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Rat Activated protein C(APC)ELISA Kit FOR RESEARCH USE ONLY INTRODUCTION Assay range:0.1 ng/ml - 4 ng/ml 96 determinations Purpose This kit allows for the determination of APC concentrations in Rat serum, cell culture supernates and other biological fluids PRINCIPLE OF TEST The kit assay Rat APC level in the sample, use Purified Rat APC antibody to coat microtiter plate wells, make solid-phase antibody, then add APC to wells, Combined APC antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat APC in the samples is then
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