饮料、果蔬制品、蜂蜜和生物活性食品添加剂中果糖、葡萄糖和蔗糖检测方案(毛细管电泳仪)

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HIGH PERFORMANCECAPILLARYELECTROPHORESIS SYSTEMWWW.LUMEX.BIZ DETERMINATION OF FRUCTOSE， GLUCOSE， AND SACCHAROSE INBEVERAGES， FRUIT AND VEGETABLE PRODUCTS， HONEY， ANDBIOACTIVE FOOD ADDITIVES LUMEX Method M 04-69 (2011) INTRODUCTION The technical directives and regulatory requirements specify the total content and relative contents offructose， glucose and saccharose in juices， nonalcoholic and alcoholic beverages (incl. wines andwinemaking materials)， fruit and vegetable products， honey，and bioactive food additives. MEASUREMENT METHOD The method is based on dilution of liquid samples with distilled water or extraction of saccharose-likecompounds from solid samples by distilled water and subsequent quantitative determination ofconcentration of the analyzed components by capillary electrophoresis. The components are indirectlydetected by measuring their absorbance at a wavelength of 254 nm. MEASUREMENT RANGE The measurement range of the concentration of the components is 2-800 g/l (0.2-80%). EQUIPMENT AND REAGENTS A CAPEL-105/105M capillary electrophoresis system with a special capillary cassette for the sugar analysis is used in measurements.Data acquisition， collection， processing and output are performed using a personal computer runningunder WINDOWS° 2000/XP operating system with installed dedicated software package for acquisitionand processing of chromatography data. All reagents must be of analytical grade or higher. EXAMPLE OF A REAL ANALYSIS Separation conditions： Buffer：electrolyte based on potassium sorbate with CTAB (pH 12.1)Capillary：Leff/ Ltot =65/75 cm， ID= 50 pmSample injection：150 mbar*secVoltage：-25 kVTemperature：20CDetection：254 nm 4.0 mAU The contents of this paper are subject to change without notice. LUMEX成功的将毛细管电泳发展成为实验室的常规分析手段，并一直占据该领域的市场领导地位。成熟的仪器、优化的配置、大量的应用方法包集于一体，被用户称为目前性价比最优的分析仪器。相关的技术指令和法规要求规定了果汁、非酒精和酒精饮料（包括葡萄酒和酿酒原料）、水果和蔬菜制品、蜂蜜和生物活性食品添加剂中果糖、葡萄糖和蔗糖的总含量和相对含量。毛细管电泳法的分析优势特点有：1.高效分离效率(百万级理论塔板数)；2.样品试剂和样品量消耗低；3.极低的分析成本，毛细管柱一般无需更换；4.操作简单便捷，实现快速分析 (5-10分钟)。