黄原胶中黏度检测方案(粘度计)

智能文字提取功能测试中

黄原胶溶液黏度的测定及流动曲线的建立 应用资料黄原胶是一种多糖，由玉米等淀粉与细菌发酵制成。由于黄原胶与水混合时具有粘性，因此在食品中用作增稠剂和增稠稳定剂，用于调味品、各种酱料、蒸煮袋食品和冷冻食品等，用途广泛。使用EMS黏度计测量含有黄原胶的溶液粘度的变化，此黏度计可通过密封、灭菌和非接触测量。京都電子工業株式会社KYOTO ELECTRONICSAPVS-0113enMANUFACTURING CO.，LTD. APVS-0113en6. Procedure Application Note Measurement of viscosity of xanthan gum solutionand creation of flow curve Industry Food & beverage， Cosmetics & soap， Pharmaceutical Instrument Viscometer Measurement method ： Electro Magnetically Spinning Method Standards GB 1886.41 1. Scope Xanthan gum is one of polysaccharides made by fermenting starch such as corn with bacteria(Xanthomonas campestris). Since xanthan gum is viscous when mixed with water， it is used in foods as a thickener andthickening stabilizer for dressing， various sauces， retort pouch foods and frozen foods etc. for awide range of applications. An example of measuring Change in viscosity of the solution， containing xanthan gum， using anEMS viscometer that can be measured by sealing， sterilization and non-contact will be shownbelow. 2. Precautions Although xanthan gum is soluble in water， it does not dissolve immediately after adding ionexchanged water， so it is necessary to sufficiently stir using Vortex etc. after establishing asufficient swelling time.In this measurement example， ion-exchanged water was added to xanthan gum weighed so as tohave each concentration， and then allowed to stand at room temperature for about 14 hours ormore to swell. 3.Post-measurement procedure The sample container and the sample are discarded appropriately. 4. Apparatus EMS Viscometer Control Laptop PC 5. Reagents ·Sample ：0.1%，0.2%，0.3% xanthan gum(Food additive grade) solution·Ion exchanged water 1) Enter the following conditions in measurement condition of the sequence mode of controsoftware. Measurement mode ：Repeat mode， Sequence mode Measurement temperature ：25℃ Motor rotation speed ：I (1，000rpm ： repeat mode)II(100~1，000rpm ： sequence mode：Rise and descent in increments of 100 rpm)Measurement time ：I (1 second)~III(10 seconds)Repeat count ：I (5 times ： repeat mode I(5 times sequence mode) Measurement interval ：5 seconds VWaiting time for temperature stability ：5 minutes 2) Place an aluminum spherical probe of o 2 mm and a sample of 300 pL in a container， coverwith a cap and packing， set the sample container in the EMS Viscometer， and click themeasurement button. 3) Measure another samples on the same condition after the measurement of the first sample iscompleted. 7.Example The measurement results of viscosity of each concentration of xanthan gum solution wereshown in Figure 1， Table 1， flow curve of 0.1% xanthan gum solution was shown in Figure 2，Table 2(measurement results)， and Casson plot was shown in Figure 3. Although the coefficient of variation (CV) in the viscosity measurement of 5 times (n =5) of0.1%， 0.2%， 0.3% xanthan gum solution tends to increase as the concentration increases， in anysample 0.5% or less It was stable measurement result. For reference， it was possible to measure the viscosity in about 6 minutes， including 5 minutesof temperature stabilization wait time by repeating measurement (5 times). In the sequencingmeasurement， it was possible to create a flow curve in about 40 minutes. Figure 1. Measurement viscosity of xanthan gum solutions Table1. Measurement results of xanthan gum solutions Concentration of xanthan gum solution(%) 0.1 0.2 0.3 Motor rotation speedrpm n Viscosity(mPa"s) Shear rates-1 Viscosity(mPa's) Shear rates-1 Viscosity(mPa's) Shear rates-1 1000 1 19.1 52.94 57.5 22.93 134 10.71 2 19.1 52.78 57.4 22.93 134 10.71 3 19.1 52.78 57.6 22.78 135 10.68 4 19.1 52.78 57.7 22.78 135 10.68 5 19.1 52.78 57.5 22.93 135 10.65 Ave 19.1 52.81 57.54 22.87 134.6 10.69 SD 0.000 0.070 0.114 0.086 0.548 0.026 CV 0.0% 0.1% 0.2% 0.4% 0.4% 0.2% Figure 2. Flow curve of 0.1% xanthan gum solution Figure 3.。Casson plot of 0.1% xanthan gum solution Table2. Measurement results of 0.1% xanthan gum solution Motor rotaion speedrpm Measurement times Ave(mPa's) SD CV 1 2 3 4 5 Rise 100 210.0 209.0 210.0 210.0 211.0 210.0 0.707 0.3% 200 106.0 109.0 110.0 108.0 108.0 108.2 1.483 1.4% 300 69.9 69.7 70.0 70.1 70.1 70.0 0.167 0.2% 400 50.9 50.8 51.0 50.8 51.1 50.9 0.130 0.3% 500 41.0 41.2 41.0 41.6 41.0 41.2 0.261 0.6% 600 32.5 32.9 32.7 32.7 33.0 32.8 0.195 0.6% 700 29.1 29.1 29.1 29.0 29.0 29.1 0.055 0.2% 800 25.7 25.7 25.8 25.7 25.7 25.7 0.045 0.2% 900 23.3 23.3 23.2 23.3 23.2 23.3 0.055 0.2% 1000 21.1 21.1 21.1 21.2 21.3 21.2 0.089 0.4% Descent 900 23.4 23.3 23.3 23.3 23.2 23.3 0.071 0.3% 800 25.0 25.1 25.0 25.0 24.9 25.0 0.071 0.3% 700 28.4 27.9 27.3 27.9 27.9 27.9 0.390 1.4% 600 31.8 31.8 31.7 31.6 31.9 31.8 0.114 0.4% 500 38.6 38.7 38.7 39.0 38.7 38.7 0.152 0.4% 400 49.5 49.5 49.4 49.7 49.7 49.6 0.134 0.3% 300 66.7 66.6 66.4 66.3 66.2 66.4 0.207 0.3% 200 100.0 101.0 101.0 102.0 102.0 101.2 0.837 0.8% 100 235.0 228.0 232.0 228.0 227.0 230.0 3.391 1.5% 8. Summary The viscosity of 5 times (n=5) of 0.1%， 0.2%，0.3% xanthan gum solutions could be measuredin about 6 minutes， and the coefficient of variation (CV) at that time was an accuracy of 0.5% orIess. In the measurement by the sequence mode， it was confirmed that flow curve could be createdwithout breaking the higher-order structure of sample molecule， because the shear rate increasedand the descending viscosity were almost the same. 9. References None. 京都电子工业株式会社-可睦电子(上海)商贸有限公司电话： 021-54488867 电邮： kemu-kem@163.com 都電子工業株式会社KYOTO ELECTRONICSMANUFACTURING CO.，LTD