小鼠肿瘤坏死因子α(TNF-α)ELISA试剂盒

Mouse Tumor necrosis factor α(TNF-α) FOR RESEARCH USE ONLY Assay range：25 ng/L-800 ng/L 96 determinations Purpose This kit allows for the determination of TNF-α concentrations in Mouse serum. Principle of the assay The kit assay Mouse TNF-α level in the sample, use Purified Mouse TNF-α antibody to coat microtiter plate wells, make solid-phase antibody, then add TNF-α to wells, Combined TNF-α antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Mouse TNF-α in the samples is then determined by comparing the O.D. of the samples to the standard curve.