SPECORD 250用于血液的光度分析

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analytikjenaAnalytik Jena AG Photometric analysis of blood in SPECORD250 As a result of its light scattering properties， the photometric analysis of blood represents aspecial application. The light scattered by the turbidity of the sample falsified themeasurement results. For such measurements， there are special positions in SPECORDQfor turbid samples located in the sample volume directly in front of the radiation receiver. The blood samples were measured using 2 different cuvettes with separable window plates，with 10 pm and 100 pm coating thicknesses. The cuvette units consist of two separablequartz window plates. These are anchored in a holder used for this purpose. The outerdimensions of a cuvette are obtained and no special cuvette holder is required. The followingparameter settings were made： Cycle：noneMeasurement mode：：1registrationCorrection：referenceRange [nm]： 190-1100Display：absorptionStep width [nm]： 1Lamp changeover：320 nmIntegration time [s] 0.02Slit：2 nmAccessories none Absorption It may be observed that the quartz cuvette with 100 pm coating thickness is better suited forthis measurement. The absorption of haemoglobin in blood at 542 nm and 577 nm is very low with a 10 pm coating thickness. For this reason， further work was the carried out with a100 pm coating thickness. The measurements with a 100 pm coating thickness were then carried out with the sameparameter settings in the turbid sample position. Absorption The absorption of the sample background in the turbid sample position compared with thenormal position was significantly reduced by eliminating the stray light (e.g. absorption above600 nm in the normal position approx. 0.7 A and in the turbid position approx. 0.1 A). Theholder openings in front of the radiation receiver for strongly scattering samples are thereforevery well suited for the measurement of blood. Expression and further use with indication of source permit. C2005 Analytik Jena AG Publisher：Analytik Jena AGKonrad-Zuse-StraBe 107745 Jena Phone +49 (0) 36 41/77-70Fax +49 (0)3641/77-92 79 www.analytik-jena.cominfo@analytik-jena.com Germany Photometric analysis of blood/Reference No.： UV ALL e|Oz