牙齿中牙齿腐烂的定量评估检测方案(分子荧光光谱)

智能文字提取功能测试中

EMISSION·FLUORESCENCE·FORENSICS·GRATINGSFOEM·RAMAN·SPECTROSCOPIC SYSTEMS·THIN FILMS Characterizing Tooth Decay with Fluorescence Introduction Quick and quantitative analytical evaluationof tooth decay is required in any study of the en-vironmental factors promoting such decay. Thisinformation is essential to any study of whichdental products are most effective in slowing orarresting the decay. Teeth are naturally fluorescent solids， and arehighly-scattering samples that can introduce in-terference that would mask fluorescence indica-tors. Thus， a SPEXQ FLUOROLOGR spectro-fluorometer was selected， taking advantage ofdouble monochromators for ultra-pure spectra.Use of a FLUOROLOGQ allowed picosecondtime-discrimination via the FLUOROLOGQ-Taulifetime accessory for even more informationabout the samples. Results Fig.1 shows a contour plot of the excitation-emission matrix of the sample. The plot resultsfrom scanning the fluorescence at various excita-tion wavelengths. The clean enamel surface ex-hibited two large fluorescence bands at 430 and470 nm. Fig. 2 shows a surface heavily damaged bydecay. Though the 430- and 470-nm peaks werestill present， their relative intensities were re-versed. In addition， a new peak surfaced at 650nm. Lifetime data from the FLUOROLOGR-Tauconfirmed that there was indeed a third compo-nent present at 650 nm， with a long lifetime ofabout 600 ns. Conclusion The FLUOROLOGRsystem providesquantitative assessment of tooth decay， via the FLUOROLOGQ's exclusive strong stray-lightrejection， and ability to detect IR fluorescence. The FLUOROLOGQ-Tau accessory pro-vides an additional dimension of discrimination.With these data， new dental treatments and prod-ucts may be assessed. Further work may lead to aquick， convenient method of tracing decay. Figure 1. Clean enamel area of tooth Figure 2. Heavy decay area on tooth. Copyright@ 2000 Jobin Yvon Inc. In France：Jobin Yvon S.A.16-18， rue du Canal91165 Longjumeau cedexTel： (33) 1/64.54.13.00Italy：2/57.60.30.50Fax： (33) 1/69.09.93.19 Germany： 89/46.23.17-0 E-Mail： fluorescence@jyhoriba.com The FLUOROLOG® system provides a quantitative assessment of tooth decay, via the FLUOROLOG®’s exclusive strong stray-light rejection, and ability to detect IR fluorescence.The FLUOROLOG®-Tau accessory provides an additional dimension of discrimination. With these data, new dental treatments and products may be assessed. Further work may lead to a quick, convenient method of tracing decay.