Goat interleukin 1(IL-1)

Goat interleukin 1(IL-1) FOR RESEARCH USE ONLY Assay range：1.2ng/L -50 ng/L 96 determinations Purpose This kit allows for the determination of IL-1 concentrations in Goat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Goat IL-1 level in the sample，use Purified Goat IL-1 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-1 to wells, Combined IL-1 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Goat IL-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Materials provided with the kit 1 wash solution 20ml×1bottle