锤子
Rat IL-1 FOR RESEARCH USE ONLY CATALOG #:R7468 Assay range:8ng/L-180ng/L 96 determinations Purpose This kit allows for the determination of IL-1 concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat IL-1 level in the sample , use Purified Rat IL-1 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-1 to wells, Combined IL-1 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution
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