产品介绍
DNAPac PA200 寡核苷酸柱
使用 Thermo Scientific DNAPac PA200 寡核苷酸柱,可实现对合成型寡核苷酸进行高分离度分析和纯化所需的强阴离子交换。
可对寡核苷酸进行 n、n-1 分离
解析次级结构的寡核苷酸
分析硫代磷酸酯纯度
通过调节洗脱液 pH、盐和溶剂来进行选择性控制
可解析具有不同于正常 SSRNA 的异常链 (2' – 5') 的 RNA
DNAPac PA200 寡核苷酸柱
订货信息:
RSLC 分析柱 | |
DNAPac PA200 RS, 4μm,Fast Analytical Column (4.6 x 50 mm) | 082508 |
DNAPac PA200 RS, 4μm, Analytical (4.6 x 150 mm) | 082509 |
DNAPac PA200 RS, 4μm,High Resolution Analytical Column (4.6 x 250 mm) | 082510 |
分析柱 | |
DNAPac PA200, 8μm, Analytical (2 x 250 mm) | 063425 |
DNAPac PA200, 8μm, Analytical Column (4 x 250 mm) | 063000 |
DNAPac PA200, 8μm, Analytical (9 x 250 mm) | 063421 |
DNAPac PA200, 8μm, Semi-Preparative (22 x 250 mm) | SP6734 |
保护柱 | |
DNAPac PA200, 8μm, Guard (2 x 50 mm) | 063423 |
DNAPac PA200, 8μm, Guard Column (4 x 50 mm) | 062998 |
DNAPac PA200, 8μm, Guard (9 x 50 mm) | 063419 |
DNAPac PA200, 8μm, Guard (22 x 50 mm) | SP6731 |
The Thermo Scientific™ DNAPac™ PA200 and PA200 RS are strong anion-exchange columns developed to provide unsurpassed high-resolution analysis and purification of synthetic oligonucleotides. Both supports are designed to resolve full length from n–1, n+1, and other failure sequences not possible with other columns. Retention times and selectivity can be controlled by choice of salt, pH, and solvent. Therefore, the separation can be tailored to the requirements of many different oligonucleotide analysis challenges.
Achieve n, n-1 resolution for oligonucleotides.
Resolve oligonuleotide linkage and stereoisomers.
Assay phosphorothioate purity.
Attain selectivity control with eluent pH, salt, and solvent.
Resolve RNA with aberrant (2’–5’) links from normal SSRNA.
The DNAPac PA200 is packed with a pellicular anion-exchange resin composed of an 8 μm diameter nonporous polymeric substrate to which quaternary amine-functionalized Thermo Scientific™ Dionex™ MicroBeads™ are bound. The rapid mass transport characteristics of this resin result in high-resolution oligonucleotide separations.
The DNAPac PA200 RS is smaller, with 4 μm diameter resin for bio-UHPLC separations. These rapid separation (RS) columns are designed for use with the Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS system, with an operational backpressure limit of 10,000 psi. Columns packed with smaller particles improve resolution and offer better performance.
The DNAPac PA200 and PA 200 RS can be operated under denaturing conditions, such as high temperature (up to 85 °C), high-pH eluents (up to pH 12.5), or by the inclusion of chaotropic agents (such as Urea).
These columns offer the highest-quality phase stability over a broad pH range, deliver exceptional resolution, and support high-throughput separations. DNAPac PA200 and PA 200 RS columns support resolution of normal-length oligonucleotides (8- to 30- mer), extended-length oligonucleotides (30- to 70-mer), linear double-stranded DNA, and supercoiled versus nicked/linear DNA.
Analytical separations on the DNAPac PA200, 8μm, 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. Scaling the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.
Column Specifications | ||
---|---|---|
DNAPac PA 200 | DNAPac PA 200 RS | |
Column Chemistry | 55% crosslinked nonporous polymer with Quaternary amine functionalized latex MicroBeads | |
Particle Size | 8 μm | 4 μm |
Ion Exchange Capacity | ~ 14 μeq/mL | ~ 20 μeq/mL |
pH Range | 4 to 10 (12.5 with salt, see manual) | |
Pressure Maximum | 4,000 psi | 10,000 psi |
Temperature range | Recommended operating temperature: Controlled ambient Temperature limit: 85 °C | |
Flow Rates | Recommend 0.1 to 1.5 mL/min | |
Organic Solvent Limit | 100% acetonitrile or methanol for cleaning | |
Typical Eluents | High purity water (18 megohm-cm), sodium chloride, sodium perchlorate, buffers, sodium acetate and sodium hydroxide. | |
Detergent Compatibility | Compatible with nonioinic, cationic or zwitterionic detergents. |
Resolution of oligonucleotides using DNAPac PA200 column (8μm).
Separation of oligonucleotides by length using DNAPac PA200 column (8μm).
Influence of pH on Oligonucleotide Retention: NaCl Eluent ±20% CH3CN.
Improved Stability to Alkaline Conditions: Comparison of Capacity upon Exposure to pH 12.4 at Elevated Temperature.
Improved oligonucleotide sensitivity using the 4μm DNAPac PA200 RS column.
Fast separation of 46 oligonucleotides using the 4μm, DNAPac PA200 RS column.
Comparison of 4μm, DNAPac PA200 RS column throughput.
Separation of three identical sequence oligonucleotides harboring zero, one, or two 2',5'-linkages using the 4μm, DNAPac PA200 RS columns of different lengths.
Product Data Sheets | |
---|---|
DNAPac PA200 Column Data Sheet |
Guides | |
---|---|
DNAPac PA200 Analytical Column Manual | |
DNAPac PA200 RS 4μm Columns Quick Start |
Articles | |
---|---|
High-Resolution Nucleic Acid Separations by High-Performance Liquid Chromatography |
问商家
供应商
银牌会员 第9年