妥昔单克隆抗体

Agilent Bio MAb 液相柱 填料担体由硬质球形、高度交联的聚苯乙烯二乙烯基苯（PS/DVB）无孔微球组成 填料表面结合了亲水聚合物层，避免抗体蛋白的非特异性结合 填料的弱阳离子交换固定相层采用了不同工艺，使其比Agilent Bio WCX 柱填料的密度更高 专为单克隆抗体基于带电异构体的分离而设计单克隆抗体的全面表征，包括酸性和碱性亚型的鉴定和监测。Agilent Bio MAb 液相柱填充了专为单克隆抗体基于带电量的高分离度分离而设计的特殊树脂。与水性缓冲液、乙腈/丙酮/甲醇和水混合溶液兼容。常用缓冲液为：磷酸盐、三羟甲基氨基甲烷、MES 和醋酸盐。Bio MAb 柱有1.7、3、5 和10 μm 粒径可供选择，较小的粒径能够提供较高的分离度。订货信息：

产品特点：MAbPac™ SCX-10 RS 单克隆抗体分析柱，5 μm为了高分辨率、高效率地分析单克隆抗体 (mAb) 和相关变异体，请使用 Thermo Scientific™ MAbPac™ SCX-10 RS 单克隆抗体分析柱 (5 μm)。独特的无孔薄膜树脂可提供足以将差异仅为一个带电残基的 mAb 变体分离开来的分辨力。亲水层环绕在聚合物珠周围，可避免其与树脂发生疏水相互作用，从而可使色谱柱达到超高柱效。一种获得专利的接枝型阳离子交换表面可提供 pH 选择性控制功能，从而实现高分离度分离。mAb 变体分析性能卓越对 mAb 变体表现出超高分离度非常适合 mAb 的表征和质控评估特别适合用来进行稳定性研究、mAb 治疗方法开发和高通量筛选无与伦比的柱到柱和批到批的可重现性避免了在稳定性和 QA/QC 测试中对色谱柱变异性的担忧。符合生物制药表征的法规要求在标准规格和 UHPLC 规格中，可选粒径为 3 μm、5 μm 和 10 μm。粒径减小的色谱柱不但可提供与 10 μm 色谱柱相同的分离度，而且还能显著缩短分析时间。MAbPac SCX-10 RSBioRS (Rapid Separation), strong cation exchange column designed for monoclonal antibodies and associated charged variants* UHPLC, high throughput analysis* Specially developed bio-inert PEEK lined stainless steel column hardware * High pressure compatibility* Suitable for operation up to 7,000 psiHigher resolution and throughput of mAb charge variant UHPLC separations can be achieved using the MAbPac SCX-10 RS strong cation-exchange phase with specially developed bio-inert PEEK lined stainless steel column hardware. These columns are designed to be used at higher UHPLC conditions to maximize the resolution of mAb variant separation. Higher pressure compatibility of the column hardware allows use of high flow rates for faster separation.订购信息：MAbPac SCX-10RSParticle Size (μm)FormatLength (mm)2.1mm ID4.6mm ID5UHPLC Column50082675082674150088242085209250082515082673

MAbPac SCX-10 RS 色谱柱BioRS, 用于单克隆抗体和相关变异体的强阳离子交换色谱柱 UHPLC，高通量分析 特殊开发的生物惰性的、PEEK衬里的不锈钢色谱柱管 兼容高压力 可在 7,000 psi 条件下使用使用小粒径的 MAbPac 强阳离子交换色谱柱以及特殊设计生物惰性的 PEEK 衬里的不锈钢柱管，可以实现MAb 变体的高分离度、高通量分析。该色谱柱使用更小粒径的色谱填料以及更长的柱子，提高 MAb 变体的分离度，可以在高达 7000 psi 的压力下进行分析。耐高压的柱管使快速分析变为可能。订货信息：

MAbPac SCX-10 RS 色谱柱BioRS, 用于单克隆抗体和相关变异体的强阳离子交换色谱柱 UHPLC，高通量分析 特殊开发的生物惰性的、PEEK衬里的不锈钢色谱柱管 兼容高压力 可在 7,000 psi 条件下使用使用小粒径的 MAbPac 强阳离子交换色谱柱以及特殊设计生物惰性的 PEEK 衬里的不锈钢柱管，可以实现MAb 变体的高分离度、高通量分析。该色谱柱使用更小粒径的色谱填料以及更长的柱子，提高 MAb 变体的分离度，可以在高达 7000 psi 的压力下进行分析。耐高压的柱管使快速分析变为可能。订货信息：

亲和色谱柱填料, 颗粒大小90 μm,适用于重组蛋白质，单克隆抗体

EGFR单克隆抗体的弱阳离子交换柱TSKgel CM-STAT，货号为21965、21966，粒径分别为7&mu m和10&mu m。TSKgel CM-STAT以无孔聚合物为基体，有3.0*35mm、4.6*100mm两种规格。TSKgel CM-STAT色谱柱机械性能稳定，可用于大多数生物聚合物分析，尤其是抗体的分离。绿百草科技可提供TSKgel CM-STAT分析抗EGFR单克隆抗体的操作条件和谱图。 货号 柱名称 官能团 类型 粒径 尺寸mm 21960 TSK gel Q-STAT 季铵盐 阴离子 10 3*35 21961 TSK gel Q-STAT 季铵盐 阴离子 7 4.6*100 21962 TSKgel DNA-STAT 季铵盐 阴离子 5 4.6*100 21963 TSK gel SP-STAT 磺酸基 强阳 10 3*35 21964 TSK gel SP-STAT 磺酸基 强阳 7 4.6*100 21965 TSK gel CM-STAT 羧酸基 弱阳 10 3*35 21966 TSK gel CM-STAT 羧酸基 弱阳 7 4.6*100

绿百草科技专业提供色谱柱YMC-BioPro QA-F和YMC-Pack Diol200色谱柱分离单克隆抗体 关键词：YMC-BioPro QA-F，YMC-Pack Diol200，单克隆抗体，绿百草科技 绿百草科技专业提供YMC-BioPro QA-F离子交换色谱柱和YMC-Pack Diol-200凝胶柱。YMC-BioPro QA离子交换色谱柱可使用离子交换模式分离抗人体单克隆抗体；YMC-Pack Diol-300+Diol-200凝胶柱可使用体积排除（SEC）模式分离抗人体单克隆抗体。 需要详细的信息请和绿百草科技联系：010-51659766 登录网站获得更多产品信息:www.greenherbs.com.cn

Extrem A Diamond是博格隆自主研发的新一代高耐碱Protein A抗体亲和介质。蛋白A配基是在大肠杆菌中表达，与上一代产品AT Protein A Diamond Plus相比，耐碱性更强（可耐受0.5~1.0 M NaOH 的CIP）、载量更高。在工艺放大过程可以选择更小的柱体积来降低生产成本。适合从大批量细胞培养液中捕获单克隆抗体或者Fc融合蛋白，也适合于从腹水或者血浆中捕获多克隆抗体。该介质具有以下特点： 相同接触时间的情况下，较常规产品具有更高的动态结合能力。 蛋白配基的高耐碱性，可在清洗过程耐受0.5~1.0 M NaOH 的CIP。 载量高，能降低生产成本，提高生产效率。

AT Protein A Diamond Plus是一种新型的由耐碱Protein A同高刚性琼脂糖基架通过环氧化学方式合成的亲和介质，优化的介质孔径有利于提高抗体的结合载量，该介质适合从大批量细胞培养液中捕获单克隆抗体或者Fc融合蛋白，也适合于从腹水或者血浆中捕获多克隆抗体。 AT Protein A Diamond Plus的配基为基因工程重组的蛋白A片段，在上游构建的时候采用了耐碱的氨基酸替换了不耐碱的氨基酸，并且替换了蛋白酶敏感的氨基酸，使得该配基具有良好的碱稳定性，配基发酵及后续的纯化过程均采用无任何动物源原材料。该介质可以用0.1~0.5M NaOH进行在位清洗，避免了使用昂贵且具有腐蚀性的在位清洗试剂，可有效节省成本，同时和AT Protein A Diamond相比载量更高，在工艺放大过程可以选择更小的柱体积来降低生产成本。 该介质具有以下特点： 硬度高、反压低、流速快，适合大规模生产应用。 载量高，能降低生产成本。 耐受碱清洗，使用寿命长。

AT Protein A Diamond是一种由耐碱Protein A同高刚性琼脂糖基架通过环氧活化方式偶联后制成的一种新型亲和层析介质，与传统的抗体亲和介质相比，配基更稳定，流速更快，反压更低，适用于从大批量细胞培养液中捕获单克隆抗体或者Fc融合蛋白，也适合于从腹水或者血浆中捕获多克隆抗体。 AT Protein A Diamond的配基为大肠杆菌发酵获得的重组蛋白A片段，在上游构建的时候采用耐碱的氨基酸替换了不耐碱的氨基酸，并且去除了蛋白酶敏感的氨基酸，使得该配基具有良好的碱稳定性，配基发酵及后续的纯化过程均采用无任何动物源原材料。该介质可以采用0.1~0.5M NaOH进行在位清洗，避免了使用昂贵且具有腐蚀性的清洗试剂，可有效节省成本，同时易于进行工业化大规模生产使用。

绿百草科技专业提供分析抗EGFR单克隆抗体的弱阳离子交换柱TSKgel CM-STAT，货号为21965、21966，粒径分别为7&mu m和10&mu m。TSKgel CM-STAT以无孔聚合物为基体，有3.0*35mm、4.6*100mm两种规格。TSKgel CM-STAT色谱柱机械性能稳定，可用于大多数生物聚合物分析，尤其是抗体的分离。绿百草科技可提供TSKgel CM-STAT分析抗EGFR单克隆抗体的操作条件和谱图。

Novo-A Diamond是博格隆自主研发的新一代高耐碱Protein A抗体亲和介质。蛋白A配基是在大肠杆菌中表达，与上一代产品AT Protein A Diamond Plus相比，耐碱性更强，（可耐受0.5~1.0 M NaOH 的CIP）、压力流速性能更好。适合从大批量细胞培养液中捕获单克隆抗体或者Fc融合蛋白，也适合于从腹水或者血浆中捕获多克隆抗体。该介质具有以下特点： 相同接触时间的情况下，较常规产品具有更高的动态结合能力。 蛋白配基的高耐碱性，可在清洗过程耐受0.5~1.0 M NaOH 的CIP。 载量高，能降低生产成本，提高生产效率。

AT Protein A Diamond Ultra是一种新型的由耐碱Protein A同高刚性琼脂糖基架通过环氧化学方式合成的亲和介质，优化的介质孔径有利于提高抗体的结合载量，该介质适合从大批量细胞培养液中捕获单克隆抗体或者Fc融合蛋白，也适合于从腹水或者血浆中捕获多克隆抗体。 AT Protein A Diamond Ultra的配基为基因工程重组的蛋白A片段，在上游构建的时候采用了耐碱的氨基酸替换了不耐碱的氨基酸，并且替换了蛋白酶敏感的氨基酸，使得该配基具有良好的碱稳定性，配基发酵及后续的纯化过程均采用无任何动物源原材料。该介质可以用0.1~0.5M NaOH进行在位清洗，避免了使用昂贵且具有腐蚀性的在位清洗试剂，可有效节省成本，同时和AT Protein A Diamond Plus相比载量更高，在工艺放大过程可以选择更小的柱体积来降低生产成本。 该介质具有以下特点： 硬度高、反压低、流速快，适合大规模生产应用。 载量高，能降低生产成本。 耐受碱清洗，使用寿命长。

MAbPac SCX-10 Monoclonal Antibody Analysis ColumnMAbPac SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of monoclonal antibody (MAb) variant samples.The MAbPac SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of MAbs and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of MAb variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.Features:? Unequalled high-resolution separations? Exceptionally high efficiency? Highest resolution of MAb variants? Unmatched column-to-column and lot-to-lot reproducibility? Elimination of hydrophobic interactions? Ideal for stability testingThe MAbPac resin technology is the basis for the superior performance of MAb variant analysis. The nonporous core particle provides high rates of mass transfer, which results in high-efficiency separations. A hydrophilic layer surrounds the polymeric beads, eliminating hydrophobic interactions between proteins and the resin while contributing to high-efficiency peaks. A proprietary grafted cation-exchange surface provides pH selectivity control, resulting in high-resolution separations. In addition, the MAbPac SCX-10 line of columns provides consistent column-to-column and lot-to-lot reproducibility for MAb variant separation results and is ideal for MAb therapeutic method development and high-throughput screening. The MAbPac SCX-10 RS columns are designed for use with the Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS system. These columns are packed in bioinert, PEEK-lined, stainless steel column hardware suitable for operation up to 10,000 psi. These columns are higher efficiency, and therefore provide higher-resolution separations.MAbPac SCX-10 columns are designed to address the regulatory requirements for biopharmaceutical characterization. The consistent manufacturing processes ensure reproducibility in methods development and data analysis.The MAbPac SCX-10 column is complimentary to the industry-leading Thermo Scientific™ ProPac™ WCX-10 column for MAb variant analysis. The MAbPac SCX-10 column offers alternative selectivity and provides higher resolution and efficiency for variant analysis of most MAb samples than the ProPac WCX-10 column. New MAbPac SCX Column Kits for Validation Now AvailableThree MAbPac SCX-10, 4 × 250 mm columns from one lot of resinThree MAbPac SCX-10, 4 × 250 mm columns, one each from three lots of resinThese MAbPac SCX-10 column kits will facilitate the validation process of MAbPac SCX-10 columns for MAb therapeutics development projects.The MAbPac SCX-10, 10 μm, 9 × 250 mm column is now available for semipreparative applications of MAb variant separations.Fast High-Resolution Analysis of MAb variants achieved in only 15 minutes!MAbPac SCX-10 Monoclonal Antibody Analysis Column SpecificationsColumn Dimensions10 μm: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm, 2 × 250 mm5 μm Analytical: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5 μm Analytical RS: 4.6 × 250 mm, 4.6 × 150 mm, 4.6 x 50mm, 2.1 x 250mm, 2.1 x 150mm, 2.1 x 250mm3 μm: 4 × 50 mmBead SizeUniform 10 μm, 5 μm, 3 μm particlesSubstrateHighly crosslinked DVB mediaPellicular LayerProprietary hydrophilicFunctional GroupSulfonicFlow Rate Range:0.2 to 2 mL/min for: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm0.1 to 0.5 ml/min for: 2 × 250 mmPressure Limit3,000 psi for 10 μm columns: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5,000 psi for Analytical 3 μm and 5 μm columns7,000 psi for Analytical RS 5 μm columnsTemperature Range:Ambient to 60 oCpH Range2–12Typical buffers:Sodium or potassium salts of phosphate, MESSolvents:50% acetonitrile if needed for cleaningShort Term Storage Solution (overnight):Your low salt concentration eluent (pH 3–10)Shipping Solution / Long Term Storage Solution:20 mM MES (pH 5.6) + 60 mM NaCl– + 0.1% sodium azideDetergent CompatibilityNonionic, anionic, or zwitterionic detergents. MAbPac SCX-10 RS ColumnsMAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 50 mm)082674MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 150 mm)085209MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 250 mm)082673MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 50mm)082675MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 150mm)088242MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 250mm)082515Analytical ColumnsMAbPac SCX-10, 3μm, Analytical Column (4 x 50 mm)077907MAbPac SCX-10, 5μm, Analytical Column (4 x 50 mm)078656MAbPac SCX-10, 5μm, Analytical Column (4 x 150 mm)085198MAbPac SCX-10, 5μm, Analytical Column (4 x 250 mm)078655MAbPac SCX-10, 10 μm, Analytical Column (4 x 250mm)074625MAbPac SCX-10, 10μm, Analytical Column (4 x 150mm)075602MAbPac SCX-10HT, 10μm, Analytical Column (4 x 50mm)075603MAbPac SCX-10, 10μm, Analytical Column (2 x 250mm)075604MAbPac SCX-10, 10μm, 9 x 250 MM columnSP6866MAbPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)SP6864MAPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)SP6865Guard ColumnsMAbPac SCX-10G, 10μm, Guard Column (4 x 50 mm)074631MAbPac SCX-10G, 10μm, Guard Column (2 x 50 mm)075749

MAbPac SCX-10 Monoclonal Antibody Analysis ColumnMAbPac SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of monoclonal antibody (MAb) variant samples.The MAbPac SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of MAbs and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of MAb variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.Features:? Unequalled high-resolution separations? Exceptionally high efficiency? Highest resolution of MAb variants? Unmatched column-to-column and lot-to-lot reproducibility? Elimination of hydrophobic interactions? Ideal for stability testingThe MAbPac resin technology is the basis for the superior performance of MAb variant analysis. The nonporous core particle provides high rates of mass transfer, which results in high-efficiency separations. A hydrophilic layer surrounds the polymeric beads, eliminating hydrophobic interactions between proteins and the resin while contributing to high-efficiency peaks. A proprietary grafted cation-exchange surface provides pH selectivity control, resulting in high-resolution separations. In addition, the MAbPac SCX-10 line of columns provides consistent column-to-column and lot-to-lot reproducibility for MAb variant separation results and is ideal for MAb therapeutic method development and high-throughput screening. The MAbPac SCX-10 RS columns are designed for use with the Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS system. These columns are packed in bioinert, PEEK-lined, stainless steel column hardware suitable for operation up to 10,000 psi. These columns are higher efficiency, and therefore provide higher-resolution separations.MAbPac SCX-10 columns are designed to address the regulatory requirements for biopharmaceutical characterization. The consistent manufacturing processes ensure reproducibility in methods development and data analysis.The MAbPac SCX-10 column is complimentary to the industry-leading Thermo Scientific™ ProPac™ WCX-10 column for MAb variant analysis. The MAbPac SCX-10 column offers alternative selectivity and provides higher resolution and efficiency for variant analysis of most MAb samples than the ProPac WCX-10 column. New MAbPac SCX Column Kits for Validation Now AvailableThree MAbPac SCX-10, 4 × 250 mm columns from one lot of resinThree MAbPac SCX-10, 4 × 250 mm columns, one each from three lots of resinThese MAbPac SCX-10 column kits will facilitate the validation process of MAbPac SCX-10 columns for MAb therapeutics development projects.The MAbPac SCX-10, 10 μm, 9 × 250 mm column is now available for semipreparative applications of MAb variant separations.Fast High-Resolution Analysis of MAb variants achieved in only 15 minutes!MAbPac SCX-10 Monoclonal Antibody Analysis Column SpecificationsColumn Dimensions10 μm: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm, 2 × 250 mm5 μm Analytical: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5 μm Analytical RS: 4.6 × 250 mm, 4.6 × 150 mm, 4.6 x 50mm, 2.1 x 250mm, 2.1 x 150mm, 2.1 x 250mm3 μm: 4 × 50 mmBead SizeUniform 10 μm, 5 μm, 3 μm particlesSubstrateHighly crosslinked DVB mediaPellicular LayerProprietary hydrophilicFunctional GroupSulfonicFlow Rate Range:0.2 to 2 mL/min for: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm0.1 to 0.5 ml/min for: 2 × 250 mmPressure Limit3,000 psi for 10 μm columns: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5,000 psi for Analytical 3 μm and 5 μm columns7,000 psi for Analytical RS 5 μm columnsTemperature Range:Ambient to 60 oCpH Range2–12Typical buffers:Sodium or potassium salts of phosphate, MESSolvents:50% acetonitrile if needed for cleaningShort Term Storage Solution (overnight):Your low salt concentration eluent (pH 3–10)Shipping Solution / Long Term Storage Solution:20 mM MES (pH 5.6) + 60 mM NaCl– + 0.1% sodium azideDetergent CompatibilityNonionic, anionic, or zwitterionic detergents. MAbPac SCX-10 RS ColumnsMAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 50 mm)082674MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 150 mm)085209MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 250 mm)082673MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 50mm)082675MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 150mm)088242MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 250mm)082515Analytical ColumnsMAbPac SCX-10, 3μm, Analytical Column (4 x 50 mm)077907MAbPac SCX-10, 5μm, Analytical Column (4 x 50 mm)078656MAbPac SCX-10, 5μm, Analytical Column (4 x 150 mm)085198MAbPac SCX-10, 5μm, Analytical Column (4 x 250 mm)078655MAbPac SCX-10, 10 μm, Analytical Column (4 x 250mm)074625MAbPac SCX-10, 10μm, Analytical Column (4 x 150mm)075602MAbPac SCX-10HT, 10μm, Analytical Column (4 x 50mm)075603MAbPac SCX-10, 10μm, Analytical Column (2 x 250mm)075604MAbPac SCX-10, 10μm, 9 x 250 MM columnSP6866MAbPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)SP6864MAPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)SP6865Guard ColumnsMAbPac SCX-10G, 10μm, Guard Column (4 x 50 mm)074631MAbPac SCX-10G, 10μm, Guard Column (2 x 50 mm)075749

MAbPac SCX-10 Monoclonal Antibody Analysis ColumnMAbPac SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of monoclonal antibody (MAb) variant samples.The MAbPac SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of MAbs and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of MAb variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.Features:? Unequalled high-resolution separations? Exceptionally high efficiency? Highest resolution of MAb variants? Unmatched column-to-column and lot-to-lot reproducibility? Elimination of hydrophobic interactions? Ideal for stability testingThe MAbPac resin technology is the basis for the superior performance of MAb variant analysis. The nonporous core particle provides high rates of mass transfer, which results in high-efficiency separations. A hydrophilic layer surrounds the polymeric beads, eliminating hydrophobic interactions between proteins and the resin while contributing to high-efficiency peaks. A proprietary grafted cation-exchange surface provides pH selectivity control, resulting in high-resolution separations. In addition, the MAbPac SCX-10 line of columns provides consistent column-to-column and lot-to-lot reproducibility for MAb variant separation results and is ideal for MAb therapeutic method development and high-throughput screening. The MAbPac SCX-10 RS columns are designed for use with the Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS system. These columns are packed in bioinert, PEEK-lined, stainless steel column hardware suitable for operation up to 10,000 psi. These columns are higher efficiency, and therefore provide higher-resolution separations.MAbPac SCX-10 columns are designed to address the regulatory requirements for biopharmaceutical characterization. The consistent manufacturing processes ensure reproducibility in methods development and data analysis.The MAbPac SCX-10 column is complimentary to the industry-leading Thermo Scientific™ ProPac™ WCX-10 column for MAb variant analysis. The MAbPac SCX-10 column offers alternative selectivity and provides higher resolution and efficiency for variant analysis of most MAb samples than the ProPac WCX-10 column. New MAbPac SCX Column Kits for Validation Now AvailableThree MAbPac SCX-10, 4 × 250 mm columns from one lot of resinThree MAbPac SCX-10, 4 × 250 mm columns, one each from three lots of resinThese MAbPac SCX-10 column kits will facilitate the validation process of MAbPac SCX-10 columns for MAb therapeutics development projects.The MAbPac SCX-10, 10 μm, 9 × 250 mm column is now available for semipreparative applications of MAb variant separations.Fast High-Resolution Analysis of MAb variants achieved in only 15 minutes!MAbPac SCX-10 Monoclonal Antibody Analysis Column SpecificationsColumn Dimensions10 μm: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm, 2 × 250 mm5 μm Analytical: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5 μm Analytical RS: 4.6 × 250 mm, 4.6 × 150 mm, 4.6 x 50mm, 2.1 x 250mm, 2.1 x 150mm, 2.1 x 250mm3 μm: 4 × 50 mmBead SizeUniform 10 μm, 5 μm, 3 μm particlesSubstrateHighly crosslinked DVB mediaPellicular LayerProprietary hydrophilicFunctional GroupSulfonicFlow Rate Range:0.2 to 2 mL/min for: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm0.1 to 0.5 ml/min for: 2 × 250 mmPressure Limit3,000 psi for 10 μm columns: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5,000 psi for Analytical 3 μm and 5 μm columns7,000 psi for Analytical RS 5 μm columnsTemperature Range:Ambient to 60 oCpH Range2–12Typical buffers:Sodium or potassium salts of phosphate, MESSolvents:50% acetonitrile if needed for cleaningShort Term Storage Solution (overnight):Your low salt concentration eluent (pH 3–10)Shipping Solution / Long Term Storage Solution:20 mM MES (pH 5.6) + 60 mM NaCl– + 0.1% sodium azideDetergent CompatibilityNonionic, anionic, or zwitterionic detergents. MAbPac SCX-10 RS ColumnsMAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 50 mm)082674MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 150 mm)085209MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 250 mm)082673MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 50mm)082675MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 150mm)088242MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 250mm)082515Analytical ColumnsMAbPac SCX-10, 3μm, Analytical Column (4 x 50 mm)077907MAbPac SCX-10, 5μm, Analytical Column (4 x 50 mm)078656MAbPac SCX-10, 5μm, Analytical Column (4 x 150 mm)085198MAbPac SCX-10, 5μm, Analytical Column (4 x 250 mm)078655MAbPac SCX-10, 10 μm, Analytical Column (4 x 250mm)074625MAbPac SCX-10, 10μm, Analytical Column (4 x 150mm)075602MAbPac SCX-10HT, 10μm, Analytical Column (4 x 50mm)075603MAbPac SCX-10, 10μm, Analytical Column (2 x 250mm)075604MAbPac SCX-10, 10μm, 9 x 250 MM columnSP6866MAbPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)SP6864MAPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)SP6865Guard ColumnsMAbPac SCX-10G, 10μm, Guard Column (4 x 50 mm)074631MAbPac SCX-10G, 10μm, Guard Column (2 x 50 mm)075749

MAbPac SCX-10 Monoclonal Antibody Analysis ColumnMAbPac SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of monoclonal antibody (MAb) variant samples.The MAbPac SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of MAbs and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of MAb variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.Features:? Unequalled high-resolution separations? Exceptionally high efficiency? Highest resolution of MAb variants? Unmatched column-to-column and lot-to-lot reproducibility? Elimination of hydrophobic interactions? Ideal for stability testingThe MAbPac resin technology is the basis for the superior performance of MAb variant analysis. The nonporous core particle provides high rates of mass transfer, which results in high-efficiency separations. A hydrophilic layer surrounds the polymeric beads, eliminating hydrophobic interactions between proteins and the resin while contributing to high-efficiency peaks. A proprietary grafted cation-exchange surface provides pH selectivity control, resulting in high-resolution separations. In addition, the MAbPac SCX-10 line of columns provides consistent column-to-column and lot-to-lot reproducibility for MAb variant separation results and is ideal for MAb therapeutic method development and high-throughput screening. The MAbPac SCX-10 RS columns are designed for use with the Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS system. These columns are packed in bioinert, PEEK-lined, stainless steel column hardware suitable for operation up to 10,000 psi. These columns are higher efficiency, and therefore provide higher-resolution separations.MAbPac SCX-10 columns are designed to address the regulatory requirements for biopharmaceutical characterization. The consistent manufacturing processes ensure reproducibility in methods development and data analysis.The MAbPac SCX-10 column is complimentary to the industry-leading Thermo Scientific™ ProPac™ WCX-10 column for MAb variant analysis. The MAbPac SCX-10 column offers alternative selectivity and provides higher resolution and efficiency for variant analysis of most MAb samples than the ProPac WCX-10 column. New MAbPac SCX Column Kits for Validation Now AvailableThree MAbPac SCX-10, 4 × 250 mm columns from one lot of resinThree MAbPac SCX-10, 4 × 250 mm columns, one each from three lots of resinThese MAbPac SCX-10 column kits will facilitate the validation process of MAbPac SCX-10 columns for MAb therapeutics development projects.The MAbPac SCX-10, 10 μm, 9 × 250 mm column is now available for semipreparative applications of MAb variant separations.Fast High-Resolution Analysis of MAb variants achieved in only 15 minutes!MAbPac SCX-10 Monoclonal Antibody Analysis Column SpecificationsColumn Dimensions10 μm: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm, 2 × 250 mm5 μm Analytical: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5 μm Analytical RS: 4.6 × 250 mm, 4.6 × 150 mm, 4.6 x 50mm, 2.1 x 250mm, 2.1 x 150mm, 2.1 x 250mm3 μm: 4 × 50 mmBead SizeUniform 10 μm, 5 μm, 3 μm particlesSubstrateHighly crosslinked DVB mediaPellicular LayerProprietary hydrophilicFunctional GroupSulfonicFlow Rate Range:0.2 to 2 mL/min for: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm0.1 to 0.5 ml/min for: 2 × 250 mmPressure Limit3,000 psi for 10 μm columns: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5,000 psi for Analytical 3 μm and 5 μm columns7,000 psi for Analytical RS 5 μm columnsTemperature Range:Ambient to 60 oCpH Range2–12Typical buffers:Sodium or potassium salts of phosphate, MESSolvents:50% acetonitrile if needed for cleaningShort Term Storage Solution (overnight):Your low salt concentration eluent (pH 3–10)Shipping Solution / Long Term Storage Solution:20 mM MES (pH 5.6) + 60 mM NaCl– + 0.1% sodium azideDetergent CompatibilityNonionic, anionic, or zwitterionic detergents. MAbPac SCX-10 RS ColumnsMAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 50 mm)082674MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 150 mm)085209MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 250 mm)082673MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 50mm)082675MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 150mm)088242MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 250mm)082515Analytical ColumnsMAbPac SCX-10, 3μm, Analytical Column (4 x 50 mm)077907MAbPac SCX-10, 5μm, Analytical Column (4 x 50 mm)078656MAbPac SCX-10, 5μm, Analytical Column (4 x 150 mm)085198MAbPac SCX-10, 5μm, Analytical Column (4 x 250 mm)078655MAbPac SCX-10, 10 μm, Analytical Column (4 x 250mm)074625MAbPac SCX-10, 10μm, Analytical Column (4 x 150mm)075602MAbPac SCX-10HT, 10μm, Analytical Column (4 x 50mm)075603MAbPac SCX-10, 10μm, Analytical Column (2 x 250mm)075604MAbPac SCX-10, 10μm, 9 x 250 MM columnSP6866MAbPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)SP6864MAPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)SP6865Guard ColumnsMAbPac SCX-10G, 10μm, Guard Column (4 x 50 mm)074631MAbPac SCX-10G, 10μm, Guard Column (2 x 50 mm)075749

MAbPac SCX-10 Monoclonal Antibody Analysis ColumnMAbPac SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of monoclonal antibody (MAb) variant samples.The MAbPac SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of MAbs and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of MAb variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.Features:? Unequalled high-resolution separations? Exceptionally high efficiency? Highest resolution of MAb variants? Unmatched column-to-column and lot-to-lot reproducibility? Elimination of hydrophobic interactions? Ideal for stability testingThe MAbPac resin technology is the basis for the superior performance of MAb variant analysis. The nonporous core particle provides high rates of mass transfer, which results in high-efficiency separations. A hydrophilic layer surrounds the polymeric beads, eliminating hydrophobic interactions between proteins and the resin while contributing to high-efficiency peaks. A proprietary grafted cation-exchange surface provides pH selectivity control, resulting in high-resolution separations. In addition, the MAbPac SCX-10 line of columns provides consistent column-to-column and lot-to-lot reproducibility for MAb variant separation results and is ideal for MAb therapeutic method development and high-throughput screening. The MAbPac SCX-10 RS columns are designed for use with the Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS system. These columns are packed in bioinert, PEEK-lined, stainless steel column hardware suitable for operation up to 10,000 psi. These columns are higher efficiency, and therefore provide higher-resolution separations.MAbPac SCX-10 columns are designed to address the regulatory requirements for biopharmaceutical characterization. The consistent manufacturing processes ensure reproducibility in methods development and data analysis.The MAbPac SCX-10 column is complimentary to the industry-leading Thermo Scientific™ ProPac™ WCX-10 column for MAb variant analysis. The MAbPac SCX-10 column offers alternative selectivity and provides higher resolution and efficiency for variant analysis of most MAb samples than the ProPac WCX-10 column. New MAbPac SCX Column Kits for Validation Now AvailableThree MAbPac SCX-10, 4 × 250 mm columns from one lot of resinThree MAbPac SCX-10, 4 × 250 mm columns, one each from three lots of resinThese MAbPac SCX-10 column kits will facilitate the validation process of MAbPac SCX-10 columns for MAb therapeutics development projects.The MAbPac SCX-10, 10 μm, 9 × 250 mm column is now available for semipreparative applications of MAb variant separations.Fast High-Resolution Analysis of MAb variants achieved in only 15 minutes!MAbPac SCX-10 Monoclonal Antibody Analysis Column SpecificationsColumn Dimensions10 μm: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm, 2 × 250 mm5 μm Analytical: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5 μm Analytical RS: 4.6 × 250 mm, 4.6 × 150 mm, 4.6 x 50mm, 2.1 x 250mm, 2.1 x 150mm, 2.1 x 250mm3 μm: 4 × 50 mmBead SizeUniform 10 μm, 5 μm, 3 μm particlesSubstrateHighly crosslinked DVB mediaPellicular LayerProprietary hydrophilicFunctional GroupSulfonicFlow Rate Range:0.2 to 2 mL/min for: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm0.1 to 0.5 ml/min for: 2 × 250 mmPressure Limit3,000 psi for 10 μm columns: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5,000 psi for Analytical 3 μm and 5 μm columns7,000 psi for Analytical RS 5 μm columnsTemperature Range:Ambient to 60 oCpH Range2–12Typical buffers:Sodium or potassium salts of phosphate, MESSolvents:50% acetonitrile if needed for cleaningShort Term Storage Solution (overnight):Your low salt concentration eluent (pH 3–10)Shipping Solution / Long Term Storage Solution:20 mM MES (pH 5.6) + 60 mM NaCl– + 0.1% sodium azideDetergent CompatibilityNonionic, anionic, or zwitterionic detergents. MAbPac SCX-10 RS ColumnsMAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 50 mm)082674MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 150 mm)085209MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 250 mm)082673MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 50mm)082675MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 150mm)088242MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 250mm)082515Analytical ColumnsMAbPac SCX-10, 3μm, Analytical Column (4 x 50 mm)077907MAbPac SCX-10, 5μm, Analytical Column (4 x 50 mm)078656MAbPac SCX-10, 5μm, Analytical Column (4 x 150 mm)085198MAbPac SCX-10, 5μm, Analytical Column (4 x 250 mm)078655MAbPac SCX-10, 10 μm, Analytical Column (4 x 250mm)074625MAbPac SCX-10, 10μm, Analytical Column (4 x 150mm)075602MAbPac SCX-10HT, 10μm, Analytical Column (4 x 50mm)075603MAbPac SCX-10, 10μm, Analytical Column (2 x 250mm)075604MAbPac SCX-10, 10μm, 9 x 250 MM columnSP6866MAbPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)SP6864MAPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)SP6865Guard ColumnsMAbPac SCX-10G, 10μm, Guard Column (4 x 50 mm)074631MAbPac SCX-10G, 10μm, Guard Column (2 x 50 mm)075749

MAbPac SCX-10 Monoclonal Antibody Analysis ColumnMAbPac SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of monoclonal antibody (MAb) variant samples.The MAbPac SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of MAbs and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of MAb variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.Features:? Unequalled high-resolution separations? Exceptionally high efficiency? Highest resolution of MAb variants? Unmatched column-to-column and lot-to-lot reproducibility? Elimination of hydrophobic interactions? Ideal for stability testingThe MAbPac resin technology is the basis for the superior performance of MAb variant analysis. The nonporous core particle provides high rates of mass transfer, which results in high-efficiency separations. A hydrophilic layer surrounds the polymeric beads, eliminating hydrophobic interactions between proteins and the resin while contributing to high-efficiency peaks. A proprietary grafted cation-exchange surface provides pH selectivity control, resulting in high-resolution separations. In addition, the MAbPac SCX-10 line of columns provides consistent column-to-column and lot-to-lot reproducibility for MAb variant separation results and is ideal for MAb therapeutic method development and high-throughput screening. The MAbPac SCX-10 RS columns are designed for use with the Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS system. These columns are packed in bioinert, PEEK-lined, stainless steel column hardware suitable for operation up to 10,000 psi. These columns are higher efficiency, and therefore provide higher-resolution separations.MAbPac SCX-10 columns are designed to address the regulatory requirements for biopharmaceutical characterization. The consistent manufacturing processes ensure reproducibility in methods development and data analysis.The MAbPac SCX-10 column is complimentary to the industry-leading Thermo Scientific™ ProPac™ WCX-10 column for MAb variant analysis. The MAbPac SCX-10 column offers alternative selectivity and provides higher resolution and efficiency for variant analysis of most MAb samples than the ProPac WCX-10 column. New MAbPac SCX Column Kits for Validation Now AvailableThree MAbPac SCX-10, 4 × 250 mm columns from one lot of resinThree MAbPac SCX-10, 4 × 250 mm columns, one each from three lots of resinThese MAbPac SCX-10 column kits will facilitate the validation process of MAbPac SCX-10 columns for MAb therapeutics development projects.The MAbPac SCX-10, 10 μm, 9 × 250 mm column is now available for semipreparative applications of MAb variant separations.Fast High-Resolution Analysis of MAb variants achieved in only 15 minutes!MAbPac SCX-10 Monoclonal Antibody Analysis Column SpecificationsColumn Dimensions10 μm: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm, 2 × 250 mm5 μm Analytical: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5 μm Analytical RS: 4.6 × 250 mm, 4.6 × 150 mm, 4.6 x 50mm, 2.1 x 250mm, 2.1 x 150mm, 2.1 x 250mm3 μm: 4 × 50 mmBead SizeUniform 10 μm, 5 μm, 3 μm particlesSubstrateHighly crosslinked DVB mediaPellicular LayerProprietary hydrophilicFunctional GroupSulfonicFlow Rate Range:0.2 to 2 mL/min for: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm0.1 to 0.5 ml/min for: 2 × 250 mmPressure Limit3,000 psi for 10 μm columns: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5,000 psi for Analytical 3 μm and 5 μm columns7,000 psi for Analytical RS 5 μm columnsTemperature Range:Ambient to 60 oCpH Range2–12Typical buffers:Sodium or potassium salts of phosphate, MESSolvents:50% acetonitrile if needed for cleaningShort Term Storage Solution (overnight):Your low salt concentration eluent (pH 3–10)Shipping Solution / Long Term Storage Solution:20 mM MES (pH 5.6) + 60 mM NaCl– + 0.1% sodium azideDetergent CompatibilityNonionic, anionic, or zwitterionic detergents. MAbPac SCX-10 RS ColumnsMAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 50 mm)082674MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 150 mm)085209MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 250 mm)082673MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 50mm)082675MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 150mm)088242MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 250mm)082515Analytical ColumnsMAbPac SCX-10, 3μm, Analytical Column (4 x 50 mm)077907MAbPac SCX-10, 5μm, Analytical Column (4 x 50 mm)078656MAbPac SCX-10, 5μm, Analytical Column (4 x 150 mm)085198MAbPac SCX-10, 5μm, Analytical Column (4 x 250 mm)078655MAbPac SCX-10, 10 μm, Analytical Column (4 x 250mm)074625MAbPac SCX-10, 10μm, Analytical Column (4 x 150mm)075602MAbPac SCX-10HT, 10μm, Analytical Column (4 x 50mm)075603MAbPac SCX-10, 10μm, Analytical Column (2 x 250mm)075604MAbPac SCX-10, 10μm, 9 x 250 MM columnSP6866MAbPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)SP6864MAPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)SP6865Guard ColumnsMAbPac SCX-10G, 10μm, Guard Column (4 x 50 mm)074631MAbPac SCX-10G, 10μm, Guard Column (2 x 50 mm)075749

MAbPac SCX-10 Monoclonal Antibody Analysis ColumnMAbPac SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of monoclonal antibody (MAb) variant samples.The MAbPac SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of MAbs and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of MAb variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.Features:? Unequalled high-resolution separations? Exceptionally high efficiency? Highest resolution of MAb variants? Unmatched column-to-column and lot-to-lot reproducibility? Elimination of hydrophobic interactions? Ideal for stability testingThe MAbPac resin technology is the basis for the superior performance of MAb variant analysis. The nonporous core particle provides high rates of mass transfer, which results in high-efficiency separations. A hydrophilic layer surrounds the polymeric beads, eliminating hydrophobic interactions between proteins and the resin while contributing to high-efficiency peaks. A proprietary grafted cation-exchange surface provides pH selectivity control, resulting in high-resolution separations. In addition, the MAbPac SCX-10 line of columns provides consistent column-to-column and lot-to-lot reproducibility for MAb variant separation results and is ideal for MAb therapeutic method development and high-throughput screening. The MAbPac SCX-10 RS columns are designed for use with the Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS system. These columns are packed in bioinert, PEEK-lined, stainless steel column hardware suitable for operation up to 10,000 psi. These columns are higher efficiency, and therefore provide higher-resolution separations.MAbPac SCX-10 columns are designed to address the regulatory requirements for biopharmaceutical characterization. The consistent manufacturing processes ensure reproducibility in methods development and data analysis.The MAbPac SCX-10 column is complimentary to the industry-leading Thermo Scientific™ ProPac™ WCX-10 column for MAb variant analysis. The MAbPac SCX-10 column offers alternative selectivity and provides higher resolution and efficiency for variant analysis of most MAb samples than the ProPac WCX-10 column. New MAbPac SCX Column Kits for Validation Now AvailableThree MAbPac SCX-10, 4 × 250 mm columns from one lot of resinThree MAbPac SCX-10, 4 × 250 mm columns, one each from three lots of resinThese MAbPac SCX-10 column kits will facilitate the validation process of MAbPac SCX-10 columns for MAb therapeutics development projects.The MAbPac SCX-10, 10 μm, 9 × 250 mm column is now available for semipreparative applications of MAb variant separations.Fast High-Resolution Analysis of MAb variants achieved in only 15 minutes!MAbPac SCX-10 Monoclonal Antibody Analysis Column SpecificationsColumn Dimensions10 μm: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm, 2 × 250 mm5 μm Analytical: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5 μm Analytical RS: 4.6 × 250 mm, 4.6 × 150 mm, 4.6 x 50mm, 2.1 x 250mm, 2.1 x 150mm, 2.1 x 250mm3 μm: 4 × 50 mmBead SizeUniform 10 μm, 5 μm, 3 μm particlesSubstrateHighly crosslinked DVB mediaPellicular LayerProprietary hydrophilicFunctional GroupSulfonicFlow Rate Range:0.2 to 2 mL/min for: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm0.1 to 0.5 ml/min for: 2 × 250 mmPressure Limit3,000 psi for 10 μm columns: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5,000 psi for Analytical 3 μm and 5 μm columns7,000 psi for Analytical RS 5 μm columnsTemperature Range:Ambient to 60 oCpH Range2–12Typical buffers:Sodium or potassium salts of phosphate, MESSolvents:50% acetonitrile if needed for cleaningShort Term Storage Solution (overnight):Your low salt concentration eluent (pH 3–10)Shipping Solution / Long Term Storage Solution:20 mM MES (pH 5.6) + 60 mM NaCl– + 0.1% sodium azideDetergent CompatibilityNonionic, anionic, or zwitterionic detergents. MAbPac SCX-10 RS ColumnsMAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 50 mm)082674MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 150 mm)085209MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 250 mm)082673MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 50mm)082675MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 150mm)088242MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 250mm)082515Analytical ColumnsMAbPac SCX-10, 3μm, Analytical Column (4 x 50 mm)077907MAbPac SCX-10, 5μm, Analytical Column (4 x 50 mm)078656MAbPac SCX-10, 5μm, Analytical Column (4 x 150 mm)085198MAbPac SCX-10, 5μm, Analytical Column (4 x 250 mm)078655MAbPac SCX-10, 10 μm, Analytical Column (4 x 250mm)074625MAbPac SCX-10, 10μm, Analytical Column (4 x 150mm)075602MAbPac SCX-10HT, 10μm, Analytical Column (4 x 50mm)075603MAbPac SCX-10, 10μm, Analytical Column (2 x 250mm)075604MAbPac SCX-10, 10μm, 9 x 250 MM columnSP6866MAbPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)SP6864MAPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)SP6865Guard ColumnsMAbPac SCX-10G, 10μm, Guard Column (4 x 50 mm)074631MAbPac SCX-10G, 10μm, Guard Column (2 x 50 mm)075749

MAbPac SCX-10 Monoclonal Antibody Analysis ColumnMAbPac SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of monoclonal antibody (MAb) variant samples.The MAbPac SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of MAbs and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of MAb variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.Features:? Unequalled high-resolution separations? Exceptionally high efficiency? Highest resolution of MAb variants? Unmatched column-to-column and lot-to-lot reproducibility? Elimination of hydrophobic interactions? Ideal for stability testingThe MAbPac resin technology is the basis for the superior performance of MAb variant analysis. The nonporous core particle provides high rates of mass transfer, which results in high-efficiency separations. A hydrophilic layer surrounds the polymeric beads, eliminating hydrophobic interactions between proteins and the resin while contributing to high-efficiency peaks. A proprietary grafted cation-exchange surface provides pH selectivity control, resulting in high-resolution separations. In addition, the MAbPac SCX-10 line of columns provides consistent column-to-column and lot-to-lot reproducibility for MAb variant separation results and is ideal for MAb therapeutic method development and high-throughput screening. The MAbPac SCX-10 RS columns are designed for use with the Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS system. These columns are packed in bioinert, PEEK-lined, stainless steel column hardware suitable for operation up to 10,000 psi. These columns are higher efficiency, and therefore provide higher-resolution separations.MAbPac SCX-10 columns are designed to address the regulatory requirements for biopharmaceutical characterization. The consistent manufacturing processes ensure reproducibility in methods development and data analysis.The MAbPac SCX-10 column is complimentary to the industry-leading Thermo Scientific™ ProPac™ WCX-10 column for MAb variant analysis. The MAbPac SCX-10 column offers alternative selectivity and provides higher resolution and efficiency for variant analysis of most MAb samples than the ProPac WCX-10 column. New MAbPac SCX Column Kits for Validation Now AvailableThree MAbPac SCX-10, 4 × 250 mm columns from one lot of resinThree MAbPac SCX-10, 4 × 250 mm columns, one each from three lots of resinThese MAbPac SCX-10 column kits will facilitate the validation process of MAbPac SCX-10 columns for MAb therapeutics development projects.The MAbPac SCX-10, 10 μm, 9 × 250 mm column is now available for semipreparative applications of MAb variant separations.Fast High-Resolution Analysis of MAb variants achieved in only 15 minutes!MAbPac SCX-10 Monoclonal Antibody Analysis Column SpecificationsColumn Dimensions10 μm: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm, 2 × 250 mm5 μm Analytical: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5 μm Analytical RS: 4.6 × 250 mm, 4.6 × 150 mm, 4.6 x 50mm, 2.1 x 250mm, 2.1 x 150mm, 2.1 x 250mm3 μm: 4 × 50 mmBead SizeUniform 10 μm, 5 μm, 3 μm particlesSubstrateHighly crosslinked DVB mediaPellicular LayerProprietary hydrophilicFunctional GroupSulfonicFlow Rate Range:0.2 to 2 mL/min for: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm0.1 to 0.5 ml/min for: 2 × 250 mmPressure Limit3,000 psi for 10 μm columns: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5,000 psi for Analytical 3 μm and 5 μm columns7,000 psi for Analytical RS 5 μm columnsTemperature Range:Ambient to 60 oCpH Range2–12Typical buffers:Sodium or potassium salts of phosphate, MESSolvents:50% acetonitrile if needed for cleaningShort Term Storage Solution (overnight):Your low salt concentration eluent (pH 3–10)Shipping Solution / Long Term Storage Solution:20 mM MES (pH 5.6) + 60 mM NaCl– + 0.1% sodium azideDetergent CompatibilityNonionic, anionic, or zwitterionic detergents. MAbPac SCX-10 RS ColumnsMAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 50 mm)082674MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 150 mm)085209MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 250 mm)082673MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 50mm)082675MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 150mm)088242MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 250mm)082515Analytical ColumnsMAbPac SCX-10, 3μm, Analytical Column (4 x 50 mm)077907MAbPac SCX-10, 5μm, Analytical Column (4 x 50 mm)078656MAbPac SCX-10, 5μm, Analytical Column (4 x 150 mm)085198MAbPac SCX-10, 5μm, Analytical Column (4 x 250 mm)078655MAbPac SCX-10, 10 μm, Analytical Column (4 x 250mm)074625MAbPac SCX-10, 10μm, Analytical Column (4 x 150mm)075602MAbPac SCX-10HT, 10μm, Analytical Column (4 x 50mm)075603MAbPac SCX-10, 10μm, Analytical Column (2 x 250mm)075604MAbPac SCX-10, 10μm, 9 x 250 MM columnSP6866MAbPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)SP6864MAPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)SP6865Guard ColumnsMAbPac SCX-10G, 10μm, Guard Column (4 x 50 mm)074631MAbPac SCX-10G, 10μm, Guard Column (2 x 50 mm)075749

MAbPac SEC-1 Monoclonal Antibody Analysis ColumnMAbPac SEC– 1 for Monoclonal Antibody (MAb) AnalysisMAbPac™ SEC-1 is a size exclusion chromatography (SEC) column designed for monoclonal antibody (MAb) analysis, including monomers, aggregates, and fragments, under non-denaturing conditions, and in both high- and low-salt mobile phases and volatile eluents.? Proprietary hydrophilic bonded layer results in minimal non-desired interactions between the biomolecules and the stationary phase.? Nonmetallic and Biocompatible PEEK™ housing eliminates metal contamination from the column hardware.? Stable surface bonding leads to low column bleed and compatibility with MS, ELSD and Corona® CAD detection.? Rugged, reproducible column.? Superior performance for the analysis of monoclonal antibodies, even at low salt concentrations. ApplicationsSEC is frequently used because of its simple operating principle and features not found in other separation techniques including separating aggregates that are not easily distinguished by other chromatographic means. MAbs produced from mammalian cell culture may contain significant amounts of dimers and higher-order aggregates. The MAbPac SEC-10 is a high-resolution SEC column that is suitable for use with a wide range of biomolecules, including monoclonal antibodies and their aggregates. The MAbPac SEC-1 column is based on high-purity, spherical, porous (300 ?), 5 μm silica particles covalently modified with a proprietary diol hydrophilic layer. The column is packed into nonmetallic, biocompatible, PEEK column housing to eliminate compromised chromatographic due to metal from the column hardware.Analytical ColumnsMAbPac SEC-1, 5μm 300 ?, analytical column (7.8 x 300mm)088460MAbPac SEC-1, 5μm, 300?, analytical column PEEK (4.0 x 300mm)074696MAbPac SEC-1, 5μm, 300?, analytical column PEEK (4.0 x 150mm)075592MAbPac SEC-1, 5μm 300 ?, analytical column (2.1 x 300mm)SP6937MAbPac SEC-1, 5μm 300 ?, analytical column(2.1 x 150mm)SP6938Guard ColumnsMAbPac SEC-1, 5μm, 300?, guard column PEEK (4.0 x 50mm)074697

MAbPac SEC-1 Monoclonal Antibody Analysis ColumnMAbPac SEC– 1 for Monoclonal Antibody (MAb) AnalysisMAbPac™ SEC-1 is a size exclusion chromatography (SEC) column designed for monoclonal antibody (MAb) analysis, including monomers, aggregates, and fragments, under non-denaturing conditions, and in both high- and low-salt mobile phases and volatile eluents.? Proprietary hydrophilic bonded layer results in minimal non-desired interactions between the biomolecules and the stationary phase.? Nonmetallic and Biocompatible PEEK™ housing eliminates metal contamination from the column hardware.? Stable surface bonding leads to low column bleed and compatibility with MS, ELSD and Corona® CAD detection.? Rugged, reproducible column.? Superior performance for the analysis of monoclonal antibodies, even at low salt concentrations. ApplicationsSEC is frequently used because of its simple operating principle and features not found in other separation techniques including separating aggregates that are not easily distinguished by other chromatographic means. MAbs produced from mammalian cell culture may contain significant amounts of dimers and higher-order aggregates. The MAbPac SEC-10 is a high-resolution SEC column that is suitable for use with a wide range of biomolecules, including monoclonal antibodies and their aggregates. The MAbPac SEC-1 column is based on high-purity, spherical, porous (300 ?), 5 μm silica particles covalently modified with a proprietary diol hydrophilic layer. The column is packed into nonmetallic, biocompatible, PEEK column housing to eliminate compromised chromatographic due to metal from the column hardware.Analytical ColumnsMAbPac SEC-1, 5μm 300 ?, analytical column (7.8 x 300mm)088460MAbPac SEC-1, 5μm, 300?, analytical column PEEK (4.0 x 300mm)074696MAbPac SEC-1, 5μm, 300?, analytical column PEEK (4.0 x 150mm)075592MAbPac SEC-1, 5μm 300 ?, analytical column (2.1 x 300mm)SP6937MAbPac SEC-1, 5μm 300 ?, analytical column(2.1 x 150mm)SP6938Guard ColumnsMAbPac SEC-1, 5μm, 300?, guard column PEEK (4.0 x 50mm)074697

MAbPac SCX-10 Monoclonal Antibody Analysis ColumnMAbPac SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of monoclonal antibody (MAb) variant samples.The MAbPac SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of MAbs and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of MAb variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.Features:? Unequalled high-resolution separations? Exceptionally high efficiency? Highest resolution of MAb variants? Unmatched column-to-column and lot-to-lot reproducibility? Elimination of hydrophobic interactions? Ideal for stability testingThe MAbPac resin technology is the basis for the superior performance of MAb variant analysis. The nonporous core particle provides high rates of mass transfer, which results in high-efficiency separations. A hydrophilic layer surrounds the polymeric beads, eliminating hydrophobic interactions between proteins and the resin while contributing to high-efficiency peaks. A proprietary grafted cation-exchange surface provides pH selectivity control, resulting in high-resolution separations. In addition, the MAbPac SCX-10 line of columns provides consistent column-to-column and lot-to-lot reproducibility for MAb variant separation results and is ideal for MAb therapeutic method development and high-throughput screening. The MAbPac SCX-10 RS columns are designed for use with the Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS system. These columns are packed in bioinert, PEEK-lined, stainless steel column hardware suitable for operation up to 10,000 psi. These columns are higher efficiency, and therefore provide higher-resolution separations.MAbPac SCX-10 columns are designed to address the regulatory requirements for biopharmaceutical characterization. The consistent manufacturing processes ensure reproducibility in methods development and data analysis.The MAbPac SCX-10 column is complimentary to the industry-leading Thermo Scientific™ ProPac™ WCX-10 column for MAb variant analysis. The MAbPac SCX-10 column offers alternative selectivity and provides higher resolution and efficiency for variant analysis of most MAb samples than the ProPac WCX-10 column. New MAbPac SCX Column Kits for Validation Now AvailableThree MAbPac SCX-10, 4 × 250 mm columns from one lot of resinThree MAbPac SCX-10, 4 × 250 mm columns, one each from three lots of resinThese MAbPac SCX-10 column kits will facilitate the validation process of MAbPac SCX-10 columns for MAb therapeutics development projects.The MAbPac SCX-10, 10 μm, 9 × 250 mm column is now available for semipreparative applications of MAb variant separations.Fast High-Resolution Analysis of MAb variants achieved in only 15 minutes!MAbPac SCX-10 Monoclonal Antibody Analysis Column SpecificationsColumn Dimensions10 μm: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm, 2 × 250 mm5 μm Analytical: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5 μm Analytical RS: 4.6 × 250 mm, 4.6 × 150 mm, 4.6 x 50mm, 2.1 x 250mm, 2.1 x 150mm, 2.1 x 250mm3 μm: 4 × 50 mmBead SizeUniform 10 μm, 5 μm, 3 μm particlesSubstrateHighly crosslinked DVB mediaPellicular LayerProprietary hydrophilicFunctional GroupSulfonicFlow Rate Range:0.2 to 2 mL/min for: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm0.1 to 0.5 ml/min for: 2 × 250 mmPressure Limit3,000 psi for 10 μm columns: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5,000 psi for Analytical 3 μm and 5 μm columns7,000 psi for Analytical RS 5 μm columnsTemperature Range:Ambient to 60 oCpH Range2–12Typical buffers:Sodium or potassium salts of phosphate, MESSolvents:50% acetonitrile if needed for cleaningShort Term Storage Solution (overnight):Your low salt concentration eluent (pH 3–10)Shipping Solution / Long Term Storage Solution:20 mM MES (pH 5.6) + 60 mM NaCl– + 0.1% sodium azideDetergent CompatibilityNonionic, anionic, or zwitterionic detergents. MAbPac SCX-10 RS ColumnsMAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 50 mm)082674MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 150 mm)085209MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 250 mm)082673MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 50mm)082675MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 150mm)088242MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 250mm)082515Analytical ColumnsMAbPac SCX-10, 3μm, Analytical Column (4 x 50 mm)077907MAbPac SCX-10, 5μm, Analytical Column (4 x 50 mm)078656MAbPac SCX-10, 5μm, Analytical Column (4 x 150 mm)085198MAbPac SCX-10, 5μm, Analytical Column (4 x 250 mm)078655MAbPac SCX-10, 10 μm, Analytical Column (4 x 250mm)074625MAbPac SCX-10, 10μm, Analytical Column (4 x 150mm)075602MAbPac SCX-10HT, 10μm, Analytical Column (4 x 50mm)075603MAbPac SCX-10, 10μm, Analytical Column (2 x 250mm)075604MAbPac SCX-10, 10μm, 9 x 250 MM columnSP6866MAbPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)SP6864MAPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)SP6865Guard ColumnsMAbPac SCX-10G, 10μm, Guard Column (4 x 50 mm)074631MAbPac SCX-10G, 10μm, Guard Column (2 x 50 mm)075749

MAbPac SCX-10 Monoclonal Antibody Analysis ColumnMAbPac SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of monoclonal antibody (MAb) variant samples.The MAbPac SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of MAbs and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of MAb variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.Features:? Unequalled high-resolution separations? Exceptionally high efficiency? Highest resolution of MAb variants? Unmatched column-to-column and lot-to-lot reproducibility? Elimination of hydrophobic interactions? Ideal for stability testingThe MAbPac resin technology is the basis for the superior performance of MAb variant analysis. The nonporous core particle provides high rates of mass transfer, which results in high-efficiency separations. A hydrophilic layer surrounds the polymeric beads, eliminating hydrophobic interactions between proteins and the resin while contributing to high-efficiency peaks. A proprietary grafted cation-exchange surface provides pH selectivity control, resulting in high-resolution separations. In addition, the MAbPac SCX-10 line of columns provides consistent column-to-column and lot-to-lot reproducibility for MAb variant separation results and is ideal for MAb therapeutic method development and high-throughput screening. The MAbPac SCX-10 RS columns are designed for use with the Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS system. These columns are packed in bioinert, PEEK-lined, stainless steel column hardware suitable for operation up to 10,000 psi. These columns are higher efficiency, and therefore provide higher-resolution separations.MAbPac SCX-10 columns are designed to address the regulatory requirements for biopharmaceutical characterization. The consistent manufacturing processes ensure reproducibility in methods development and data analysis.The MAbPac SCX-10 column is complimentary to the industry-leading Thermo Scientific™ ProPac™ WCX-10 column for MAb variant analysis. The MAbPac SCX-10 column offers alternative selectivity and provides higher resolution and efficiency for variant analysis of most MAb samples than the ProPac WCX-10 column. New MAbPac SCX Column Kits for Validation Now AvailableThree MAbPac SCX-10, 4 × 250 mm columns from one lot of resinThree MAbPac SCX-10, 4 × 250 mm columns, one each from three lots of resinThese MAbPac SCX-10 column kits will facilitate the validation process of MAbPac SCX-10 columns for MAb therapeutics development projects.The MAbPac SCX-10, 10 μm, 9 × 250 mm column is now available for semipreparative applications of MAb variant separations.Fast High-Resolution Analysis of MAb variants achieved in only 15 minutes!MAbPac SCX-10 Monoclonal Antibody Analysis Column SpecificationsColumn Dimensions10 μm: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm, 2 × 250 mm5 μm Analytical: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5 μm Analytical RS: 4.6 × 250 mm, 4.6 × 150 mm, 4.6 x 50mm, 2.1 x 250mm, 2.1 x 150mm, 2.1 x 250mm3 μm: 4 × 50 mmBead SizeUniform 10 μm, 5 μm, 3 μm particlesSubstrateHighly crosslinked DVB mediaPellicular LayerProprietary hydrophilicFunctional GroupSulfonicFlow Rate Range:0.2 to 2 mL/min for: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm0.1 to 0.5 ml/min for: 2 × 250 mmPressure Limit3,000 psi for 10 μm columns: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5,000 psi for Analytical 3 μm and 5 μm columns7,000 psi for Analytical RS 5 μm columnsTemperature Range:Ambient to 60 oCpH Range2–12Typical buffers:Sodium or potassium salts of phosphate, MESSolvents:50% acetonitrile if needed for cleaningShort Term Storage Solution (overnight):Your low salt concentration eluent (pH 3–10)Shipping Solution / Long Term Storage Solution:20 mM MES (pH 5.6) + 60 mM NaCl– + 0.1% sodium azideDetergent CompatibilityNonionic, anionic, or zwitterionic detergents. MAbPac SCX-10 RS ColumnsMAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 50 mm)082674MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 150 mm)085209MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 250 mm)082673MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 50mm)082675MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 150mm)088242MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 250mm)082515Analytical ColumnsMAbPac SCX-10, 3μm, Analytical Column (4 x 50 mm)077907MAbPac SCX-10, 5μm, Analytical Column (4 x 50 mm)078656MAbPac SCX-10, 5μm, Analytical Column (4 x 150 mm)085198MAbPac SCX-10, 5μm, Analytical Column (4 x 250 mm)078655MAbPac SCX-10, 10 μm, Analytical Column (4 x 250mm)074625MAbPac SCX-10, 10μm, Analytical Column (4 x 150mm)075602MAbPac SCX-10HT, 10μm, Analytical Column (4 x 50mm)075603MAbPac SCX-10, 10μm, Analytical Column (2 x 250mm)075604MAbPac SCX-10, 10μm, 9 x 250 MM columnSP6866MAbPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)SP6864MAPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)SP6865Guard ColumnsMAbPac SCX-10G, 10μm, Guard Column (4 x 50 mm)074631MAbPac SCX-10G, 10μm, Guard Column (2 x 50 mm)075749

MAbPac SCX-10 Monoclonal Antibody Analysis ColumnMAbPac SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of monoclonal antibody (MAb) variant samples.The MAbPac SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of MAbs and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of MAb variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.Features:? Unequalled high-resolution separations? Exceptionally high efficiency? Highest resolution of MAb variants? Unmatched column-to-column and lot-to-lot reproducibility? Elimination of hydrophobic interactions? Ideal for stability testingThe MAbPac resin technology is the basis for the superior performance of MAb variant analysis. The nonporous core particle provides high rates of mass transfer, which results in high-efficiency separations. A hydrophilic layer surrounds the polymeric beads, eliminating hydrophobic interactions between proteins and the resin while contributing to high-efficiency peaks. A proprietary grafted cation-exchange surface provides pH selectivity control, resulting in high-resolution separations. In addition, the MAbPac SCX-10 line of columns provides consistent column-to-column and lot-to-lot reproducibility for MAb variant separation results and is ideal for MAb therapeutic method development and high-throughput screening. The MAbPac SCX-10 RS columns are designed for use with the Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS system. These columns are packed in bioinert, PEEK-lined, stainless steel column hardware suitable for operation up to 10,000 psi. These columns are higher efficiency, and therefore provide higher-resolution separations.MAbPac SCX-10 columns are designed to address the regulatory requirements for biopharmaceutical characterization. The consistent manufacturing processes ensure reproducibility in methods development and data analysis.The MAbPac SCX-10 column is complimentary to the industry-leading Thermo Scientific™ ProPac™ WCX-10 column for MAb variant analysis. The MAbPac SCX-10 column offers alternative selectivity and provides higher resolution and efficiency for variant analysis of most MAb samples than the ProPac WCX-10 column. New MAbPac SCX Column Kits for Validation Now AvailableThree MAbPac SCX-10, 4 × 250 mm columns from one lot of resinThree MAbPac SCX-10, 4 × 250 mm columns, one each from three lots of resinThese MAbPac SCX-10 column kits will facilitate the validation process of MAbPac SCX-10 columns for MAb therapeutics development projects.The MAbPac SCX-10, 10 μm, 9 × 250 mm column is now available for semipreparative applications of MAb variant separations.Fast High-Resolution Analysis of MAb variants achieved in only 15 minutes!MAbPac SCX-10 Monoclonal Antibody Analysis Column SpecificationsColumn Dimensions10 μm: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm, 2 × 250 mm5 μm Analytical: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5 μm Analytical RS: 4.6 × 250 mm, 4.6 × 150 mm, 4.6 x 50mm, 2.1 x 250mm, 2.1 x 150mm, 2.1 x 250mm3 μm: 4 × 50 mmBead SizeUniform 10 μm, 5 μm, 3 μm particlesSubstrateHighly crosslinked DVB mediaPellicular LayerProprietary hydrophilicFunctional GroupSulfonicFlow Rate Range:0.2 to 2 mL/min for: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm0.1 to 0.5 ml/min for: 2 × 250 mmPressure Limit3,000 psi for 10 μm columns: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5,000 psi for Analytical 3 μm and 5 μm columns7,000 psi for Analytical RS 5 μm columnsTemperature Range:Ambient to 60 oCpH Range2–12Typical buffers:Sodium or potassium salts of phosphate, MESSolvents:50% acetonitrile if needed for cleaningShort Term Storage Solution (overnight):Your low salt concentration eluent (pH 3–10)Shipping Solution / Long Term Storage Solution:20 mM MES (pH 5.6) + 60 mM NaCl– + 0.1% sodium azideDetergent CompatibilityNonionic, anionic, or zwitterionic detergents. MAbPac SCX-10 RS ColumnsMAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 50 mm)082674MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 150 mm)085209MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 250 mm)082673MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 50mm)082675MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 150mm)088242MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 250mm)082515Analytical ColumnsMAbPac SCX-10, 3μm, Analytical Column (4 x 50 mm)077907MAbPac SCX-10, 5μm, Analytical Column (4 x 50 mm)078656MAbPac SCX-10, 5μm, Analytical Column (4 x 150 mm)085198MAbPac SCX-10, 5μm, Analytical Column (4 x 250 mm)078655MAbPac SCX-10, 10 μm, Analytical Column (4 x 250mm)074625MAbPac SCX-10, 10μm, Analytical Column (4 x 150mm)075602MAbPac SCX-10HT, 10μm, Analytical Column (4 x 50mm)075603MAbPac SCX-10, 10μm, Analytical Column (2 x 250mm)075604MAbPac SCX-10, 10μm, 9 x 250 MM columnSP6866MAbPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)SP6864MAPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)SP6865Guard ColumnsMAbPac SCX-10G, 10μm, Guard Column (4 x 50 mm)074631MAbPac SCX-10G, 10μm, Guard Column (2 x 50 mm)075749

MAbPac SCX-10 Monoclonal Antibody Analysis ColumnMAbPac SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of monoclonal antibody (MAb) variant samples.The MAbPac SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of MAbs and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of MAb variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.Features:? Unequalled high-resolution separations? Exceptionally high efficiency? Highest resolution of MAb variants? Unmatched column-to-column and lot-to-lot reproducibility? Elimination of hydrophobic interactions? Ideal for stability testingThe MAbPac resin technology is the basis for the superior performance of MAb variant analysis. The nonporous core particle provides high rates of mass transfer, which results in high-efficiency separations. A hydrophilic layer surrounds the polymeric beads, eliminating hydrophobic interactions between proteins and the resin while contributing to high-efficiency peaks. A proprietary grafted cation-exchange surface provides pH selectivity control, resulting in high-resolution separations. In addition, the MAbPac SCX-10 line of columns provides consistent column-to-column and lot-to-lot reproducibility for MAb variant separation results and is ideal for MAb therapeutic method development and high-throughput screening. The MAbPac SCX-10 RS columns are designed for use with the Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS system. These columns are packed in bioinert, PEEK-lined, stainless steel column hardware suitable for operation up to 10,000 psi. These columns are higher efficiency, and therefore provide higher-resolution separations.MAbPac SCX-10 columns are designed to address the regulatory requirements for biopharmaceutical characterization. The consistent manufacturing processes ensure reproducibility in methods development and data analysis.The MAbPac SCX-10 column is complimentary to the industry-leading Thermo Scientific™ ProPac™ WCX-10 column for MAb variant analysis. The MAbPac SCX-10 column offers alternative selectivity and provides higher resolution and efficiency for variant analysis of most MAb samples than the ProPac WCX-10 column. New MAbPac SCX Column Kits for Validation Now AvailableThree MAbPac SCX-10, 4 × 250 mm columns from one lot of resinThree MAbPac SCX-10, 4 × 250 mm columns, one each from three lots of resinThese MAbPac SCX-10 column kits will facilitate the validation process of MAbPac SCX-10 columns for MAb therapeutics development projects.The MAbPac SCX-10, 10 μm, 9 × 250 mm column is now available for semipreparative applications of MAb variant separations.Fast High-Resolution Analysis of MAb variants achieved in only 15 minutes!MAbPac SCX-10 Monoclonal Antibody Analysis Column SpecificationsColumn Dimensions10 μm: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm, 2 × 250 mm5 μm Analytical: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5 μm Analytical RS: 4.6 × 250 mm, 4.6 × 150 mm, 4.6 x 50mm, 2.1 x 250mm, 2.1 x 150mm, 2.1 x 250mm3 μm: 4 × 50 mmBead SizeUniform 10 μm, 5 μm, 3 μm particlesSubstrateHighly crosslinked DVB mediaPellicular LayerProprietary hydrophilicFunctional GroupSulfonicFlow Rate Range:0.2 to 2 mL/min for: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm0.1 to 0.5 ml/min for: 2 × 250 mmPressure Limit3,000 psi for 10 μm columns: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5,000 psi for Analytical 3 μm and 5 μm columns7,000 psi for Analytical RS 5 μm columnsTemperature Range:Ambient to 60 oCpH Range2–12Typical buffers:Sodium or potassium salts of phosphate, MESSolvents:50% acetonitrile if needed for cleaningShort Term Storage Solution (overnight):Your low salt concentration eluent (pH 3–10)Shipping Solution / Long Term Storage Solution:20 mM MES (pH 5.6) + 60 mM NaCl– + 0.1% sodium azideDetergent CompatibilityNonionic, anionic, or zwitterionic detergents. MAbPac SCX-10 RS ColumnsMAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 50 mm)082674MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 150 mm)085209MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 250 mm)082673MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 50mm)082675MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 150mm)088242MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 250mm)082515Analytical ColumnsMAbPac SCX-10, 3μm, Analytical Column (4 x 50 mm)077907MAbPac SCX-10, 5μm, Analytical Column (4 x 50 mm)078656MAbPac SCX-10, 5μm, Analytical Column (4 x 150 mm)085198MAbPac SCX-10, 5μm, Analytical Column (4 x 250 mm)078655MAbPac SCX-10, 10 μm, Analytical Column (4 x 250mm)074625MAbPac SCX-10, 10μm, Analytical Column (4 x 150mm)075602MAbPac SCX-10HT, 10μm, Analytical Column (4 x 50mm)075603MAbPac SCX-10, 10μm, Analytical Column (2 x 250mm)075604MAbPac SCX-10, 10μm, 9 x 250 MM columnSP6866MAbPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)SP6864MAPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)SP6865Guard ColumnsMAbPac SCX-10G, 10μm, Guard Column (4 x 50 mm)074631MAbPac SCX-10G, 10μm, Guard Column (2 x 50 mm)075749

MAbPac SCX-10 Monoclonal Antibody Analysis ColumnMAbPac SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of monoclonal antibody (MAb) variant samples.The MAbPac SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of MAbs and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of MAb variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.Features:? Unequalled high-resolution separations? Exceptionally high efficiency? Highest resolution of MAb variants? Unmatched column-to-column and lot-to-lot reproducibility? Elimination of hydrophobic interactions? Ideal for stability testingThe MAbPac resin technology is the basis for the superior performance of MAb variant analysis. The nonporous core particle provides high rates of mass transfer, which results in high-efficiency separations. A hydrophilic layer surrounds the polymeric beads, eliminating hydrophobic interactions between proteins and the resin while contributing to high-efficiency peaks. A proprietary grafted cation-exchange surface provides pH selectivity control, resulting in high-resolution separations. In addition, the MAbPac SCX-10 line of columns provides consistent column-to-column and lot-to-lot reproducibility for MAb variant separation results and is ideal for MAb therapeutic method development and high-throughput screening. The MAbPac SCX-10 RS columns are designed for use with the Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS system. These columns are packed in bioinert, PEEK-lined, stainless steel column hardware suitable for operation up to 10,000 psi. These columns are higher efficiency, and therefore provide higher-resolution separations.MAbPac SCX-10 columns are designed to address the regulatory requirements for biopharmaceutical characterization. The consistent manufacturing processes ensure reproducibility in methods development and data analysis.The MAbPac SCX-10 column is complimentary to the industry-leading Thermo Scientific™ ProPac™ WCX-10 column for MAb variant analysis. The MAbPac SCX-10 column offers alternative selectivity and provides higher resolution and efficiency for variant analysis of most MAb samples than the ProPac WCX-10 column. New MAbPac SCX Column Kits for Validation Now AvailableThree MAbPac SCX-10, 4 × 250 mm columns from one lot of resinThree MAbPac SCX-10, 4 × 250 mm columns, one each from three lots of resinThese MAbPac SCX-10 column kits will facilitate the validation process of MAbPac SCX-10 columns for MAb therapeutics development projects.The MAbPac SCX-10, 10 μm, 9 × 250 mm column is now available for semipreparative applications of MAb variant separations.Fast High-Resolution Analysis of MAb variants achieved in only 15 minutes!MAbPac SCX-10 Monoclonal Antibody Analysis Column SpecificationsColumn Dimensions10 μm: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm, 2 × 250 mm5 μm Analytical: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5 μm Analytical RS: 4.6 × 250 mm, 4.6 × 150 mm, 4.6 x 50mm, 2.1 x 250mm, 2.1 x 150mm, 2.1 x 250mm3 μm: 4 × 50 mmBead SizeUniform 10 μm, 5 μm, 3 μm particlesSubstrateHighly crosslinked DVB mediaPellicular LayerProprietary hydrophilicFunctional GroupSulfonicFlow Rate Range:0.2 to 2 mL/min for: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm0.1 to 0.5 ml/min for: 2 × 250 mmPressure Limit3,000 psi for 10 μm columns: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5,000 psi for Analytical 3 μm and 5 μm columns7,000 psi for Analytical RS 5 μm columnsTemperature Range:Ambient to 60 oCpH Range2–12Typical buffers:Sodium or potassium salts of phosphate, MESSolvents:50% acetonitrile if needed for cleaningShort Term Storage Solution (overnight):Your low salt concentration eluent (pH 3–10)Shipping Solution / Long Term Storage Solution:20 mM MES (pH 5.6) + 60 mM NaCl– + 0.1% sodium azideDetergent CompatibilityNonionic, anionic, or zwitterionic detergents. MAbPac SCX-10 RS ColumnsMAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 50 mm)082674MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 150 mm)085209MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 250 mm)082673MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 50mm)082675MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 150mm)088242MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 250mm)082515Analytical ColumnsMAbPac SCX-10, 3μm, Analytical Column (4 x 50 mm)077907MAbPac SCX-10, 5μm, Analytical Column (4 x 50 mm)078656MAbPac SCX-10, 5μm, Analytical Column (4 x 150 mm)085198MAbPac SCX-10, 5μm, Analytical Column (4 x 250 mm)078655MAbPac SCX-10, 10 μm, Analytical Column (4 x 250mm)074625MAbPac SCX-10, 10μm, Analytical Column (4 x 150mm)075602MAbPac SCX-10HT, 10μm, Analytical Column (4 x 50mm)075603MAbPac SCX-10, 10μm, Analytical Column (2 x 250mm)075604MAbPac SCX-10, 10μm, 9 x 250 MM columnSP6866MAbPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)SP6864MAPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)SP6865Guard ColumnsMAbPac SCX-10G, 10μm, Guard Column (4 x 50 mm)074631MAbPac SCX-10G, 10μm, Guard Column (2 x 50 mm)075749

MAbPac SCX-10 Monoclonal Antibody Analysis ColumnMAbPac SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of monoclonal antibody (MAb) variant samples.The MAbPac SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of MAbs and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of MAb variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.Features:? Unequalled high-resolution separations? Exceptionally high efficiency? Highest resolution of MAb variants? Unmatched column-to-column and lot-to-lot reproducibility? Elimination of hydrophobic interactions? Ideal for stability testingThe MAbPac resin technology is the basis for the superior performance of MAb variant analysis. The nonporous core particle provides high rates of mass transfer, which results in high-efficiency separations. A hydrophilic layer surrounds the polymeric beads, eliminating hydrophobic interactions between proteins and the resin while contributing to high-efficiency peaks. A proprietary grafted cation-exchange surface provides pH selectivity control, resulting in high-resolution separations. In addition, the MAbPac SCX-10 line of columns provides consistent column-to-column and lot-to-lot reproducibility for MAb variant separation results and is ideal for MAb therapeutic method development and high-throughput screening. The MAbPac SCX-10 RS columns are designed for use with the Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS system. These columns are packed in bioinert, PEEK-lined, stainless steel column hardware suitable for operation up to 10,000 psi. These columns are higher efficiency, and therefore provide higher-resolution separations.MAbPac SCX-10 columns are designed to address the regulatory requirements for biopharmaceutical characterization. The consistent manufacturing processes ensure reproducibility in methods development and data analysis.The MAbPac SCX-10 column is complimentary to the industry-leading Thermo Scientific™ ProPac™ WCX-10 column for MAb variant analysis. The MAbPac SCX-10 column offers alternative selectivity and provides higher resolution and efficiency for variant analysis of most MAb samples than the ProPac WCX-10 column. New MAbPac SCX Column Kits for Validation Now AvailableThree MAbPac SCX-10, 4 × 250 mm columns from one lot of resinThree MAbPac SCX-10, 4 × 250 mm columns, one each from three lots of resinThese MAbPac SCX-10 column kits will facilitate the validation process of MAbPac SCX-10 columns for MAb therapeutics development projects.The MAbPac SCX-10, 10 μm, 9 × 250 mm column is now available for semipreparative applications of MAb variant separations.Fast High-Resolution Analysis of MAb variants achieved in only 15 minutes!MAbPac SCX-10 Monoclonal Antibody Analysis Column SpecificationsColumn Dimensions10 μm: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm, 2 × 250 mm5 μm Analytical: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5 μm Analytical RS: 4.6 × 250 mm, 4.6 × 150 mm, 4.6 x 50mm, 2.1 x 250mm, 2.1 x 150mm, 2.1 x 250mm3 μm: 4 × 50 mmBead SizeUniform 10 μm, 5 μm, 3 μm particlesSubstrateHighly crosslinked DVB mediaPellicular LayerProprietary hydrophilicFunctional GroupSulfonicFlow Rate Range:0.2 to 2 mL/min for: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm0.1 to 0.5 ml/min for: 2 × 250 mmPressure Limit3,000 psi for 10 μm columns: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5,000 psi for Analytical 3 μm and 5 μm columns7,000 psi for Analytical RS 5 μm columnsTemperature Range:Ambient to 60 oCpH Range2–12Typical buffers:Sodium or potassium salts of phosphate, MESSolvents:50% acetonitrile if needed for cleaningShort Term Storage Solution (overnight):Your low salt concentration eluent (pH 3–10)Shipping Solution / Long Term Storage Solution:20 mM MES (pH 5.6) + 60 mM NaCl– + 0.1% sodium azideDetergent CompatibilityNonionic, anionic, or zwitterionic detergents. MAbPac SCX-10 RS ColumnsMAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 50 mm)082674MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 150 mm)085209MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 250 mm)082673MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 50mm)082675MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 150mm)088242MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 250mm)082515Analytical ColumnsMAbPac SCX-10, 3μm, Analytical Column (4 x 50 mm)077907MAbPac SCX-10, 5μm, Analytical Column (4 x 50 mm)078656MAbPac SCX-10, 5μm, Analytical Column (4 x 150 mm)085198MAbPac SCX-10, 5μm, Analytical Column (4 x 250 mm)078655MAbPac SCX-10, 10 μm, Analytical Column (4 x 250mm)074625MAbPac SCX-10, 10μm, Analytical Column (4 x 150mm)075602MAbPac SCX-10HT, 10μm, Analytical Column (4 x 50mm)075603MAbPac SCX-10, 10μm, Analytical Column (2 x 250mm)075604MAbPac SCX-10, 10μm, 9 x 250 MM columnSP6866MAbPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)SP6864MAPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)SP6865Guard ColumnsMAbPac SCX-10G, 10μm, Guard Column (4 x 50 mm)074631MAbPac SCX-10G, 10μm, Guard Column (2 x 50 mm)075749

MAbPac SCX-10 Monoclonal Antibody Analysis ColumnMAbPac SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of monoclonal antibody (MAb) variant samples.The MAbPac SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of MAbs and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of MAb variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.Features:? Unequalled high-resolution separations? Exceptionally high efficiency? Highest resolution of MAb variants? Unmatched column-to-column and lot-to-lot reproducibility? Elimination of hydrophobic interactions? Ideal for stability testingThe MAbPac resin technology is the basis for the superior performance of MAb variant analysis. The nonporous core particle provides high rates of mass transfer, which results in high-efficiency separations. A hydrophilic layer surrounds the polymeric beads, eliminating hydrophobic interactions between proteins and the resin while contributing to high-efficiency peaks. A proprietary grafted cation-exchange surface provides pH selectivity control, resulting in high-resolution separations. In addition, the MAbPac SCX-10 line of columns provides consistent column-to-column and lot-to-lot reproducibility for MAb variant separation results and is ideal for MAb therapeutic method development and high-throughput screening. The MAbPac SCX-10 RS columns are designed for use with the Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS system. These columns are packed in bioinert, PEEK-lined, stainless steel column hardware suitable for operation up to 10,000 psi. These columns are higher efficiency, and therefore provide higher-resolution separations.MAbPac SCX-10 columns are designed to address the regulatory requirements for biopharmaceutical characterization. The consistent manufacturing processes ensure reproducibility in methods development and data analysis.The MAbPac SCX-10 column is complimentary to the industry-leading Thermo Scientific™ ProPac™ WCX-10 column for MAb variant analysis. The MAbPac SCX-10 column offers alternative selectivity and provides higher resolution and efficiency for variant analysis of most MAb samples than the ProPac WCX-10 column. New MAbPac SCX Column Kits for Validation Now AvailableThree MAbPac SCX-10, 4 × 250 mm columns from one lot of resinThree MAbPac SCX-10, 4 × 250 mm columns, one each from three lots of resinThese MAbPac SCX-10 column kits will facilitate the validation process of MAbPac SCX-10 columns for MAb therapeutics development projects.The MAbPac SCX-10, 10 μm, 9 × 250 mm column is now available for semipreparative applications of MAb variant separations.Fast High-Resolution Analysis of MAb variants achieved in only 15 minutes!MAbPac SCX-10 Monoclonal Antibody Analysis Column SpecificationsColumn Dimensions10 μm: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm, 2 × 250 mm5 μm Analytical: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5 μm Analytical RS: 4.6 × 250 mm, 4.6 × 150 mm, 4.6 x 50mm, 2.1 x 250mm, 2.1 x 150mm, 2.1 x 250mm3 μm: 4 × 50 mmBead SizeUniform 10 μm, 5 μm, 3 μm particlesSubstrateHighly crosslinked DVB mediaPellicular LayerProprietary hydrophilicFunctional GroupSulfonicFlow Rate Range:0.2 to 2 mL/min for: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm0.1 to 0.5 ml/min for: 2 × 250 mmPressure Limit3,000 psi for 10 μm columns: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm5,000 psi for Analytical 3 μm and 5 μm columns7,000 psi for Analytical RS 5 μm columnsTemperature Range:Ambient to 60 oCpH Range2–12Typical buffers:Sodium or potassium salts of phosphate, MESSolvents:50% acetonitrile if needed for cleaningShort Term Storage Solution (overnight):Your low salt concentration eluent (pH 3–10)Shipping Solution / Long Term Storage Solution:20 mM MES (pH 5.6) + 60 mM NaCl– + 0.1% sodium azideDetergent CompatibilityNonionic, anionic, or zwitterionic detergents. MAbPac SCX-10 RS ColumnsMAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 50 mm)082674MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 150 mm)085209MAbPac SCX-10 RS, 5μm, Analytical Column (4.6 x 250 mm)082673MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 50mm)082675MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 150mm)088242MAbPac SCX-10 RS, 5μm, Analytical Column (2.1 x 250mm)082515Analytical ColumnsMAbPac SCX-10, 3μm, Analytical Column (4 x 50 mm)077907MAbPac SCX-10, 5μm, Analytical Column (4 x 50 mm)078656MAbPac SCX-10, 5μm, Analytical Column (4 x 150 mm)085198MAbPac SCX-10, 5μm, Analytical Column (4 x 250 mm)078655MAbPac SCX-10, 10 μm, Analytical Column (4 x 250mm)074625MAbPac SCX-10, 10μm, Analytical Column (4 x 150mm)075602MAbPac SCX-10HT, 10μm, Analytical Column (4 x 50mm)075603MAbPac SCX-10, 10μm, Analytical Column (2 x 250mm)075604MAbPac SCX-10, 10μm, 9 x 250 MM columnSP6866MAbPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)SP6864MAPac SCX-10, 10μm, Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)SP6865Guard ColumnsMAbPac SCX-10G, 10μm, Guard Column (4 x 50 mm)074631MAbPac SCX-10G, 10μm, Guard Column (2 x 50 mm)075749