蔬菜null检测 | 非监督的MS/MS二级谱图的相似性代谢轮廓图分析显示与防御有关的土豆代谢物-布鲁克·道尔顿(Bruker Daltonics)

方案摘要

方案下载

应用领域	食品/农产品
检测样本	蔬菜
检测项目
参考标准	/

非监督的MS/MS二级谱图的相似性代谢轮廓图分析快速找出了17种以前未曾分析出的代谢物，并发现了羟基肉桂酰胺在AtACT-AtdTX18超表达路径中的合成机制。

方案下载

方案详情

Solanum tuberosum (potato) wildtype and overexpression lines with an enhanced Phytophtora infestans resistance were analysed for their metabolic response to simulated herbivory.

• Using the data acquired with Bruker LC-ESI-Q-TOF MS and MS/MS instrumentation and methods, the MetaboScape software provided an efficient platform for the straightforward linkage of structural similarities and metabolite abundances. We used various statistical tools (PCA, t-test) in MetaboScape to focus on relevant features. The unsupervised MS/MS similarity metabolite profiling approach enabled a rapid annotation of 17 metabolites which were previously unknown in our analyses.

• The common abundance patterns together with common MS/MS patterns pointed to an overflow of the hydroxycinnamic acid amide biosynthesis in the AtACT-AtDTX18 overexpression lines. This is in perfect accordance with the function of the overexpressed enzyme AtACT that is responsible for the biosynthesis of four HCAAs. The increase of the many other HCAAs might be a metabolic side effect of the plant to compensate for this overflow and to bypass toxic effects for the cell. MetaboScape was very valuable for easy data processing, the annotation of many formerly unknown compounds and an intuitive visualization of metabolic changes.

上一篇 4D-脂质组学高通量工作流程

下一篇组学研究加速生化制品生产效率

文献贡献者

布鲁克·道尔顿(Bruker Daltonics)

金牌会员丨第22年

查看全部资料