sslin
第1楼2006/03/09
我的阅读要点摘录:
1). average 1JCH, usually 14O Hz, but if there are no aromatic or alkene groups, then 125 Hz may be a better choice
2). HMBC is the equivalent of LRHETCOR but more sensitive
3). 2JCH is so small that a crosspeak may not be observed but 3JCH is large resulting in an intense crosspeak
4). The spectrum below demonstrates several aspects of HMBC. Firstly, not all 2,3 and 4 bond correlations are observed
celan
第2楼2006/03/09
哈,sslin老师的确把这个短文的精华摘出来了.
另外,有时后即使S/N很好,有的重要相关峰还是出不来,影响结构鉴定.我在做一个小肽的序列时就遇到这种情况,这时候请考虑下面的参数.
The delay corresponding to the multiple bond J coupling nJCH (taumb) is entered directly in seconds (a good first choice is O.04 sec , but if the long range couplings are small then O.O6 sec is optimum).
但愿上述讨论能对有些朋友有用.