毛细管液相色在分析抗体及蛋白质等生物样品方面的应用

Liquid chromatographic (LC) separation of peptides and small proteins has been successfully performed for at least two decades, while the reversed-phase (RP) separation of large biomolecules, such as antibodies, fusion proteins, and enzymes has experienced more modest development. Peptides act chromatographically much like small drug molecules and that empirical base has an extensive literature for the analyst to employ. The advent of biotechnology and the need for analytical techniques to examine protein-based therapeutic agents has resulted in the RP of large proteins being studied more carefully.