Rat Angiopoietin-1 (Ang-1)

Rat Angiopoietin-1 (Ang-1) FOR RESEARCH USE ONLY Assay range：50pg/ml- 2600pg/ml 96 determinations Purpose This kit allows for the determination of Ang-1 concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat Ang-1 level in the sample, use Purified Rat Ang-1 antibody to coat microtiter plate wells, make solid-phase antibody, then add Ang-1 to wells, Combined Ang-1 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat Ang-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Materials provided with the ki