尿液、尿样、组织样品中白蛋白检测方案(毛细管电泳仪)

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HIGH PERFORMANCECAPILLARYELECTROPHORESIS SYSTEMCAPELseriesWWW.LUMEX.BIZ DETERMNATION OF ALBUMIN CONCENTRATION IN URINEa (DIAGNOSTICS OF MICROALBUMINURIA) INTRODUCTION The capillary electrophoresis method provides determination of albumin concentration in urine. MEASUREMENT METHOD Determination of albumin is based on electrophoretic migration of its anionic form in the electric field and itsdirect detection by measuring the UV absorption in the 215-220-nm range. Prior to analysis sample aliquotwas desalted either by ultra-diafiltration or on the“Sephadex G-25”column. REFERENCE CONCENTRATION OF ALBUMIN IN URINE The albumin content in urine of a healthy person is less than 20 mg/dm°. EQUIPMENT AND REAGENTS The “CAPEL-105/105M”capillary electrophoresis system is used in measurements. Data acquisition， collection， processing and output are performed using a personal computer running under“WINDOWS° 2000/XP”operating system with installed dedicated software package for acquisition andprocessing of chromatography data. All reagents must be of analytical grade or higher. EXAMPLES OF REAL ANALYSES Buffer： borate， with SDS， pH 9.2Capillary： Leff/ Ltot 50/60 cm， ID 75 pmInjection： 450 mbar x sec Voltage： +15 kV Detection： 215nm Sample： urine of a healthy personMeasurement results： detectedalbumin concentration 13 mg/dm° Sample： urine of a patient with diabetesMeasurement results： detectedalbumin concentration 117 mg/dm° The contents on this paper are subject to change without notice.     微量白蛋白尿是反映肾小球疾病和损伤的一个非常灵敏的指标，在预示糖尿病肾病方面已使用多年。近年有资料显示，微量白蛋白尿也是非糖尿病患者心血管疾病发病的一个危险因子，收到广泛重视。因此尿液中白蛋白的准确测定就就显得很重要。    毛细管电泳法测定尿液中的白蛋白是基于其阴离子形式在电场中的电泳迁移并通过测量215～220 nm范围内的紫外吸光度而直接检测。在分析之前，样品需要通过超滤膜脱盐或在“Sephadex®G-25”柱上进行脱盐处理。LUMEX鲁美科思毛细管电泳CAPEL高效快速准确的测定尿样中白蛋白含量。