT columns as it provides them larger surface area and thicker layer of stationary phase than WCOT columns. material stainless steeldescription 3.5 in. banded coilDimensions: 1/16 in. connectionsSCOT = Support Coated Open TubularTCEP = 1,2,3-tris(2-cyanoethoxy)propaneBeta value 40application(s) gas chromatography (GC): suitableL × O.D. 50 ft × 1/32 in.matrix active group TCEP phasecolumn type capillary SCOT SCOT 不锈钢柱：* 载体涂布开管柱；* 不锈钢柱；* 高灵敏度；* 良好分辨率；* 多种固定相可选；* 应用：根据固定相不同而不同，特别可分离三种二甲 苯异构体。

* 带有磁性搅拌器用于微载体培养* 球型玻璃搅拌桨提供了轻柔的搅拌方式* 1:1 的顶端比例创造了良好的气体交换条件* 低析硼硅玻璃符合 USP I型和 ASTM E 438 I型标准，A 类要求* 可高压蒸汽灭菌Wheaton Magna Flex 培养瓶内置了一个flex 型球形玻璃搅拌桨，该搅拌桨会从一个固定位置沿着瓶底的凹槽在液面以上旋转。专为微载体培养设计， 搅拌系统增强了搅拌效果，同时轻柔的搅拌方式使培养液充分循环，确保了载体珠的悬浮。该类型的培养瓶都被设计成有1:1 或更大的顶部空间。搅拌桨通过一个可拆卸的不锈钢销子固定，在移动和倾倒时不会对细胞或微载体造成损伤。500mL 和更大的培养瓶可以配套Wheaton 带孔盖( 订货号W240751)技术参数 订货号 容积（mL) 直径× 高度(mm) 顶盖规格 侧壁盖规格 包装数量 356830 125 65×155 51-40045mm 1

奥淇科化致力为科研单位打造一站式采购平台。 在库品规三十余万种，含盖玻璃、试剂、仪器、耗材配件等。 店铺未上架产品请联系客服。

维修部件部件号 :0515-0669SCREW-MACHINE ASSEMBLY M4 X 0.7 30MM-LGScrew, M4 x 30 mm, T-20 (to attachfan to pneumatics carrier)螺丝，M4x30毫米，T-20（连接风扇到气动载体） Power cordsThe 7820A uses detachable power cords.CountriesVACDescriptionPartnumberJapanC15, 15A8120-5342United States 120V120C19, 20A8120-6894JapanC19, 20A8120-6903AustraliaC19, 16A8120-8619Great Britain/HongKong/Singapore/MalaysiaC19, 13A8120-8620EuropeC19, 16A8120-8621ChinaC19, 15A8121-0070Taiwan/South AmericaC19, 20A8120-6360Swizerland, DenmarkC19, 16A8120-8622IsraelC19, 16A8121-0161ArgentinaC19, 16A8121-0675India, South AfricaC19, 15A8121-0710China250C13, 10A8121-0723Korea250C19, 15A8121-1222Thailand220C19, 15A, 1.8M8121-1301Brazil250C198121-1787ItemDescriptionPart numberQty1Inlet fan assemblyG3430-6059012Pneumatics area fanG3430-6000613Screw, M4 x 30 mm, T-20 (to attachfan to pneumatics carrier)0515-06694

技术背景CRISPR/Cas是一套最早在细菌中发现的由RNA引导的DNA内切酶系统。CRISPR/Cas9系统主要由gRNA（guide RNA）和Cas9蛋白两部分组成。针对目的基因，通过人工设计的gRNA来识别目的基因序列，并引导Cas9蛋白酶对特定区域DNA 双链进行有效切割，造成DNA双链的断裂，激起细胞以非同源末端连接或同源重组的方式进行修复，从而实现基因敲除。产品介绍Quick KO® 基因敲除试剂盒是一款专为科研用户定制研发的 all-in-one 即用型CRISPR基因敲除操作试剂盒。其内包含了CRISPR基因敲除所需的，从gRNA设计到获得敲除细胞株，完成实验的重要材料。在完成基因编辑实验的同时，大大提高科研效率。组分Quick-KO® PlasmidQuick-KO® gRNAOptimized SpCas9NC gRNACell Lysis BufffferBuffer ABuffer BValidity TestPCR Master Mix (2×)Control TemplateGenotyping Primer F1Genotyping Primer F2Genotyping Primer R1Genotyping Primer R2ddH2O产品优势1.提供的gRNAs均经过验证，保障敲除成功率 a.每一个出厂的基因敲除试剂盒都经过团队的技术验证，确保敲除效率 b.更高的敲除成功率，避免反复实验，节约实验成本 2.独有载体设计，无需自行构建，到手即用，高效便捷 细胞基因编辑实验流程： a.如已经掌握细胞各项实验参数，可以省去预实验部分，更快完成实验； b.独有载体设计，无需自行构建，提升实验效率； c.只需裂解少量细胞，无需提取和纯化DNA，节约细胞扩增和核酸提取的时间； d.Quick-KO® 采用Multi-gRNA表达策略，在工具细胞中均已验证高效

奥淇科化致力为科研单位打造一站式采购平台。 在库品规三十余万种，含盖玻璃、试剂、仪器、耗材配件等。 店铺未上架产品请联系客服。

奥淇科化致力为科研单位打造一站式采购平台。 在库品规三十余万种，含盖玻璃、试剂、仪器、耗材配件等。 店铺未上架产品请联系客服。

奥淇科化致力为科研单位打造一站式采购平台。 在库品规三十余万种，含盖玻璃、试剂、仪器、耗材配件等。 店铺未上架产品请联系客服。

奥淇科化致力为科研单位打造一站式采购平台。 在库品规三十余万种，含盖玻璃、试剂、仪器、耗材配件等。 店铺未上架产品请联系客服。

奥淇科化致力为科研单位打造一站式采购平台。 在库品规三十余万种，含盖玻璃、试剂、仪器、耗材配件等。 店铺未上架产品请联系客服。

上海安帕特实验室仪器有限公司专业提供实验室分析仪器原装配件及耗材，例如：ICP-AES/OES光谱，ICP-MS质谱，AAS原吸、直读光谱，X荧光光谱，碳硫，氧氮等分析仪器配套使用的各种原装零配件及耗材。包括热电（ThermoFisher），珀金埃尔默（PerkinElmer），力可（Leco），戴安，瓦里安（Varian），安捷伦（Agilent），利曼（Leeman），斯派克（Spectro），岛津（Shimadzu）等品牌各种分析仪器的原装配件及耗材。。 配件编号：780-899 产品名称： 产品规格：780-899 仪器厂商：美国力可/LECO 价格：面议 库存：是

产品信息：该载气导管可用于岛津超净气体过滤器 Shimadzu Super-Clean Gas Filter订购信息：岛津GC 载体导管P/N描 述201-48067-05Carrier Gas Supply Pipe (5 m)

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气相色谱填充柱应用谱图集 http://www.instrument.com.cn/netshow/SH101420/down_208960.htm 玻璃填充柱，用于Agilent Agilent/HP 5880、5890、5987和6890型气相色谱仪，Configuration "A"（用于柱上进样，并适合除TCD以外的所有检测器）

气相色谱填充柱应用谱图集 http://www.instrument.com.cn/netshow/SH101420/down_208960.htm 玻璃填充柱，用于Agilent Agilent/HP 5880、5890、5987和6890型气相色谱仪，Configuration "A"（用于柱上进样，并适合除TCD以外的所有检测器）

气相色谱填充柱应用谱图集 http://www.instrument.com.cn/netshow/SH101420/down_208960.htm 玻璃填充柱，用于Agilent Agilent/HP 5880、5890、5987和6890型气相色谱仪，Configuration "A"（用于柱上进样，并适合除TCD以外的所有检测器）

气相色谱填充柱应用谱图集 http://www.instrument.com.cn/netshow/SH101420/down_208960.htm 玻璃填充柱，用于Agilent Agilent/HP 5880、5890、5987和6890型气相色谱仪，Configuration "A"（用于柱上进样，并适合除TCD以外的所有检测器）

气相色谱填充柱应用谱图集 http://www.instrument.com.cn/netshow/SH101420/down_208960.htm 玻璃填充柱，用于Agilent Agilent/HP 5880、5890、5987和6890型气相色谱仪，Configuration "A"（用于柱上进样，并适合除TCD以外的所有检测器）

质粒提取磁珠试剂盒BeaverBeads™ Mini Plasmid Kit采用超顺磁性磁珠和优化的缓冲液体系，可方便、快捷地从大肠杆菌中提取超螺旋质粒。提取过程采用磁性分离，菌体裂解后不需要通过高速离心去除杂质，有利于实现高通量操作。提取的质粒可用于酶切、测序、PCR等后续操作。 产品名称编号规格包装BeaverBeads™ Mini Plasmid Kit70402-100100 rxnsBeaverBeads™ Mini Plasmid Kit70402-500500 rxns操作简单：菌体裂解后无需离心或抽滤；轻松实现高通量：适用于在96孔板中操作；产物纯度高：A 260/280=1.7~2.0。应用实例Agarose gel analysis of plasmids. Plasmid 1~Plasmid 4 were propagated in DH5α. Plasmid 5~Plasmid 7 were propagated in BL21(DE3) pLysS, TOP10 and JM109, respectively.M: Thermo Scientific GeneRuler 1kb DNA Ladder (# SM0311)A: Plasmid extracted by BeaverBeadsTM Mini Plasmid Kit (without centrifugation)B: Plasmid extracted by BeaverBeadsTM after centrifugationC: Plasmid extracted by spin column after centrifugation purifie如上图所示，采用本产品提取的质粒DNA，纯度及超螺旋的构象都能达到传统竞争产品的水平，由于提取过程中采用磁珠法去除裂解液中的杂质(A)，质粒DNA得率会略低于离心后再提取的样品(B、C)，但整个提取过程避免了高速离心步骤，有利于实现高通量及自动化操作。