人结核杆菌IgG（TB-IgG）试剂盒使用说明书

Human TB-IgG FOR RESEARCH USE ONLY Drug Names Generic Name：Human TB-IgG ELISA Kit. Purpose This kit allows for the determination of TB-IgG concentrations in Human serum, and other biological fluids. Principle of the assay The kit assay TB-IgG level in the sample，use Purified antigen to coat microtiter plate wells, make solid-phase antibody, then add TB-IgG to wells, Combined With TB-IgG, after washing and removing non-combinative antibody and other components ,then Combined antigen which with HRP labeled become antigen – antibody - enzyme- antigen complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge TB-IgG exist in the sample or not.