产品介绍
MAbPac Protein A LC 色谱柱
Thermo Scientific? MAbPac? Protein A LC 色谱柱用于收集细胞培养物,可以实现快速而准确的单克隆抗体 (MAb) 滴度分析。 该色谱柱将低反压和高效的优点相结合,可以实现高通量和高准确性的分析。 独特的规格使其适用于生物相容系统,可以快速而便捷的实现加载、结合、淋洗和收集自动化。 该色谱柱为非多孔、聚合物树脂,具有疏水的二乙烯基苯核及亲水表面,专为亲和分离而优化,具有出色的稳健性和可重现性。
快速而准确的 MAb 滴度分析
完整循环时间(包括平衡)仅 2 分钟
较宽的浓度线性范围 (0.025 - 5 mg/mL),无残留效应
柱压低,在 2mL/min 流速下柱压低于 250 psi
出色的样品回收率
MAbPac Protein A LC 色谱柱可用于滴度分析或浓缩与纯化 IgG 材料,以供后续实验或二维 (2D) 色谱分析使用。 该色谱柱规格为 HPLC 生物惰性、4×35mm PEEK 柱身。
MAbPac Protein A LC 色谱柱
订货信息:
MAbPac Protein A, 12 μm, Analytical Column (4 x 35mm) | 082539 |
MAbPac Protein A Column for Monoclonal Antibody Titer Analysis
The Thermo Scientific? MAbPac? Protein A column is a high-performance, affinity chromatography column specifically designed for fast, accurate determination of monoclonal antibody (MAb) concentration in the harvest cell culture (HCC). Early in the development of recombinant MAbs, a large of number of HCC samples must be screened for IgG titer. Affinity chromatography employing a Protein A ligand is often used to determine the MAb concentration. The MAbPac Protein A column is a high-pressure liquid chromatography (HPLC) column designed to provide fast, accurate, titer analysis with linearity over a wide concentration range without carryover.
The MAbPac Protein A column is based on a novel nonporous polymeric resin consisting of a hydrophobic divinylbenzene core and a hydrophilic surface. This proprietary resin is designed for fast mass transfer, resulting in efficient separations without carryover. The recombinant Protein A ligands are covalently attached onto the hydrophilic resin surface through their amine groups to provide a very rugged, reproducible column. The column is available in a HPLC bioinert, 4 × 35 mm PEEK column body.
Product Highlights:
? A novel nonporous polymeric resin with a hydrophilic surface
?
Functionalized with the recombinant protein A ligands
? Wide range of MAb
quantitation: 0.025–5 mg/mL
? Fast analysis: 2 min/analysis
? Rugged
?
Low pressure: column pressure is less than 250 psi at 2 mL/min
The MAbPac Protein A column can be used for both titer analysis or concentrating and purifying IgG material for later experiments or two-dimensional (2D) chromatography.
MAbPac Protein A Column Specifications | |
---|---|
Column Dimension | 4 × 35 mm |
Base Matrix Material | Hydrophilic nonporous resin |
Surface Ligand | Protein A |
Particle Size | 12 μm |
Binding capacity | ~3.5 mg IgG/g resin |
Dynamic Loading Capacity | ≤100 μg IgG/column at 2 mL/min flow rate |
pH Range | 1.9–7.5 |
Maximum Flow rate | Up to 2 mL/min |
Maximum Pressure | ≤1000 psi |
Operating Temperature | <30 ?C |
Data Sheets | |
---|---|
Thermo Scientific MAbPac Protein A Column |
Manuals | |
---|---|
Thermo Scientific MAbPac Protein A Column Quickstart | |
Thermo Scientific MAbPac Protein A Column Product Manual |
Posters | |
---|---|
Protein A Affinity Column for Monoclonal Antibody (MAb) Titer Analysis |
Applications | |
---|---|
A Novel Affinity Protein A Column for Monoclonal Antibody (MAb) Titer Analysis |
Brochures | |
---|---|
Thermo Scientific BioLC Columns |
供应商
银牌会员 第9年