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Application Note CD-0007 One-drop CD spectra measurement in using of Micro Sampling Disc Introduction CD spectroscopy is one of indispensable measurement method for protein structure analysis as same asNMR or X-ray crystallography. And small volume or low concentration sample measurement is commonrequest in this market recently. Jasco has developed new technology and application to meet such small-volume or low-concentration sample measurement condition finally. In this note， we like to show several measurement results by One-drop CD spectra measurements in usingof Micro sampling disk (MSD) Keywords： One-drop measurement， Protein， Circular Dichroism Fig. 1 MSD components 1. Add the sample to theMSD 2. Clamp the MSD 3.Place the MSD in thesample chamber Fig. 2 How to use the MSD Advantages 1. One-drop CD measurement 10 uL (1 mm path length)， 2 uL (0.2 mm path length)2. Easy handling Hydrophobic treatment keeps samples centered 3. Variable path length Spacers are attached for 1 or 0.2 mm path length4. Artifact free Windows allow for artifact-free measurements 5. Alignment free JASCO CD spectrometers use a parallel light beam 6. Highly reproducible baseline CD-0007 Results The following are comparisons between the MSD and a conventional cell. CD spectra that showsecondary structures (between 260 nm and 190 nm) can be measured completely in less than 3 minutes usingthe MSD. Fig.3 Hemoglobin Fig. 4 Lysozyme Fig .5 Concanavalin A Fig. 6 Trypsin Inhibitor MSD： Conventional oil： Measurement parameters Path length： 1 mmSample concentration： 0.1 mg/mLBandwidth：1 nmData interval： 0.1 nmScan speed： 100 nm/minResponse： 2 sec No of scans： 4 times (MSD)， 1 time (Conventional cell) Measurement time： 2.8 min (MSD)， 42sec (Conventional cell) copyrightOJASCO CorporationJASCO INTERNATIONAL CO.， LTD. -Sennin-cho -chome， Hachioji， Tokyo JapanTel：+ Fax： + CD spectroscopy is one of indispensable measurement method for protein structure analysis as same asNMR or X-ray crystallography. And small volume or low concentration sample measurement is common