锤子
Rat F1+2 FOR RESEARCH USE ONLY Assay range:6nmol/L -220 nmol/L 96 determinations Purpose This kit allows for the determination of F1+2 concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat F1+2 level in the sample, use Purified Rat F1+2 antibody to coat microtiter plate wells, make solid-phase antibody, then add F1+2 to wells, Combined F1+2 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm
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