guojtjt
第1楼2007/01/12
高效液相色谱法测定柑橘汁中的柠檬苦素和柚皮苷陈静[1] 高彦祥[1] 吴伟莉[2] 李绍振[2][1]中国农业大学食品科学与营养工程学院,北京100083 [2]北京汇源饮料食品有限责任公司,北京101305摘 要:柑橘汁的苦味主要是由于柚皮苷和柠檬苦索的存在所致,其含量的测定可用于控制柑橘类果汁的质量。采用高效液相色谱法在KR100-5C18(4.6mm i.d.×250mm,5μm)上,分别以乙腈-四氢呋喃-水(体积比为17.5:17.5:65)和甲醇-冰醋酸-水(体积比为40:1:59)为流动相(流速均为1mL/min),在207nm和283nm检测波长下分别测定了柠檬苦素和柚皮苷。实验结果表明,柠檬苦素在1.00~50.00mg/L时线性关系良好(r=0.9992),检出限为0.07μg,平均加标回收率为98.69%,相对标准偏差(RSD)为2.5%;柚皮苷在20.00~160.00mg/L时线性关系良好(r=0.9988),检出限为0.14μg,平均加标回收率为100.13%,RSD为1.5%。用该法检测柑橘汁样品中的柠檬苦素与柚皮苷,方法简便、快速、准确。[著者文摘]
关键词:高效液相色谱法 柠檬苦素 柚皮苷 柑橘汁
分类号: O658[著者标引]文献标识码:A文章编号:1000-8713(2006)02-0157-04相关文献:主题相关 参考文献 引用本文
Determination of Bitter Components in Citrus Juice by High Performance Liquid ChromatographyCHEN Jing, GAO Yanxiang, WU Weili, LI Shaozhen (1. College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2. Huiyuan Beverage & Food Groub Co., Ltd, Beijing 101305, China)Abstract:Bitterness in citrus juice is primarily due to the presence of both limonin and naringin, and determination of them is a standard in controlling the quality of juice. A high performance liquid chromatographic (HPLC) method was developed for the rapid determination of bitter components in citrus juice. The HPLC conditions were as follows: ODS column with acetonitrile-tetrahydrofuran-water (17.5 : 17.5 : 65, v/v) as mobile phase, flow rate at 1 mL/min, 207 nm as detection wavelength for limonin, and methanol-100% acetic acid-water (40 : 1 : 59, v/v) as mobile phase, flow rate at 1 mL/min, 283 nm as detection wavelength for naringin. As results showed, for limonin and naringin, the linearity was good within the ranges of 1.00 - 50.00 mg/L (r = 0.999 2) and 20.00 - 160.00 mg/L (r = 0.998 8) respectively, and the detection limits were 0.07μg and 0.14μg, then the average recoveries were 98.69% and 100.13% with relative standard deviations of 2.5% and 1.5%, respectively. The method is simple, rapid and accurate for determining limonin and naringin in juice.[著者文摘]
Key words:high performance liquid chromatography (HPLC
我在故我思
第5楼2007/01/15
HPLC法同时测定吴茱萸中吴茱萸苦素和柠檬苦素的含量英文
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甜橙种子中柠檬苦素类似物配糖体的测定
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什么都不懂
第6楼2007/01/16
太强了,我以前也下载了你很多上传的资料,谢谢了