蛋白质溶液中粘度检测方案(粘度计)

智能文字提取功能测试中

蛋白质溶液中粘度的测定 应用资料白蛋白是制药工业中应用最广泛的蛋白质之一。牛血清白蛋白(BSA)是生产治疗药物和作为酶稳定剂等的体外诊断剂的必要成分。使用EMS粘度计测量蛋白质溶液动态粘度的浓度依赖性应用资料，该粘度计可以通过密封、灭菌和非接触来测量。京都電子工業株式会社KYOTO ELECTRONICSAPVS-0109en-1MANUFACTURING CO.，LTD. APVS-0109en-16. Procedure Application Note Concentration dependence of viscosity of protein solutions IndustryPharmaceutical， Food & beverageInstrumentViscometerMeasurement method：Electro Magnetically Spinning MethodStandards 1.Scope Albumin is one of the most widely used proteins in the pharmaceutical industry. Bovine Serum Albumin (BSA) is an essential ingredient for the production of therapeutic drugsand extracorporeal diagnostic agents as enzyme stabilizers and the like. Examples of measuring the concentration dependency of the dynamic viscosity of proteinsolutions using an EMS viscometer that can be measured by sealing， sterilization andnon-contact were shown below. 2. Precautions None. 3.Post-measurement procedure The sample container and the sample are discarded appropriately. 4. Apparatus EMS Viscometer Control Laptop PC 5. Reagents ·Sample ： Bovine serum albumin， ovalbumin， ·Phosphate Buffered Saline (PBS(-)) solution 京都电子工业株式会社-可睦电子(上海)商贸有限公司 上海市徐汇区宜山路333号1201室电话：021-54488867 电邮： kemu-kem@163.com 网址： http：//www.kem-china.com 1) Enter the following conditions in measurement condition of the sequence mode of controlsoftware. Measurement mode ：Sequence mode Measurement temperature ：25℃ Motor rotation speed ：1，000rpm Measurement time ：I (1 second) Repeat count ：5 times Measurement interval ：5 seconds VWaiting time for temperature stability ：5 minutes 2) Place an aluminum spherical probe of p 2 mm and a sample of 300 pL in a container， coverwith a cap and packing， set the sample container in the EMS Viscometer， and click themeasurement button. 3) Add the dilution solvent after the first measurement is completed， and measure in sequence underthe same condition. 7.Example The concentration dependence of viscosity of bovine serum albumin and ovalbumin both can beconfirmed. The measurement results are shown in Figure 1 and Table 1， 2. The viscosity ofbovine serum albumin solutions is obtained higher value compared with that of ovalbuminsolutions at high concentrations of 250 mg/mL or more， though a significant difference ofviscosity between two kinds of protein solutions at low concentrations is not observed. Figure 1. Measurement result about concentration dependence of viscosity of protein solutions Table 1. Concentration dependence of viscosity of bovine serum albumin (mPa's) Concentration (mg/mL) 50 100 150 200 250 300 350 1st 1.53 1.61 2.31 2.82 4.27 6.56 11.30 2nd 1.53 1.61 2.31 2.82 4.26 6.55 11.40 3rd 1.53 1.61 2.31 2.81 4.26 6.57 11.30 4th 1.54 1.62 2.29 2.82 4.27 6.63 11.30 5th 1.53 1.62 2.33 2.82 4.27 6.57 11.30 Average 1.53 1.61 2.31 2.82 4.27 6.58 11.32 Standard deviation 0.00 0.01 0.01 0.00 0.01 0.03 0.04 RSD 0.3% 0.3% 0.6% 0.2% 0.1% 0.5% 0.4% Table 2. Concentration dependence of viscosity of ovalbumin (mPa's) Concentration (mg/mL) 50 100 150 200 250 300 350 lst 1.30 1.60 1.94 2.79 3.72 5.96 8.35 2nd 1.29 1.60 1.92 2.73 3.73 5.98 8.34 3rd 1.29 1.60 1.92 2.73 3.73 6.04 8.38 4th 1.30 1.60 1.92 2.75 3.73 5.98 8.42 5th 1.29 1.59 1.92 2.75 3.73 5.99 8.32 Average 1.29 1.60 1.92 2.75 3.73 5.99 8.36 Standard deviation 0.01 0.00 0.01 0.03 0.00 0.03 0.04 RSD 0.4% 0.3% 0.5% 1.0% 0.1% 0.5% 0.5% 8. Summary Even if it is a rare sample (expensive sample) such as the protein， it is possible to evaluate theconcentration dependence with a small amount of sample by measuring while adding thedilution solvent and diluting. 9. References None. 京都电子工业株式会社-可睦电子(上海)商贸有限公司电话：021-54488867 电邮： kemu-kem@163.com 都電子工業株式会社KYOTO ELECTRONICSMANUFACTURING CO.，LTD