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Goat interleukin 8(IL-8) FOR RESEARCH USE ONLY Assay range:1.6ng/L - 70ng/L 96 determinations Purpose This kit allows for the determination of IL-8 concentrations in Goat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Goat IL-8 level in the sample, use Purified Goat IL-8 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-8 to wells, Combined IL-8 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Goat IL-8 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Materials provided with the kit
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