发酵罐在毕节酵母菌培养中的应用

智能文字提取功能测试中

发酵罐在毕节酵母菌培养中的应用酵母菌是一种单细胞的真核微生物。酵母菌是个通俗名称，是以芽殖或裂殖来进行无性繁殖的单细胞真菌的统称，与以霉菌区分开。极少数种可以产生子囊孢子进行有性繁殖。酵母菌主要分布在含糖质较高的偏酸性环境，如各种水果的表皮、发酵的果汁、蔬菜、花蜜、植物叶面、菜园果园土壤和酒曲中。他们多为腐生菌，少数为寄生菌，能引起人和植物的病害，有的酵母菌可与昆虫共生。Fermenter System for P. pastoris culture.The fermenter is supplied in a configuration to provide an excellent bioreactor for this purpose. * Powerful, top drive system with lubricated mechanical seal (magnetic options for Techfors) * Jacketed system for efficient heat transfer. * High-accuracy pumps for addition of pH control reagents, antifoam and feed (option on some units) * Control of dissolved oxygen by stirrer speed as standard (air flow, pressure & oxygen, supplement options) * Optional analogue feed pump for variable speed additon * Analogue input/output for addition of external pumpsand sensros for eg. OD and MethanolVessels: All vessels are stainless steel (1.4435) with a dished base and top plate in several standard sizes. Temperature control can be by water circulation with either a heating element or steam injection into a pocket/exchanger.All vessels are with a mechanical seal drive (magnetic coupling for cells). The drive shaft has a simple and secure connection for the motor. The top plate is equipped with 10mm, PG13.5 and standard, rounded -thread 19mm ports which can be fitted with a septum and retaining collar to allow flaming of the port during inoculation etc. (number and type varies according to specific fermenter). Bottom ports are 25mm Ingold standard and numbers vary by size.The vessels are sterilized in situ (Techfors and Techfors S) with automatic sequencing. Reagent bottles and inlet pipes etc are usually autoclaved separately (options & alternatives, depending on size and configuration).. StirringThe fermenter has a powerful brushless motor with integral speed controller for speeds from 100 - 1500 rpm, depending on the vessel size and number of impellors. Powerful fluid cooled motors are standard for Techfors.x-DDC Control System:All fermenter models except Minifors incorporate the x-DDC (eXtended DDC microprocessor control) which uses a high-performance digital system. With the use of almost identical instrumentation, the step from Bench-scale to larger equipment is made considerably easier.The control panel is compact and is usually mounted above the base unit for clear visibility. The panel can be removed if required. Programming of parameters is via a splashproof keypad with a rotary knob for navigation and an LCD display. Communication is via a robust data-cable. For each fermenter, process information such as parameter set points, PID values etc are stored automatically on an interchangeable "memory card" . This allows changes in fermentation processes by fitting an alternative card or downloading a recipe without re-programming and the chance of errors plus an unlimited number of recipes to be stored.An "open frame" gas supply system with four magnetic valves allow addition of components for automatic mixing of gasses for oxygen supplementation. An optional mass flow control valve ensures a controlled, steady flow of gas into the vessel. Agitation & temperature control. Four high-accuracy digital peristaltic pumps supplied as standard with the option to add an integral analogue feed pump. Addition and removal of liquids is therefore possible with a standard system (using the antifoam pump for medium take-off). Accepts a conductive probe for automatic control of antifoam addition or media addition/removal in a vessel. Works together with the integral peristaltic pumps.发酵罐在毕节酵母菌培养中的应用 分批反应操作是指基质灭菌、接种以后，除了好氧反应需要在反应过程中通入无菌空气、消除泡沫所用的消泡剂以及维持一定 pH 值所用酸碱之外，反应过程中不再加入反应基质。 基质浓度、产物浓度以及细胞浓度均随反应进行的时间而变化，尤其是菌体本身将经历不同的生长阶段(在培养的过程中，微生物的生长可分为迟缓期、对数生长期、减速期、静止期)，显示出不同的催化活力。 基本特征是：反应物料一次加入一次卸出；反应器物系的组成仅随时间而变化，即随着培养的进行，基质的浓度下降，菌体量增加，产物增加。因此属于一非稳态过程。 Fermenter System for P. pastoris culture. The fermenter is supplied in a configuration to provide an excellent bioreactor forthis purpose. * Powerful， top drive system with lubricated mechanical seal (magnetic options forTechfors) * Jacketed system for efficient heat transfer. * High-accuracy pumps for addition of pH control reagents， antifoam and feed(option on some units) * Control of dissolved oxygen by stirrer speed as standard (air flow， pressure &oxygen， supplement options) * Optional analogue feed pump for variable speed additon * Analogue input/output for addition of external pumpsand sensros for eg. OD and Methanol Vessels： All vessels are stainless steel (1.4435) with a dished base and top plate in severalstandard sizes. Temperature control can be by water circulation with either aheating element or steam injection into a pocket/exchanger. All vessels are with a mechanical seal drive (magnetic coupling for cells). The driveshaft has a simple and secure connection for the motor. The top plate is equippedwith 10mm，PG13.5 and standard， rounded -thread 19mm ports which can be fittedwith a septum and retaining collar to allow flaming of the port during inoculationetc. (number and type varies according to specific fermenter). Bottom ports are25mm Ingold standard and numbers vary by size. The vessels are sterilized in situ (Techfors and Techfors S) with automaticsequencing. Reagent bottles and inlet pipes etc are usually autoclaved separately(options & alternatives， depending on size and configuration).. Stirring The fermenter has a powerful brushless motor with integral speed controller forspeeds from 100-1500 rpm， depending on the vessel size and number of impellors.Powerful fluid cooled motors are standard for Techfors. x-DDC Control System： All fermenter models except Minifors incorporate the x-DDC (eXtended DDCmicroprocessor control) which uses a high-performance digital system. With theuse of almost identical instrumentation， the step from Bench-scale to largerequipment is made considerably easier. The control panel is compact and is usually mounted above the base unit for clear visibility. The panel can be removed if required. Programming of parameters is via asplashproof keypad with a rotary knob for navigation and an LCD display. Communication is via a robust data-cable. For each fermenter， process informationsuch as parameter set points， PID values etc are stored automatically on aninterchangeable "memory card". This allows changes in fermentation processes byfitting an alternative card or downloading a recipe without re-programming andthe chance of errors plus an unlimited number of recipes to be stored. An "open frame" gas supply system with four magnetic valves allow addition ofcomponents for automatic mixing of gasses for oxygen supplementation. Anoptional mass flow control valve ensures a controlled， steady flow of gas into thevessel. Agitation & temperature control. Four high-accuracy digital peristalticpumps supplied as standard with the option to add an integral analogue feedpump. Addition and removal of liquids is therefore possible with a standard system(using the antifoam pump for medium take-off). Accepts a conductive probe forautomatic control of antifoam addition or media addition/removal in a vessel.Works together with the integral peristaltic pumps.