产品介绍
Bio-Scale Mini Nuvia IMAC Cartridges are prepacked with Nuvia Ni2+-charged IMAC Resin for purification of recombinant histidine-tagged proteins.
Nuvia IMAC Resin, based on Nuvia Beads, contains nitrilotriacetic acid (NTA) as the chelating ligand for di- or trivalent metal ions. The chemical structure of NTA permits highly selective binding of recombinant histidine-tagged proteins when charged with Ni2+ or other transition metals, such as Zn2+ or Cu2+.
Double-walled Bio-Scale Mini Cartridges are convenient, ready to use, and disposable. These 5 ml cartridges are designed for use with a peristaltic pump, Biologic™ and NGC™ Chromatography Systems, or any chromatography system.
Features and Benefits of Nuvia IMAC Columns
Double-walled cartridge design for extra durability, for pressures up to 45 psi
High capacity, even at high flow rates
Stable at pH 2–14
Chemically compatible polypropylene parts
Reusable/disposable format with luer fittings for easy connection to any system
Applications of Nuvia IMAC Columns
For purifying biomolecules in a variety of workflows:
Excellent single-step purity via affinity chromatography
Quick, efficient separation of biomolecules
Contaminant removal
Robust base matrix
This IMAC column resin is compatible with 8 M urea, 6 M guanidine HCl, 1 M NaOH, alcohol up to 20% (v), and with common detergents, salts, reducing agents, and all commonly used aqueous buffers.
Related Products
Bio-Rad also offers Nuvia™ IMAC Resin for packing into the column of your choice.
25 ml (780-0800)
100 ml (780-0801)
500 ml (780-0802)
Bio-Rad also offers other low-pressure columns for affinity purification.
Fittings are available for connection to HPLC- and FPLC-type systems.
Luer to M6 Adaptor Fittings Kit (732-0111)
Luer to BioLogic™ and NGC™ Systems Fittings Kit (732-0113)
Bio-Rad chromatography systems:
BioLogic™ LP Low-Pressure Chromatography Systems
NGC™ Medium-Pressure Chromatography Systems
技术指标:
Size | 5 ml bed volume IMAC cartridges: |
Recommended flow rate | 7 ml/min |
Maximum operating pressure | 45 psi |
Fittings | Female luer inlet and male luer outlet |
Column material | Polypropylene |
Frit material | Polyethylene (HDPE) |
Dimensions (L x ID) | 40 x 12.6 mm |
订货信息:
Bio-Scale Mini Nuvia IMAC Cartridges
名称 | 规格 | 货号 |
Bio-Scale™ Mini Nuvia™ IMAC Cartridges | Pkg of 1, 5 ml | 7800811 |
Bio-Scale™ Mini Nuvia™ IMAC Cartridges | Pkg of 5, 5 ml | 7800812 |
Luer to BioLogic™ and NGC™ Systems Fittings Kit | 7320113 | |
Luer to M6 Adaptor Fittings Kit | 7320111 |
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