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Atomicforcemicroscopy(AFM)enableshigh-resolutionthree-dimensional(3D)imagingofcultured bone marrow-derivedmastcells.Cellswereimmobilizedbyaquickcentrifugationandfixationto preserve theirtransientcellularmorphologiesfollowedbyAFMcharacterizationinbuffer.This‘‘fix-and- look’’ approachpreservesthestructuralintegrityofindividualcells.Well-knownmembrane morphologies,suchasridgesandmicrovilli,arevisualized,consistentwithpriorelectronmicroscopy observations. Additionalinformationincludingthe3Dmeasurementsofthesecharacteristicfeatures are attainedfromAFMtopographs.Filopodiaandlamellopodia,associatedwithcellspreading,were capturedandvisualizedinthreedimensions.Newmorphologiesarealsorevealed,suchashigh-density ridges andmicro-craters.Thisinvestigationdemonstratesthatthe‘‘fix-and-look’’approachfollowedby AFM imagingprovidesaneffectivemeanstocharacterizethemembranestructureofhydratedcells with highresolution.Thequantitativeimagingandmeasurementspavethewayforsystematic correlation ofmembranestructuralfeatureswiththebiologicalstatusofindividualcells
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