Dionex CarboPac PA200 碳水化合物分析柱

Dionex CarboPac PA200 碳水化合物分析柱

品牌戴安

货号62896 价格  面议

产品介绍

Dionex CarboPac PA200 碳水化合物分析柱



对于高分辨率测绘和糖蛋白释放的寡糖的分析,使用的Thermo Scientific戴安CARBOPACPA200分析及保护柱提供的敏感性和特异性检测。脉冲安培检测(PAD)相结合,此阴离子交换柱提供无需使用衍生快速,重复分离。疏水性聚合物,薄壳型阴离子交换树脂填充材料是在pH值0-14的范围内保持稳定。这种独特的pH值稳定性允许使用有利于碳水化合物的阳极氧化的金电极洗脱液组成的。



Dionex CarboPac PA200 碳水化合物分析柱

订货信息:

分析柱
CarboPac PA200 Analytical Column (3 x 250 mm)062896
保护柱
CarboPac PA200 Guard Column (3 x 50 mm)062895



Fast, pH-Stable Separation of Oligosaccharides

A variety of HPLC approaches are proposed for characterizing oligosaccharides, but many prove inadequate for separating complex mixtures and are limited by a lack of specificity and high limits of detection. Dionex has developed an improved HPLC technique for carbohydrate analysis based on anion-exchange chromatography coupled with pulsed amperometric detection. This technique provides high-resolution separations with sensitive and specific detection, without derivatization.

  • Sensitive, direct detection with no derivatization, using pulsed amperometric detection

  • Very high-efficiency separations

  • Separations based on size, charge, degree of branching, and linkage isomerism

  • Low flow rate saves on eluent consumption

  • Does not require derivatization

CarboPac? PA200 columns are packed with a hydrophobic, polymeric, pellicular anion-exchange resin stable over the range of pH 0–14. The unique pH-stability of the packing material allows the use of eluent compositions that are conducive to anodic oxidation of carbohydrates at gold electrodes.

 

 

CarboPac PA200 Column for High-Resolution Oligosaccharide Separations Specifications
DimensionsCarboPac? PA200 Analytical Column:
3 × 250 mm
CarboPac PA200 Guard Column:
3 × 50 mm
Maximum operating pressure4000 psi (27.9 MPa)
Temperature range6–60 °C  
Recommended operating temperature: controlled ambient
Typical operating conditions2700 psi at 0.5 mL/min (analytical and guard columns)
Recommended flow rate: 0.5 mL/min
Mobile phase compatibilitypH 0–14; 100% compatible with common organic solvents. 
Ionic form eluents: sodium acetate and sodium hydroxide only.
Resin composition5.5 μm-diameter ethylvinylbenzene/divinylbenzene substrate (55% cross-linked) agglomerated with 43 nm MicroBead? quaternary amine functionalized latex (6% crosslinked)
Anion-exchange capacityApproximately 35 μeq/column (3 × 250 mm analytical column)
Column constructionPEEK? with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic
Product Data Sheets
CarboPac PA200 Column Solutions for Oligosaccharide Analysis Data Sheet
Application Updates
AU 150: Determination of Plant-Derived Neutral Oligo- and Polysaccharides Using the CarboPac PA200
AU 176: Preparation of Peptide N-Glycosidase F Digests for HPAE-PAD Analysis
Application Notes
AN 1050: Evaluating Protein Glycosylation in Limited-Quantity Samples by HPAE-PAD
AN 1013: Polysialic Acid Analysis: Separating Polymers with High Degrees of Polymerization
AN 155: Determination of Trans-Galactooligosaccharides in Foods by AOAC Method 2001.02
AN 147: Determination of Polydextrose in Foods by AOAC Method 2000.11
AN 105: Glycosylation Analysis of Human Serum Transferrin Glycoforms Using Pellicular Anion-Exchange Chromatography
AN 67: Determination of Plant-Derived Neutral Oligo- and Polysaccharides
AN 46: Ion Chromatography: A Versatile Technique for the Analysis of Beer
Technical Notes
TN 42: Glycoprotein Oligosaccharide Analysis Using High-Performance Anion-Exchange Chromatography
TN 20: Analysis of Carbohydrates by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD)
Manuals
CarboPac PA200 Analytical Column Manual




Dionex CarboPac PA200 碳水化合物分析柱信息由北京豫维科技有限公司为您提供,如您想了解更多关于Dionex CarboPac PA200 碳水化合物分析柱报价、型号、参数等信息,欢迎来电或留言咨询。除供应Dionex CarboPac PA200 碳水化合物分析柱外,北京豫维科技有限公司还可为您提供PharMed 泵管(橙色/黄色) 6.1826.420Clarus气相色谱系统的Swafer工具包和附件UPELCOSIL LC-NH2 Supelguard 柱芯 59558等产品,公司有专业的客户服务团队,是您值得信赖的合作伙伴。

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