方案摘要
方案下载应用领域 | |
检测样本 | |
检测项目 | |
参考标准 |
RNA helical junctions are common in RNA structures, including ribozymes, mRNA untranslated regions, rRNAs, and regulatory RNAs.?RNA junctions are also in complex with proteins.?However, little was known about the thermodynamic forces that drive the folding of RNA junctions, and it is difficult to dissect these forces for several reasons, including non-two-state folding, secondary and tertiary folding components are superimposable, and opposing enthalpic and entropic contributions to RNA folding.?The authors used Isothermal Titration Calorimetry (ITC) to study the folding of hammerhead ribozyme, a three-way RNA helical junction.?In their work, they had RNA in both the ITC cell and syringe, and during titration, both RNA stands annealed to form complete folded bimolecular ribozyme. This approach was different than other published ITC research on hammerhead ribozymes, which titrated concentrated MgCl2 into unimolecular hammerhead ribozyme, and measured Mg2+ binding (Hammann, C., Cooper, A., Lilley, D.M., Biochemistry 40, 1423-1429, 2001).
毛细流孔隙仪在均热板毛细液芯性能测试的应用
赛默飞世尔FlashSamrt 元素分析仪对于煤焦油样品中CHNS/O的分析
布鲁克3D XRM 正品飞天茅台挑选记
相关产品
麦奇克激光粒度仪 S3500系列
Rudolph AUTOPOL VI高精度旋光仪
Rudolph数字式密度计DDM 2911
全自动氨基酸分析仪 Biochrom 30+
纳米颗粒跟踪分析仪(生物纳米追踪)
流式影像仪 FlowCam®8400(cyano)
荷兰TE硫氮分析仪 XPLORER-V系列
自动液体处理系统
TURBISCAN 稳定性分析仪(多重光散射仪)
TURBISCAN 稳定性分析仪(多重光散射仪)
全自动比表面分析仪 (动态/静态法)
荷兰TE 总氯分析仪XPLORER TX
荷兰TE 总硫分析仪XPLORER TS
荷兰TE 总有机卤素分析仪XPLORER AOX
荷兰TE 总硫总氮分析仪XPLORER NS
关注
拨打电话
留言咨询